<i>In vitro</i> regeneration through callus in pointed gourd (<i>Trichosanthes dioica</i> Roxb.)

Abstract

An efficient protocol was developed for in vitro plant regeneration and multiplication through callus culture in pointed gourd. Among the explants, highest percentage of cotyledon explants (92.00%) produced callus when this explant cultured in MS medium supplemented with NAA (0.1, 0.5, 1.0, 1.5, and 2.0 mg/l) and 2, 4-D (0.1, 0.5, 1.0, 1.5, and 2.0 mg/l). The highest number of shoots per explant was observed in MS + 0.5 mg/l BAP + 0.5 mg/l NAA followed by 1.0 mg/l BAP + 0.5 mg/l NAA when inter-node derived callus cultured in MS medium. Among the explants derived calli from leaf, inter-node and cotyledon in in vitro regeneration study, inter-node appeared as the most suitable explant for callusing and plant regeneration. The best response towards root induction was achieved on half MS medium supplemented with 0.5 mg/l NAA. The regenerated plantlets were successfully established in prepared earthen soil pot.

Evaluation of phytochemicals and antibacterial activity of leaf and leaf derived callus extracts of Artemisia annua L. and Sauropus androgynus (L.) Merr.

“…Among the individual growth regulators used, maximum callus induction of 52% was observed in MS medium supplemented with NAA at 1 mg/ l concentration in S. androgynus, whereas 51% callus induction was observed in A. annua supplemented with 2,4-D at 1.5 mg/ l (Table 1). Many researchers have reported the use of 2,4-D, NAA and BAP for callus induction in various other medicinal plants (Malek et al, 2010;Usman et al, 2011;Thiruvengadam et al, 2012;AL-Hussaini et al, 2015;Bakhtiar et al, 2016). Further, it was also observed that, when the plant materials were grown on MS medium supplemented with combinations of growth regulators the percent callus induction was higher when compared to individual growth hormones supplementation.…”