&lt;i&gt;Aeromonas veronii&lt;/i&gt; Infection Associated with High Morbidity and Mortality in Farmed European Seabass &lt;i&gt;Dicentrarchus labrax&lt;/i&gt; in the Aegean Sea, Greece

Smyrli, Maria; Prapas, Athanasios; Rigos, George; Kokkari, Constantina; Pavlidis, M.; Katharios, Pantelis

doi:10.3147/jsfp.52.68

Cited by 35 publications

(39 citation statements)

References 40 publications

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“…Localities included in the study were selected based on reports from the fish vets of the affected farms for aeromonad-suspected morbidity and mortality. Extended sampling was conducted in the area of Argolikos Bay where the disease was first described [9]. Microbial screenings on diseased fish farmed in sea-cages, were conducted at various times (2009–2019) including periods of disease outbreaks but also periods when fish showed no signs of the disease.…”

Section: Methodsmentioning

confidence: 99%

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Comparative Study on A Novel Pathogen of European Seabass. Diversity of Aeromonas veronii in the Aegean Sea

Smyrli

Triga

Dourala³

et al. 2019

Microorganisms

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Aeromonas veronii is an emerging pathogen causing severe pathology and mortalities in European seabass aquaculture in the Aegean Sea, Mediterranean. More than 50 strains of the pathogen were characterized biochemically and genetically in order to study the epidemiology of the disease, as well as the phylogeny and virulence of the bacterium. Based on the phenotypic characteristics, the isolates form three groups consisting of: (a) the West Aegean Sea, non-motile, non-pigment-producing strains, (b) the West Aegean Sea, motile, and pigment-producing strains and (c) the East Aegean Sea motile strains that produce minute amounts of pigment. All strains were highly similar at the genomic level; however, the pattern of West/East geographic origin was reflected in biochemical properties, in general genomic level comparison and in the putative virulent factors studied. Type VI secretion system was not detected in the western strains. The outer membrane protein (OMP) profile which contains proteins that are putative antigenic factors, was very similar between strains from the different areas. Although most of the OMPs were detected in all strains with great sequence similarity, diversification according to geographic origin was evident in known antigenic factors such as the maltoporin LamB. A systematic comparative analysis of the strains is presented and discussed in view of the emergence of A. veronii as a significant pathogen for the Mediterranean aquaculture.

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Section: Methodsmentioning

confidence: 99%

“…sobria has become extremely problematic in the culture of European seabass ( Dicentrarchus labrax ) in Greece. The disease first appeared in 2008 affecting a single fish farm in Central Greece [9]. While it is still present in the specific farm, more farms in distant areas are also affected.…”

Section: Introductionmentioning

confidence: 99%

Comparative Study on A Novel Pathogen of European Seabass. Diversity of Aeromonas veronii in the Aegean Sea

Smyrli

Triga

Dourala³

et al. 2019

Microorganisms

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“…Members of the genus possess specific proteins and enzymes that can facilitate both attachment to chitinous surface and degradation of crustacean's exoskeleton to obtain nutrients [46]. Aeromonads have also the same characteristics and the most notable species, Aeromonas hydrophila and A. veronii [47], are both important pathogens of European sea bass [48]. We do not know the species that epibiotic bacterial biofilm covering C. oestroides body parts belongs to, or whether it poses a potential threat to the fish host.…”

Section: Discussionmentioning

confidence: 99%

Host-Parasite Interaction between Parasitic Cymothoid Ceratothoa oestroides and Its Host, Farmed European Sea Bass (Dicentrarchus labrax)

et al. 2020

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Parasitic isopod Ceratothoa oestroides (Cymothoidea, Isopoda) is a common and generalist buccal cavity-dweller in marine fish, recognised for its detrimental effect in fingerling and juvenile farmed European sea bass (Dicentrarchus labrax). Although distributed throughout the Mediterranean, the isopod provokes acute outbreaks mainly limited to particular endemic areas in Croatia (Adriatic Sea) and Greece (Aegean Sea). While numerous studies have previously evidenced its gross effect on farmed fish (i.e. decreased condition index, slower growth rate, lethargy and mortality), details on the host-parasite interaction are still lacking. Therefore, using a multimethodological approach, we closely examined the structure and appearance of isopod body parts acting in the attachment and feeding (stereomicroscopy, scanning and transmission electron microscopy), and the extent of host tissues damage (histology, immunohistochemistry, micro-computational tomography) induced by parasitation. Interestingly, while hematophagous nature of the parasite has been previously postulated we found no unambiguous data to support this; we observed host tissues fragmentation and extensive hyperplasia at the parasitation site, and no structures indicative of heme detoxifying mechanisms in the parasite gut, or other traces of a blood meal. The bacterial biofilm covering C. oestroides mouthparts and pereopods suggests that the isopod may play a role in conveying secondary pathogens to the infected host, or alternatively, it serves the parasite in normal interaction with its environment.

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“…The four strains of Aeromonas veronii bv. sobria (NS2, NS22, PDB and NS13) were isolated from European seabass (Dicentrarchus labrax) [60], Edwardsiella anguillarum (EA011113) was isolated from sharpsnout seabream (Diplodus puntazzo) [61,62], Vibrio alginolyticus (V1) from gilthead seabream (Sparus aurata) [63], Vibrio harveyi (VhP1) and Vibrio anguillarum (Vak) from European seabass and Photobacterium damselae subsp. piscicida (PDKef) from common seabream (Pagrus pagrus).…”

Section: Evaluation Of Anti-bacterial Activitymentioning

confidence: 99%

Comparative Evaluation of Essential Oils from Medicinal-Aromatic Plants of Greece: Chemical Composition, Antioxidant Capacity and Antimicrobial Activity against Bacterial Fish Pathogens

Anastasiou

Mandalakis

Krigas³

et al. 2019

Molecules

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The administration of antibiotics in aquaculture has raised concern about the impact of their overuse in marine ecosystems, seafood safety and consumers’ health. This “green consumerism” has forced researchers to find new alternatives against fish pathogens. The present study focused on 12 Mediterranean medicinal-aromatic plants as potential antimicrobials and antioxidant agents that could be used in fish aquaculture. In vitro assays showed that the essential oils (EOs) from all studied plants had anti-bacterial and antioxidant properties, with their efficacy being dependent on their chemical composition. More specifically, EOs rich in carvacrol, p-cymene and γ-terpinene exhibited not only the strongest inhibitory activity against the growth of bacterial pathogens (inhibitory concentration: 26–88 μg mL−1), but also the greatest total antioxidant capacity (ABTS: 2591–5879 μmole mL−1; CUPRAC: 931–2733 μmole mL−1). These compounds were mainly found in the EOs from Greek oregano (Origanum vulgare subsp. hirtum), Spanish oregano (Thymbra capitata) and savoury (Satureja thymbra) collected from cultivations in Greece. The specific EOs stand out as promising candidates for the treatment of bacterial diseases and oxidative stress in farmed fish. Further in vivo experiments are needed to fully understand the effects of EO dietary supplementation on fish farming processes.

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<i>Aeromonas veronii</i> Infection Associated with High Morbidity and Mortality in Farmed European Seabass <i>Dicentrarchus labrax</i> in the Aegean Sea, Greece

Cited by 35 publications

References 40 publications

Comparative Study on A Novel Pathogen of European Seabass. Diversity of Aeromonas veronii in the Aegean Sea

Comparative Study on A Novel Pathogen of European Seabass. Diversity of Aeromonas veronii in the Aegean Sea

Host-Parasite Interaction between Parasitic Cymothoid Ceratothoa oestroides and Its Host, Farmed European Sea Bass (Dicentrarchus labrax)

Comparative Evaluation of Essential Oils from Medicinal-Aromatic Plants of Greece: Chemical Composition, Antioxidant Capacity and Antimicrobial Activity against Bacterial Fish Pathogens

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