<i>In vitro</i> micropropagation of banana

Abstract

The present study was conducted at the Biotechnology Laboratory, Biotechnology Division, Bangladesh Agricultural Research Institute (BARI), Gazipur during the period from September 2004 to June 2005 to investigate the effect of different concentrations of BAP and NAA on virus free plant regeneration, shoot multiplication and different concentrations of IBA and IAA on in vitro root formation of banana cv. BARI Banana-I. The culture meristem first turned brown in colour in 4-5 days which grew into a green globular hard coat mass after 30-35 days. From this ball like structure, adventitious plantlets were developed. Among the different concentrations, 7.5 mg/l BAP + 0.5 mg/l NAA showed highest shoot proliferation of 0.75, 2.75 and 6.25 shoots per explant at 10, 20 and 30 DAI, respectively. The longest shoot (1.03, 2.45 and 3.38 cm) at 10, 20 and 30 DAI, respectively, was produced by the treatment combination of 7.5 mg/l BAP + 0.5 mg/l NAA. The maximum number of leaves (2.50, 3.25 and 7.00 leaves/explant at 10, 20 and 30 DAI) were produced on the medium supplemented with the same treatment and it also produced the longest leaves, 0.85, 2.70 and 4.23 cm at 10, 20 and 30 DAI, respectively. For root initiation half strength MS medium supplemented with different levels of IBA (0, 0.5, 1 .0 and 1.50 mg/l) and IAA (0, 0.5 and 1.0 mg/l) was used. Root numbers varied with different concentrations of IBA and IAA. The highest number of roots were produced by 0.5 mg/l IAA + 0.5 mg/l IBA. The highest length (2.93, 4.63 and 5.88 cm) was recorded at 10, 20 and 30 DAI in the same treatment which was statistically significant. Meristem derived plantlets were transferred to poly bags containing 1:1 (ground soil : cowdung) mixture after 7 days hardening in room temperature (28-30°C) and established plantlet was ready for planting. Key Words: Banana; regeneration; micropropagation; plantlet. DOI: 10.3329/bjar.v34i4.5840Bangladesh J. Agril. Res. 34(4) : 645-659, December 2009

The Effects of Auxins and Cytokinin on Growth and Development of (&lt;i&gt;Musa&lt;/i&gt; sp.) Var. “Yangambi” Explants in Tissue Culture

“…These findings did not agree with the results of Al-Amin et al [25], where the MS media supplemented with BAP and NAA in their study showed different results for increasing shoot length Interactive effect of BAP and IAA was also observed in fresh weight (Figure 2). The media with 6 mg/l of BAP in combination with 0.35 mg/l IAA, significantly (p ≤ 0.01) resulted in high fresh weight as compared with other treatments ( Table 3).…”

Effect of Medium and Cytokinin Types on Banana Micropropagation during Multiplication Stage

“…The above mentioned results are in agreement with those of Gübbük and Pekmezci (2004), Gubbuk and Pekmezci (2006), Kalimuthu et al (2007), Shiragi et al (2008), Al-Amin et al (2009), Bhosale et al (2011), Mahadev et al (2011, Jafari et al (2013), Rahman et al (2013), Anbazhagan et al (2014), Mahdi et al (2014), Ngomuo and Ndakidemi (2014) and Shankar et al (2014). All of them found that BAP and kinetin increased the shoot number initiated from shoot tips of banana during micropropagation through tissue culture.…”

An efficient protocol for large-scale plantlet production from male floral meristems of Musa spp. cultivars Virupakshi and Sirumalai

“…Long generation time, various levels of ploidy, lack of genetic variability, and sterility of most edible cultivars have hampered the development of disease-resistant Musa by conventional breeding (Stover and Buddenhagen 1986;Stover and Simmonds 1987;Swennen and Vuylsteke 1993;Musoke et al 1999;Pillay et al 2004;Tripathi et al 2004). In vitro micropropagation has played a key role in clonal propagation of banana for obtaining large numbers of homogenous plants and breeding of plantains and bananas (Pierik 1987;Rowe and Rosales 1996;Vuylsteke et al 1997;Kalimuthu et al 2007;Al-Amin et al 2009). …”