<i>In-vitro</i> Antimicrobial Activity of Roots of <i>Rauwolfia serpentina</i> L. Benth Kurz

Abstract

Microbial pathogens develop resistance to antibiotics after repeated administration during the treatment of infectious diseases. Therefore, it is necessary to find alternative antimicrobial drugs and the present trend is focused on medicinal plants. The hereby research work was carried out to investigate the antimicrobial activity of solvents as well as aqueous extracts of Rauwolfia serpentina roots. The extracts were tested against Bacillus subtilis, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae, Micrococcus luteus, Pseudomonas aeruginosa, Proteus vulgaris, Staphylococcus aureus, Streptococcus pneumoniae, Aspergillus niger, Candida albicans and Saccharomyces cerevisiae by agar well diffusion method. It was observed that methanol extract showed the highest antimicrobial activity against multi drug resistance S. aureus at 100 mg/ml concentration, while S. aureus was the most susceptible bacterium to all extracts. However, E. faecalis, M. luteus and S. pneumoniae were also susceptible to the experimented solvents and extracts. On the other hand, K. pneumoniae was resistant against the solvent and aqueous extracts. The present study suggested that methanol extracts of R. serpentina roots would be helpful in treating diseases caused by human pathogenic bacteria and fungi. In particular, based on the results obtained in the current experiment, it can be recommended for the control of infectious Gram-positive bacteria.

Antibacterial Activity of Bryophyllum pinnatum and Rauvolfia vomitoria on Neonatal Group B Streptococcus

“…These two solvents showed the most effective inhibition against GBS, with a mean inhibition diameter ranging from 15 mm to 23 mm. This finding aligns with the studies conducted by Eteng et al 34 and Mokutima et al 35 , but it contradicts the report by Owk & Lagudu 36 , who found that the methanol extract exhibited the highest activity against GBS. 32 , who also observed similar effects of these plants on GBS.…”

Comparative assessment of chemical compositions, antioxidant and antimicrobial activity in ten berries grown in China

“…The microorganisms were cultured in Nutrient Broth (beef extract 3 g, peptone 10 g, NaCl 5 g, agar 20 g, water 1 L, pH7.4‐7.6) for 16 h, under shaking . Disc diffusion method was used for screening the antimicrobial activity of all berries in order to determine the growth inhibition zones of microorganisms around certain berries . The Muller Hinton Agar (MAA) was dissolved in distilled water to make a concentration of 3.1 g/100 mL.…”

Differential Chromosome Banding of Three <i>Rauvolfia</i> Species

“…The increasing demand of various Rauvolfia species for medicinal uses has caused a serious reduction of the individual number in native populations due to over-exploitation, habitat loss and anthropogenic activities. As a result, several Rauvolfia species are now restricted in its natural habitat and have also been recognized as vulnerable (Owk and Lagudu 2016).…”