&lt;i&gt; In Vitro&lt;/i&gt; Control of Tomato (&lt;i&gt;Solanum lycopersicon&lt;/i&gt; L.) Fruit Rot Caused by Fungi Using Two Plant Extracts

Okey, Edward Ntui; Akwaji, Patrick Ishoro; Akpan, Juliet Bassey; Umana, Etim Johnson; Bassey, Glory Akpan

doi:10.18052/www.scipress.com/ilns.52.19

Cited by 1 publication

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References 15 publications

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“…To date, most researchers have focused on V. amygdalina crude extract in order to uncover its potential as an antifungal agent for the management of plant disease. Recent findings found that V. amygdalina ethanol extracts showed a good ability to inhibit postharvest fungal pathogens Rhizopus stolonifer and Fusarium moniliforme [ 16 , 17 ]. In another study, in an in vitro test using an ethanol crude extract of V. amygdalina at 300 mg/mL, the growth of Cercosporella persica and Curvularia lunatus were completely inhibited [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Antifungal Activity and Phytochemical Screening of Vernonia amygdalina Extract against Botrytis cinerea Causing Gray Mold Disease on Tomato Fruits

Yusoff

Haron

Mohamed

et al. 2020

Biology

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Gray mold disease caused by Botrytis cinerea is a damaging postharvest disease in tomato plants, and it is known to be a limiting factor in tomato production. This study aimed to evaluate antifungal activities of Vernonia amygdalina leaf extracts against B. cinerea and to screen the phytochemical compound in the crude extract that had the highest antifungal activity. In this study, crude extracts of hexane, dichloromethane, methanol, and water extracts with concentration levels at 100, 200, 300, 400, and 500 mg/mL were shown to significantly affect the inhibition of B. cinerea. Among the crude extracts, dichloromethane extract was shown to be the most potent in terms of antifungal activities. The SEM observation proved that the treatment altered the fungal morphology, which leads to fungal growth inhibition. For the in vivo bioassay, the fruits treated with dichloromethane extract at 400 and 500 mg/mL showed the lowest disease incidence with mild severity of infection. There were 23 chemical compounds identified in V. amygdalina dichloromethane extract using GCMS analysis. The top five major compounds were dominated by squalene (16.92%), phytol (15.05%), triacontane (11.31%), heptacosane (7.14%), and neophytadiene (6.28%). Some of these significant compounds possess high antifungal activities. This study proved that V. amygdalina from dichloromethane extract could be useful for inhibiting gray mold disease on tomato fruit and has potential as a natural antifungal agent.

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Section: Introductionmentioning

confidence: 99%