<i>BIRC5</i>, <i>GAJ5,</i> and lncRNA NPHP3-AS1 Are Correlated with the Development of Atrial Fibrillation-Valvular Heart Disease

Abstract

The aim of this study was to explore the pivotal genes or lncRNAs involved in the progression of atrial fibrillation (AF)-valvular heart disease (VHD). The mRNA profiling GSE113013 was obtained from the Gene Expression Omnibus database. The identification of differentially expressed genes (DEGs) and differentially expressed long non-coding RNAs (DElncRNAs) was performed. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were carried out for DEGs. Then, the construction of the protein-protein interaction (PPI) network was conducted. An lncRNA-miRNA-target ceRNA network was constructed after obtaining microRNAs (miRNA) related to DElncRNAs. Ultimately, key disease-related genes were screened. A total of 399 DEGs and 145 DElncRNAs were obtained. There were 283 nodes and 588 interaction pairs in the PPI network, and synaptosome-associated protein 25 (SNAP25) had higher degrees (degree = 22) in the PPI network. There were 65 interaction pairs in the ceRNA network. Here, Baculoviral IAP Repeat Containing 5 (BIRC5) was regulated by hsa-miR-1285-3p, which was regulated by lncRNA NPHP3-AS1. Gap Junction Protein Alpha 5 (GAJ5) was regulated by hsa- miR-4505, hsa-miR-1972, and hsa-miR-1199-5p. In particular, GAJ5 was enriched in the function of ion transmembrane transport regulation, whereas BIRC5 was enriched in the function of apoptosis-multiple species pathway. Similarly, Potassium Inwardly Rectifying Channel Subfamily J Member 6 (KCNJ6) was enriched in the function of an ion channel complex. VENN analysis identified BIRC5 and GJA5 as key AF-related genes. KCNJ6, SNAP25, GJA5, BIRC5, hsa-miR-1285-3p, and lncRNA NPHP3-AS1 were likely to be associated with AF-VHD development.

Cardiac transcriptomic changes induced by early CKD in mice reveal novel pathways involved in the pathogenesis of Cardiorenal syndrome type 4

“…Still, when T cell reactivity is sustained, these reparative efforts often result in adverse remodeling that, when chronic, can progress to HF. Birc5 encodes survivin, a known apoptosis inhibitor that regulates T cell responses identified as a critical gene in the progression of atrial fibrillation [ 39 ]. Knockdown or pharmacological inhibition of Birc5 in T cells attenuates acute allograft rejection survival of cardiac allografts after murine heterotopic heart transplantation by inducing apoptosis [ 30 ].…”

High expression of long noncoding RNA plasmacytoma variant translocation 1 is an independent risk factor for recurrence after radiofrequency ablation in atrial fibrillation patients

“…LncRNAs are demonstrated to have biological functions in the pathological process of cardiovascular diseases and are differentially expressed in AF patients, such as lncRNA MIAT, lncRNA LINC00472, and lncRNA NPHP3-AS1. 12,22,23 It has been reported that lncRNA GAS5 is a potential biomarker for the diagnosis and prognosis of AF and can be used for the prognosis of AF progression and recurrence. 6 LncRNA LINC00472 is lowly expressed in AF patients; it reduces the expression of JP2 and RyR2 through miR-24 and participates in the pathogenesis of AF.…”

Identification of differentially expressed genes and signaling pathways in gestational diabetes mellitus by integrated bioinformatics analysis

“…Existing evidences have reported that HNF4G [39], SLC15A1 [40], CXCL5 [41] and MALAT1 [42] promotes obesity, but these genes might be novel target for GDM. NPHP3-AS1 [43], LTA (lymphotoxin alpha) [44], CXCL5 [45] and MALAT1 [46] are found to be expressed in cardiovascular diseases, but these genes might be novel targets for GDM. A recent study demonstrated that XIST (X inactive specific transcript) [47] and MALAT1 [48] serves a crucial role in GDM progression.…”