&lt;I&gt;In Vitro&lt;/I&gt; Cytotoxicity of Poly(Methyl Methacrylate) Nanoparticles and Nanocapsules Obtained by Miniemulsion Polymerization for Drug Delivery Application

Feuser, Paulo Emílio; Lorena, Dos Santos Bubniak; Nascimento, Bodack Camila do; Alexsandra, Valerio; Santos-Silva, Maria Cláudia; Eduardo, Ricci‐Junior; Sayer, Cláudia; Hermes, DE Araujo Pedro Henrique

doi:10.1166/jnn.2016.11610

Cited by 21 publications

(12 citation statements)

References 15 publications

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“…Non-magnetic core-shell nanoparticles (PMMA/PVPi) presented an average cell viability of 84%; however, the highest cell viability very close control was approximately 99% observed for concentration of 0.10 mg•mL −1 . Feuser et al [61] evaluated in vitro cytotoxicity in murine fibroblast (L929) and normal human fibroblast (NIH-3T3) by MTT assay for 24 h of PMMA nanoparticles and nanocapsules obtained by miniemulsion polymerization. Both cells presented high cell viability, between 81 to 84% even at highest tested concentration of 750 μg•mL −1 .…”

Section: Resultsmentioning

confidence: 99%

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Well Defined Poly(Methyl Methacrylate)-Fe3O4/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells

et al. 2020

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The objective of this work is to develop and characterize polymeric nanoparticles with core–shell morphology through miniemulsion polymerization combined with seeded emulsion polymerization, aiming at the application in the treatment of vascular tumors via intravascular embolization. The synthesis of the core–shell nanocomposites was divided into two main steps: (i) Formation of the core structure, consisting of poly(methyl methacrylate)/magnetic oxide coated with oleic acid (OM-OA) via miniemulsion and (ii) shell structure produced through seeded emulsion polymerization of vinyl pivalate. Nanocomposites containing about 8 wt.% of OM-OA showed high colloidal stability, mean diameter of 216.8 nm, spherical morphology, saturation magnetization (Ms) of 4.65 emu·g−1 (57.41 emu·g−1 of Fe3O4), preserved superparamagnetic behavior and glass transition temperature (Tg) of 111.8 °C. TEM micrographs confirmed the obtaining of uniformly dispersed magnetic nanoparticles in the PMMA and that the core–shell structure was obtained by seeded emulsion with Ms of 1.35 emu·g−1 (56.25 emu·g−1 of Fe3O4) and Tg of 114.7 °C. In vitro cytotoxicity assays against murine tumor of melanoma (B16F10) and human Keratinocytes (HaCaT) cell lines were carried out showing that the core–shell magnetic polymeric materials (a core, consisting of poly(methyl methacrylate)/Fe3O4 and, a shell, formed by poly(vinyl pivalate)) presented high cell viabilities for both murine melanoma tumor cell lines, B16F10, and human keratinocyte cells, HaCaT.

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Section: Resultsmentioning

confidence: 99%

“…Feuser et al [61] evaluated in vitro cytotoxicity in murine fibroblast (L929) and normal human fibroblast (NIH-3T3) by MTT assay for 24 h of PMMA nanoparticles and nanocapsules obtained by miniemulsion polymerization. Both cells presented high cell viability, between 81 to 84% even at highest tested concentration of 750 µg•mL −1 .…”

Section: Resultsmentioning

confidence: 99%

Well Defined Poly(Methyl Methacrylate)-Fe3O4/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells

et al. 2020

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“…This lower surface charge can be related to the presence of OA on the MNPs' surface, suggesting a higher concentration of surfactant adsorbed on the PTEe-MNPs nanoparticles surface. 44,45 TEM images allowed to verify the presence of dark dots (MNPs) encapsulated in the PTEe nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the in vivo acute toxicity of poly(thioether‐ester) and superparamagnetic poly(thioether‐ester) nanoparticles obtained by thiol‐ene miniemulsion polymerization

et al. 2021

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Poly(thioether-ester) (PTEe) nanoparticles obtained by thiol-ene polymerization have received attention of many researchers due to several advantages, including, biocompatibility and biodegradability. The search for new nanomaterials requires toxicity studies to assess potential toxic effects of their administration. Therefore, the aim of this study was to evaluate the in vivo acute toxicity of PTEe and poly(thioetherester)-coated magnetic nanoparticles prepared by thiol-ene polymerization in miniemulsion. These nanoparticles presented a mean size of approximately 120 nm, spherical morphology, and negative surface charge. Doses of 40 mg/kg were administered intraperitoneally to Swiss mice and nociceptive, behavioral and biochemical parameters were investigated in five different organs. None of the nanoparticles led to any alterations in the nociceptive and behavioral responses. Biochemical alterations were observed in liver, decreasing the sulfhydryl and glutathione (GSH) levels, suggesting the dependence of the GSH metabolism in the elimination of the nanoparticles. In general, both nanoparticle types did not cause disturbances in biochemical parameters analyzed in others organs. These results suggest that both nanoparticle types did not induce acute toxicity to the different organs evaluated, reinforcing the biocompatibility of PTEe nanoparticles synthetized by thiol-ene polymerization.

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“…The evaluation of the morphology and size of mats is fundamentally important when it is desired to obtain polymeric systems for biomedical applications on cellular and biological processes. [36] Figure 2 displays the SEM images of the samples electrospun with chloroform (PCLÀ T) and ionic liquid (PCL-IL). The mats structure consists of a set of non-woven seamless fibers, of circular shape in cross-section, with fully interconnected porosity.…”

Section: Characterization and Morphologymentioning

confidence: 99%

Electrospun Polycaprolactone Scaffolds Using an Ionic Liquid as Alternative Solvent: Morphometric, Mechanical and Biological Properties

et al. 2020

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Ionic liquids (IL) are a greener alternative to aggressive organic solvents used for biopolymers dissolution in the electrospinning process. Nevertheless, the effect of IL on the properties of the electrospun materials is still a gap. In this work, we obtain polycaprolactone (PCL) electrospun mats using two solvents, either trichloromethane (CHCl 3)-a conventional toxic and carcinogenic solvent-or 1-butyl-3-methylimidazolium chloride ([Bmim]Cl), an ionic liquid. The differences between the respective electrospun products were assessed through the scaffold's morphology, mechanical properties and cytotoxicity. The use of IL resulted in the formation of textured thinner fibers, enhancing the surface area in 43 %. IL induced a lower rupture stress and elongation of the mats of 34 and 39 %, respectively, while the elastic modulus and yield stress did not present significant differences. The cytotoxicity assays using L929 cells detected a number of viable cells slightly higher for the scaffold produced with IL. Cell interaction tests also revealed that L929 cells adhere and proliferate on both scaffolds. However, the cell adhesion was higher for the mats fabricated using IL. Beyond the results, this work sheds light on the pros and cons of tissue engineering scaffolds produced with the aid of less hazardous solvents.

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<I>In Vitro</I> Cytotoxicity of Poly(Methyl Methacrylate) Nanoparticles and Nanocapsules Obtained by Miniemulsion Polymerization for Drug Delivery Application

Cited by 21 publications

References 15 publications

Well Defined Poly(Methyl Methacrylate)-Fe3O4/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells

Well Defined Poly(Methyl Methacrylate)-Fe3O4/Poly(Vinyl Pivalate) Core–Shell Superparamagnetic Nanoparticles: Design and Evaluation of In Vitro Cytotoxicity Activity Against Cancer Cells

Evaluation of the in vivo acute toxicity of poly(thioether‐ester) and superparamagnetic poly(thioether‐ester) nanoparticles obtained by thiol‐ene miniemulsion polymerization

Electrospun Polycaprolactone Scaffolds Using an Ionic Liquid as Alternative Solvent: Morphometric, Mechanical and Biological Properties

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