<i>Dendrobium officinale</i> Attenuates Myocardial Fibrosis <i>via</i> Inhibiting EMT Signaling Pathway in HFD/STZ-Induced Diabetic Mice

Abstract

Cardiac fibrosis is a major contributor for diabetic cardiomyopathy and Dendrobium officinale possessed therapeutic effects on hyperglycemia and diabetic cardiomyopathy. To further investigate the possible mechanisms of the Dendrobium officinale on diabetic myocardial fibrosis in mice. Water-soluble extracts of Dendrobium officinale (DOE) from dry stem was analyzed by HPLC and phenol-sulfuric acid method. Diabetic mice were induced by intraperitoneal injection of streptozotocin (STZ) (30 mg/kg) for 4 consecutive days after intragastric administration of a high-fat diet (HFD) for 2 weeks. The groups were as follows: control group, model group, DOE low, medium, high dose group (75, 150, 300 mg/kg) and Metformin positive group (125 mg/kg). The results showed that DOE dose-dependently lower serum insulin, total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and grew the high-density lipoprotein cholesterol (HDL-C) after 12 weeks of daily administration with DOE. Hematoxylin-eosin staining and Sirius red staining showed obvious amelioration of cardiac injury and fibrosis. In addition, the result of immunoblot indicated that DOE increased the expression of peroxisome proliferator activated receptor-α (PPAR-α), phosphorylation of insulin receptor substrate 1 (p-IRS1) and E-cadherin and repressed the expression of transforming growth factor β1 (TGF-β1), phosphorylation of c-Jun N-terminal kinase (p-JNK), Twist, Snail1 and Vimentin. The present findings suggested that DOE ameliorated HFD/STZ-induced diabetic cardiomyopathy (DCM). The possible mechanism mainly associated with DOE accelerating lipid transport, inhibiting insulin resistant and suppressing fibrosis induced by epithelial mesenchymal transition (EMT).

CYP1B1 as a therapeutic target in cardio-oncology

“…Likewise, CYP1B1-mediated formation of genotoxic metabolites and DNA adducts lead to atherosclerosis and cardiovascular disease [100,101], and may also contribute to CYP1B1-mediated carcinogenesis [134]. CYP1B1 has also been shown to induce EMT which is involved in cardiac fibrosis [399,400] and in cancer progression [126,401]. CYP1B1-mediated inflammation, which has deleterious effects on the cardiovascular system [19,85,86], can also contribute to carcinogenesis and tumor progression [126,402].…”

Traditional Uses, Phytochemistry, Pharmacology, and Quality Control of Dendrobium officinale Kimura et. Migo

“…Pharmacological results published in the literature have revealed multiple promising bioactivities of D. officinale, including cardioprotective (Xiao et al, 2018), anti-tumor (Guo et al, 2019), gastrointestinal protective (Liu et al, 2020), anti-diabetes (Zeng et al, 2020), immunomodulatory (Huang et al, 2018), anti-aging (Liang et al, 2017), and anti-osteoporosis (Wang et al, 2018) effects. Besides, it is usually prescribed as one of the ingredients of herbal formula, such as "Tin maple crystal" (Tiepifengdoujing: D. officinale and American ginseng), for regulating the gastrointestinal tract in traditional and contemporary clinical practice (Qin et al, 2019).…”

MicroRNA-195-5p Downregulation Inhibits Endothelial Mesenchymal Transition and Myocardial Fibrosis in Diabetic Cardiomyopathy by Targeting Smad7 and Inhibiting Transforming Growth Factor Beta 1-Smads-Snail Pathway

“…Photographs were captured with an optical microscope (Olympus; 200 ×). The red area of collagen deposition was counted by Image Pro Plus 6.0 (Media Cybernetics, Bethesda, MD, United States; Zeng et al, 2020 ).…”