&lt;i&gt;Fusarium&lt;/i&gt; spp. and levels of fumonisins in maize produced by subsistence farmers in South Africa

Ncube, Edson; Flett, B. C.; Waalwijk, C.; Viljoen, Altus

doi:10.4102/sajs.v107i3/4.367

Cited by 1 publication

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References 14 publications

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“…A highly significant relationship was recorded between mean target DNA using qPCR and mean fumonisin concentrations with a R 2 =0.63, P=0.000. This corresponds with the findings of Ncube et al (2011) who reported a poor correlation between F. verticillioides quantified by the plating out method and target DNA of Fusarium spp. determined by qPCR (r=0.14, p≤0.05).…”

Section: Correlation Between Qpcr Hplc and The Plating Out Methodssupporting

confidence: 91%

“…and associated fumonisin levels in maize in South African production areas. Fumonisins in maize produced by subsistence farmers in northern KwaZuluNatal (Zululand) province of South Africa exceeded 2 ppm set by the United States Food and Drug Administration in 52 % and 17 % of samples collected in 2006 and 2007 respectively (Ncube et al 2011). These authors also reported a number of samples from Mokopane (Limpopo) and Lusikisiki (Eastern Cape) containing excessive fumonisin levels.…”

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confidence: 98%

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Fumonisin producing Fusarium spp. and fumonisin contamination in commercial South African maize

et al. 2014

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Fumonisins are secondary, carcinogenic metabolites produced primarily by Fusarium verticillioides and Fusarium proliferatum on maize worldwide. The natural occurrence of fumonisin-producing Fusarium spp. and fumonisin contamination of maize grain were quantified in selected maize cultivars from principal production areas of South Africa. Grain colonization by Fusarium spp. was determined using quantitative real-time PCR (qPCR) and contamination with fumonisins using HPLC analysis. Kernels from the 2007 samples were also plated onto Fusarium selective medium and subsequently, split plates containing PDA & CLA. The number of fumonisin producing Fusarium spp. were quantified and microscopically identified after 14 days. Simple linear regression analysis was used to determine the relationship between target DNA, fumonisins and the number of fumonisin producing Fusarium spp. using the plating out method. Results indicated high natural infection by fumonisinproducing Fusarium spp. and fumonisin concentrations in warmer production areas such as Northern Cape, North West and Free State Provinces. Spearman Ranking Correlations indicated that the responses of cultivars to colonization of grain by fumonisin producing Fusarium spp. varied over localities/seasons (r s =0.42 to 0.64) suggesting that cultivars reacted differently to different environmental/inoculum conditions (disease potentials). Cultivars CRN3505 and DKC80-12B showed a degree of resistance to fungal infection. As with fungal colonization, Spearman Rank Correlations indicated the response of cultivars to fumonisin contamination to vary over localities/ seasons (r s =0.29 to 0.70). Cultivars DKC80-12B and LS8521B showed a degree of resistance to fumonisin contamination. Regression analysis yielded a significant relationship between HPLC data and qPCR, but not with the plating out of grain data suggesting the former to be a better indicator of potential fumonisin contamination. Site-specific, daily maximum temperature and rainfall data were provided by the ARC-Institute for Soil Water and Climate's meteorology office. No significant relationship between these weather parameters and colonization of grain by fumonisin producing Fusarium spp. was recorded, although a tendency was observed between fumonisin contamination and mean maximum temperature.

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Section: Correlation Between Qpcr Hplc and The Plating Out Methodssupporting

confidence: 91%

mentioning

confidence: 98%

Fumonisin producing Fusarium spp. and fumonisin contamination in commercial South African maize

et al. 2014

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show abstract

<i>Fusarium</i> spp. and levels of fumonisins in maize produced by subsistence farmers in South Africa

Cited by 1 publication

References 14 publications

Fumonisin producing Fusarium spp. and fumonisin contamination in commercial South African maize

Fumonisin producing Fusarium spp. and fumonisin contamination in commercial South African maize

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