&lt;i&gt;In Situ&lt;/i&gt; Hybridization Method Reveals (Pro)renin Receptor Expressing Cells in the Pituitary Gland of Rats: Correlation with Anterior Pituitary Hormones

Takahashi, Kazuhiro; Yatabe, Megumi; Fujiwara, Ken; Hirose, Takuo; Totsune, Kazuhito; Yashiro, Takashi

doi:10.1267/ahc.12030

Cited by 7 publications

(7 citation statements)

References 23 publications

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“…(P)RR is expressed in various types of cells, including neurons, endocrine cells, lymphocytes, cardiomyocytes, and renal tubular cells (Hirose et al 2009;Takahashi et al 2010aTakahashi et al , 2010bTakahashi et al , 2013Narumi et al 2015). We have previously reported expression of (P)RR in a human erythroblastic cell line, YN-1 and its clonal variant cell line, YN-1-0-A (Kaneko et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Expression of (Pro)renin Receptor During Rapamycin-Induced Erythropoiesis in K562 Erythroleukemia Cells and Its Possible Dual Actions on Erythropoiesis

Kaneko

Ohba

Hirose

et al. 2017

Tohoku J. Exp. Med.

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(Pro)renin receptor ((P)RR), a specific receptor for renin and prorenin, is expressed in erythroblastic cells. (P)RR has multiple biological actions: prorenin activation, stimulation of the intracellular signaling including extracellular signal-regulated kinases, and functional complex formation with vacuolar H + -ATPase (v-ATPase). However, the functional implication of (P)RR in erythroblast cells has not been clarified. The aim of the present study was to clarify changes of (P)RR expression during erythropoiesis and a role of (P) RR in the heme synthesis. (P)RR expression was studied during rapamycin-induced erythropoiesis in a human erythroleukemia cell line, K562. Treatment with rapamycin (100 nM) for 48 hours significantly increased %number of hemoglobin-producing cells, γ -globin mRNA levels, erythroid specific 5-aminolevulinate synthase (ALAS2) mRNA levels, and heme content in K562 cells. Both (P)RR protein and mRNA levels increased about 1.4-fold during rapamycin-induced erythropoiesis. Suppression of (P) RR expression by (P)RR-specific small interference RNA increased ALAS2 mRNA levels about 1.6-fold in K562 cells, compared to control using scramble RNA, suggesting that (P)RR may down-regulate ALAS2 expression. By contrast, treatment with bafilomycin A1, an inhibitor of v-ATPase, decreased greatly % number of hemoglobin-producing cells and heme content in K562 cells, indicating that the v-ATPase function is essential for hemoglobinization and erythropoiesis. Treatment with bafilomycin A1 increased (P) RR protein and mRNA levels. In conclusion, we propose that (P)RR has dual actions on erythropoiesis: the promotion of erythropoiesis via v-ATPase function and the down-regulation of ALAS2 mRNA expression. Thus, (P)RR may contribute to the homeostatic control of erythropoiesis.

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Section: Introductionmentioning

confidence: 99%

Expression of (Pro)renin Receptor During Rapamycin-Induced Erythropoiesis in K562 Erythroleukemia Cells and Its Possible Dual Actions on Erythropoiesis

Kaneko

Ohba

Hirose

et al. 2017

Tohoku J. Exp. Med.

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“…In addition, the effect of bafilomycin A1 on the cell growth inhibition and apoptosis was remarkably enhanced by hirsutanol A in MCF-7 breast carcinoma cells (34). (P)RR was widely expressed in various types of cells and tissues including brain, kidney, adrenal and adrenal tumors, and certain cultured tumor cells (7,8,11,20,(29)(30)(31)33). However, there has been no report on expression of (P)RR in breast carcinoma tissues.…”

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confidence: 99%

Expression of (pro)renin receptor in breast cancers and its effect on cancercell proliferation

et al. 2014

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ABSTRACT(Pro)renin receptor ((P)RR) is a specific receptor for renin and prorenin. The aim of the present study is to clarify expression of (P)RR and pathophysiological roles of (P)RR in human breast carcinomas. (P)RR expression was studied in 69 clinical cases of breast carcinoma by immunohistochemistry. Effects of (P)RR on cell proliferation were examined in cultured human breast carcinoma cells using (P)RR specific small interference RNA. Immunohistochemistry showed that (P)RR immunoreactivity was detected in the breast carcinoma cells in 50 of 69 cases of breast carcinoma (72%). The analysis on association between (P)RR immunoreactivity and clinicopathological parameters showed that the number of (P)RR positive cases was significantly greater in Ki-67 (a cell proliferation marker) ≥ 10% group than in Ki-67 < 10% group (P = 0.02). (P)RR was expressed in 4 types of human breast carcinoma cell lines. (P)RR specific small interference RNA inhibited proliferation of both MCF-7 (ERα positive) and SK-BR-3 (ERα negative) cells. The present study has shown, for the first time, the expression of (P)RR in human breast carcinoma tissues and cultured breast carcinoma cell lines. These findings have raised the possibility that the blockade of the (P)RR signaling may be a novel therapeutic strategy against breast carcinomas.The renin-angiotensin system (RAS) plays important roles, not only in homeostatic regulation of blood pressure and water-electrolyte metabolism, but also in tumor biology including breast cancers. All the components of the RAS, including prorenin, renin, angiotensinogen (AGT), angiotensin I converting enzyme (ACE) and angiotensin receptors were localized in normal and cancerous breast tissues (28). Angiotensin II (Ang II) acts on the angiotensin II type 1 receptor (AT1R) to promote cell proliferation in breast cancer cells, via the protein kinase C/Ca 2+ / phosphoinositide 3-kinase pathways, and extracellular signal-related kinase (ERK) activation (32). Ang II promoted cell adhesion, invasion and metastasis of breast carcinoma by regulating the expression of integrins, vascular endothelial growth factor and matrix metalloproteinase-9 (22, 23). Clinicopathological study showed that AT1R expression in breast carcinoma tissues correlated with axillary lymph node metastasis (2). (Pro)renin receptor ((P)RR), a specific receptor for renin and prorenin, consists of 350 amino acids

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“…Interestingly, AVP and oxytocin are shown to co-localize with (P)RR in the supraoptic and paraventricular nuclei in human and mouse hypothalamus [ 74 , 75 ]. Furthermore, a recent study using in situ hybridization for (P)RR mRNA and immunohistochemistry double-staining for the pituitary hormones showed that (P)RR mRNA was expressed in most of the GH cells and ACTH cells in the anterior lobe of the pituitary gland, strongly suggesting a central crosstalk between AVP release and (P)RR, but further investigation on this issue is demanded [ 76 ].…”

Section: Discussionmentioning

confidence: 99%

AVP-Induced Increase in AQP2 and p-AQP2 Is Blunted in Heart Failure during Cardiac Remodeling and Is Associated with Decreased AT1R Abundance in Rat Kidney

2015

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AimThe objective was to examine the renal effects of long-term increased angiotensin II and vasopressin plasma levels in early-stage heart failure (HF). We investigated the regulations of the V2 vasopressin receptor, the type 1A angiotensin II receptor, the (pro)renin receptor, and the water channels AQP2, AQP1, AQP3, and AQP4 in the inner medulla of rat kidney.MethodsHF was induced by coronary artery ligation. Sixty-eight rats were allocated to six groups: Sham (N = 11), HF (N = 11), sodium restricted sham (N = 11), sodium restricted HF (N = 11), sodium restricted sham + DDAVP (N = 12), and sodium restricted HF + DDAVP (N = 12). 1-desamino-8-D-arginine vasopressin (0.5 ng h-1 for 7 days) or vehicle was administered. Pre- and post-treatment echocardiographic evaluation was performed. The rats were sacrificed at day 17 after surgery, before cardiac remodeling in rat is known to be completed.ResultsHF rats on standard sodium diet and sodium restriction displayed biochemical markers of HF. These rats developed hyponatremia, hypo-osmolality, and decreased fractional excretion of sodium. Increase of AQP2 and p(Ser256)-AQP2 abundance in all HF groups was blunted compared with control groups even when infused with DDAVP and despite increased vasopressin V2 receptor and Gsα abundance. This was associated with decreased protein abundance of the AT1A receptor in HF groups vs. controls.ConclusionEarly-stage HF is associated with blunted increase in AQP2 and p(Ser256)-AQP2 despite of hyponatremia, hypo-osmolality, and increased inner medullary vasopressin V2 receptor expression. Decreased type 1A angiotensin II receptor abundance likely plays a role in the transduction of these effects.

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<i>In Situ</i> Hybridization Method Reveals (Pro)renin Receptor Expressing Cells in the Pituitary Gland of Rats: Correlation with Anterior Pituitary Hormones

Cited by 7 publications

References 23 publications

Expression of (Pro)renin Receptor During Rapamycin-Induced Erythropoiesis in K562 Erythroleukemia Cells and Its Possible Dual Actions on Erythropoiesis

Expression of (Pro)renin Receptor During Rapamycin-Induced Erythropoiesis in K562 Erythroleukemia Cells and Its Possible Dual Actions on Erythropoiesis

Expression of (pro)renin receptor in breast cancers and its effect on cancercell proliferation

AVP-Induced Increase in AQP2 and p-AQP2 Is Blunted in Heart Failure during Cardiac Remodeling and Is Associated with Decreased AT1R Abundance in Rat Kidney

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