<i>In vitro </i>alteration of satellite association and nucleolar persistence in mitotic human lymphocytes

Abstract

Satellite association (SA) in lymphocytes obtained from women was found in 89.9% of cells after 48 h in culture and in 66.1% of cells after 96 h. After 48 h, 33.7% of acrocentrics participate in SA, whereas 30.7%, and 17.7% participate after 72 and 96 h. Prophase through prometaphase nucleoli were found in 26.8% of cells after 48 h and in 13.1% of cells after 96 h. SA and mitotic nucleoli are most common in 48 h cultures, and both decrease with increasing in vitro duration. These findings suggest a relationship between nucleolar persistence and SA.

High degree of satellite association and decreased nucleolar organizer activity in cystic fibrosis subjects

“…The interaction between age and colchicine concentration may explain some of the discrepancies found in the literature concerning the effect of several internal factors, such as age and sex [Zigmund et al, 1979;Hansson, 1979;Jacobs and Mayer, 198 I], or external factors, such as technical conditions [Back and Zang, 1969;Hansson, 1970a;Nankin, 1970;Ravia et al, 19851, on the frequency of SA. Since the degree of SA is usually determined in colchicine-or colcemid-arrested cells, the concentration of the drug and the duration of its application should be considered; moreover, conclusions should be based on data obtained from a wide range of drug concentrations.…”

The effect of cell kinetics and harvest time on SCE and NOR associations in <i>Macaca fuscata </i>lymphocytes

“…This decrease in NOR association frequency could be related to decreases in NOR activity (Warburton et al, 1976;Vogel et al, 1978), cell kinetics (Nankin, 1970;Mattevi and Salzano, 1975), or a possible pro gressive disruption of the original chromosome posi tion over time (Sigmund et al, 1979).…”

Some Aspects on the Variability of the Satellitized Arm, in Human Acrocentric Chromosomes

“…Heneen and Nichols (1966) and Nankin (1970) determined that the groups of acrocentric chromosomes observed in the metaphase are the result of chromosomal association in the interphase for the organization of common nu cleoli.…”

“…The culture of lymphocytes were made according to the technique of Moorhead et al (1960) with slight modifications: 30 minutes colchicine at 0.0002% and hy potonic pretreatment with 3:1 distilled water and were arrested at the 72 hours. The preparatory technique was maintained in every case as nearly identical as possible, to avoid the variation observed in the number of satellites attributable to the technique (Henneen and Nichols 1966, Ford and Woollan 1967, Zang and Bang 1968and Nankin 1970. The metaphases were chosen in consideration of the elon gation of most of the chromosomes, those in which the centromere of either acro centric chromosome was not easy to discern being eliminated.…”