&lt;i&gt;In vitro&lt;/i&gt; antisickling and antioxidant properties of aqueous and ethanol extracts of fifty selected plants used in the management of sickle cell disorder in southern Nigeria

Amujoyegbe, O.O.; Idu, MacDonald; Agbedahunsi, Joseph M.; Obuotor, Efere Martins; Bazuaye, GN

doi:10.4314/njnpm.v20i1.23

Nigerian Journal of Natural Products and Medicine

2017

DOI: 10.4314/njnpm.v20i1.23

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<i>In vitro</i> antisickling and antioxidant properties of aqueous and ethanol extracts of fifty selected plants used in the management of sickle cell disorder in southern Nigeria

O.O. Amujoyegbe¹,

MacDonald Idu²,

Joseph M. Agbedahunsi³

et al.

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2021

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Isolation and characterisation of quercitrin as a potent anti-sickle cell anaemia agent from Alchornea cordifolia

Adeniyi¹,

Baptista²,

Bhowmick³

et al. 2021

Preprint

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Alchornea cordifolia Müll. Arg. (commonly known as Christmas Bush) has been used traditionally in Africa to treat sickle cell anaemia (a recessive disease, arising from the S haemoglobin [Hb] allele) but the active compounds are yet to be characterised. Herein we describe the use of sequential fractionation coupled with in vitro anti-sickling assays to purify the active component. Sickling was induced in HbSS genotype blood samples using sodium metabisulphite (Na2S2O5) or incubation in 100 % N2. Methanol extracts of A. cordifolia leaves and its sub-fractions showed >70 % suppression of HbSS erythrocyte sickling. Purified compound demonstrated 87.2 ± 2.39 % significant anti-sickling activity and 93.1 ± 2.69 % erythrocyte sickling-inhibition at 0.4 mg/mL. Nuclear magnetic resonance (NMR) spectra and high-resolution mass spectroscopy identified it as quercitrin (quercetin 3-rhamnoside). Purified quercitrin also inhibited the polymerisation of isolated HbS and stabilized sickle erythrocytes membranes. Metabolomic comparisons of blood samples using flow-infusion electrospray-high resolution mass spectrometry indicated that quercitrin could convert HbSS erythrocyte metabolomes to be similar to HbAA. Sickling was associated with changes in anti-oxidants, anaerobic bioenergy and arachidonic acid metabolism, all of which were reversed by quercitrin. The findings described could inform efforts directed to the development of an anti-sickling drug or quality control assessments of A. cordifolia preparations.

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Isolation and characterisation of quercitrin as a potent anti-sickle cell anaemia agent from Alchornea cordifolia

Adeniyi¹,

Baptista²,

Bhowmick³

et al. 2021

Preprint

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show abstract

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<i>In vitro</i> antisickling and antioxidant properties of aqueous and ethanol extracts of fifty selected plants used in the management of sickle cell disorder in southern Nigeria

Cited by 1 publication

References 0 publications

Isolation and characterisation of quercitrin as a potent anti-sickle cell anaemia agent from Alchornea cordifolia

Isolation and characterisation of quercitrin as a potent anti-sickle cell anaemia agent from Alchornea cordifolia

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