&lt;i&gt;IN VITRO&lt;/i&gt; INHIBITION OF CELERY (&lt;i&gt;Apium graveolens&lt;/i&gt; L.) EXTRACT ON THE ACTIVITY OF XANTHINE OXIDASE AND DETERMINATION OF ITS ACTIVE COMPOUND

Iswantini, Dyah; Ramdhani, Tuti Hayati; Darusman, Latifah Kosim

doi:10.22146/ijc.21338

Cited by 17 publications

(10 citation statements)

References 14 publications

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“…Total phenolic and flavonoids contents are found to positively correlated with total antioxidant capacity of apiaceous plants. 17 However, in the current study we found by EPR spectroscopy that ADSh and MPR were to be a venerable hydroxyl radical scavenger even in low concentration, although it's TPC and TFC weren't the highest. Also, the low hydroxyl radical quenching capacity at low concentration attributed to ADF was expected as it possesses the low total phenol content but moderate flavonoid content was observed.…”

Section: Discussioncontrasting

confidence: 66%

“…Iswantini et al inferred that extract proved to be a potential scavenger for free OH• radical when it has the percent inhibition value greater than 50%. 17 At 10 mg/ml, all the tested extracts were quenched EPR signals from DMPO-HO• adduct (more than 85%), but at range 0.5-2 mg/ml signals scavenged to varying degrees. Methanol parsley roots extract (MPR) was perplexing in prohibiting formation of the DMPO-HO• adduct by 98.90 % at 10 mg/ml concentration.…”

Section: In Vitro Hydroxyl Radical (•Oh) Scavenging Ability Using Eprmentioning

confidence: 89%

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Comparative Antioxidant Activities of Selected Apiaceous Plants Using EPR Technique

Emad¹,

Ali²,

Meselhy³

et al. 2019

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MATERIALS AND METHODS Chemicals and reagents Folin-Ciocalteu reagent, gallic acid, and rutin standards were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). Aluminum chloride hexahydrate, methanol, and sodium carbonate were obtained from Fisher Scientific (Fair Lawn, NJ, USA). Water was purified using a Milli-Q system (Millipore). Hypoxanthine (HX) reagent was prepared by dissolving 13.6 mg of HX (Sigma H-9377) in 9.7 ml of distilled water and 0.3 ml of 1.0 M NaOH to obtain a concentration of 10mM to be stored at-20°C. Xanthine Oxidase (XO) reagent was prepared by mixing 490 µl of XO with 10 µl PBS ABSTRACT Introduction: Electron Paramagnetic Resonance (EPR) spectroscopy is a unique technique able to identify and quantify free radicals in the complex biological matrices. In this study, free radical scavenging activity of aqueous and methanol extracts of fruits, shoots and roots of dill and parsley was examined using EPR technique, in addition to the determination of their contents of total polyphenols (TPC) and flavonoids (TFC). Method: The hydroxyl anion scavenging activity (HASA) of the tested extracts was determined using EPR spin trapping technique and hypoxanthine/xanthine oxidase system generating (O•-2). The TPC and TFC were determined using Folin-Ciocalteu and aluminum chloride colorimetric assays. Results: Both aqueous shoot of dill (ADSh) and methanol root of parsley (MPR) demonstrated the strongest inhibition of HASA. On the contrary, the aqueous extract of dill fruit at a concentration of 0.5 mg/ml was found to be pro-oxidant (49.43 %), but at 10 mg/ml demonstrated potent inhibition of HASA (98.12 %). TPC was found to be the highest in the methanol extract of parsley fruit (MPF, 88.62 ± 0.6 mg GAE/g) and the lowest in MPR (4.34 ± 0.050 mg GAE/g). On the other hand, TFC was the highest in MPF (584.29 ± 2.10 mg GAE/g) and the lowest in the methanol extract of dill fruit (MDF, 1.28 ± 0.02 mg rutin/g). Conclusion: In this study, EPR provided a direct insight that all tested apiaceous plants extracts showed effective HASA except two extracts that proved to be pro-oxidant at 0.5 mg/mL This study confirmed that there is no correlation between antioxidant potential and TPC and TFC.

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Section: Discussioncontrasting

confidence: 66%

Section: In Vitro Hydroxyl Radical (•Oh) Scavenging Ability Using Eprmentioning

confidence: 89%

Comparative Antioxidant Activities of Selected Apiaceous Plants Using EPR Technique

Emad¹,

Ali²,

Meselhy³

et al. 2019

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“…Based on (Table I) shows that the addition of celery stems infused water extract has lower uric acid uptake compared to the leaves infused water extract. Iswantini (2012) tested the roots of celery and their plants using maceration methods. The results of the Iswantini (2012) study show that the lower part of the plant, the celery root has a better inhibition activity than the plant part.…”

Section: Resultsmentioning

confidence: 99%

Antihyperurisemic Activity of Aqueous Celery Infusion by Xanthine Oxidase Enzyme Inhibition

Zummah

Martha

2018

Trad.Med.J

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Hyperuricemia is a metabolic disorder because of excessive uric acid production. The high prevalence of hyperuresemia in the world, especially in Indonesia makes the research of antihyperurisemia drugs very needed today. The purpose of this study was to determine the activity of water infusion extract from celery stems and leaves to inhibited xanthine oxidase enzyme. Xanthine oxidase enzyme plays a role in change of hypoxanthine to xanthine and then becomes uric acid. The activity of xanthine oxidase enzyme was determined by UV spectrophotometry method and using xanthine substrate. Absorption at 290 nm indicates the presence of a uric acid product from xanthine oxidation by the xanthine oxidase enzyme. Infused extract which has the smallest absorption at 290 nm showed a better inhibitory ability of the xanthine oxidase enzyme because the change of xanthine substrate to uric acid is inhibited. The result showed the celery stems water infusion extract had better inhibition activity than celery leaves water infusion extract. The best inhibition percentage of celery stems water infusion was 90,25%, while the celery leaves water infusion was 69.07% both of at 20% concentration. The type inhibition of celery stems water infusion extract to xanthine oxidase enzyme showed an uncompetitive inhibition type because showed change in Km and Vmax values. Km and Vmax values before the addition of water infusion of celery stems were 104.33 ppm and 3.83 U/mL respectively changed to 74.49 ppm and 2.69 U/mL after the addition of extract.

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“…Table 2 shows the concentration‐dependent hydroxyl radical scavenging potency of A. graveolens extracts expressed as % inhibition of the EPR‐detected DMPO‐HO• radical adduct. Crude extracts and/or fractions exhibited potential scavenging effect for free •OH radical when exhibiting a percent inhibition value greater than 50% (Iswantini, Ramdhani, & Darusman, 2012). At 10 mg/ml, all Apium extracts were able to quench EPR signals due to DMPO‐HO• adduct, but at lower doses of 0.5–2 mg/ml signals scavenged to varying degrees.…”

Section: Resultsmentioning

confidence: 99%

Anti‐inflammatory and antioxidant effects of Apium graveolens L. extracts mitigate against fatal acetaminophen‐induced acute liver toxicity

et al. 2020

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In the present work, antioxidant activity, total phenolics (TP), and total flavonoids (TF) contents of aqueous and methanol extracts of celery were determined, in addition to untargeted metabolites profiling its methanol celery root extract (MCRE) via UPLC-MS. Although MCRE exhibited the lowest TPC and TFC levels, it presented the most potential hydroxyl radical quenching effect using electron paramagnetic resonance spin trapping technique. Treatment of Acetaminophen-induced hepatotoxicity (AAH) rats with MCRE lowered serum levels of AST, ALT, ALP, TNF-α, and IL-1β significantly. Additionally, MCRE significantly increased total antioxidant capacity (TAC) and glutathione (GSH) levels relative to AAH rats. Strikingly, Kaplan-Meier survival analysis of all groups revealed a 100% prevention of acetaminophen-induced mortality of rats by MCRE pretreatment (100 mg/kg/day). MCRE prevented AAHassociated severe weight loss and elicited normal behavior in the rescued rats. Our results suggest that pretreatment with MCRE can mitigate against overdosed acetaminophen-induced acute liver failure and warrant further investigations on the potential of postinjury intervention.

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<i>IN VITRO</i> INHIBITION OF CELERY (<i>Apium graveolens</i> L.) EXTRACT ON THE ACTIVITY OF XANTHINE OXIDASE AND DETERMINATION OF ITS ACTIVE COMPOUND

Abstract: ABSTRACT

Cited by 17 publications

References 14 publications

Comparative Antioxidant Activities of Selected Apiaceous Plants Using EPR Technique

Comparative Antioxidant Activities of Selected Apiaceous Plants Using EPR Technique

Antihyperurisemic Activity of Aqueous Celery Infusion by Xanthine Oxidase Enzyme Inhibition

Anti‐inflammatory and antioxidant effects of Apium graveolens L. extracts mitigate against fatal acetaminophen‐induced acute liver toxicity

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