&lt;i&gt;Acinetobacter lwoffii&lt;/i&gt; An Emerging Pathogen in Neonatal ICU

Mittal, Sakshi; Sharma, Madhu; Yadav, Akhilesh Kumar; Bala, Kiran; Chaudhary, Uma

doi:10.2174/1871526515666150826114745

Cited by 23 publications

(16 citation statements)

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“…These bacteria are not considered priority pathogens on WHO's published list of antibiotic–resistant “priority pathogens” (Lawe-Davis and Bennett, 2017). Despite this, it should be emphasized that A. Iwoffii , which is ubiquitous in the environment is an emerging multidrug resistant pathogen in neonatal sepsis (Mittal et al, 2015). Furthermore, ARGs encoding for resistance against cephalosporins, macrolides, polymyxins, quinolones, and aminoglycosides, that are categorized as critically important antimicrobials for human medicine by WHO (2016), persisted throughout the wastewater treatment process.…”

Section: Discussionmentioning

confidence: 99%

Metagenomic and Resistome Analysis of a Full-Scale Municipal Wastewater Treatment Plant in Singapore Containing Membrane Bioreactors

Tan

Jiang

et al. 2019

Front. Microbiol.

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Reclaimed water provides a water supply alternative to address problems of scarcity in urbanized cities with high living densities and limited natural water resources. In this study, wastewater metagenomes from 6 stages of a wastewater treatment plant (WWTP) integrating conventional and membrane bioreactor (MBR) treatment were evaluated for diversity of antibiotic resistance genes (ARGs) and bacteria, and relative abundance of class 1 integron integrases ( intl1 ). ARGs confering resistance to 12 classes of antibiotics (ARG types) persisted through the treatment stages, which included genes that confer resistance to aminoglycoside [aadA, aph(6)-I, aph(3 ′ )-I, aac(6 ′ )-I, aac(6 ′ )-II, ant(2 ″ )-I] , beta-lactams [class A, class C, class D beta-lactamases ( bla OXA )], chloramphenicol (acetyltransferase, exporters, floR, cmIA ), fosmidomycin ( rosAB ), macrolide-lincosamide-streptogramin ( macAB, ereA, ermFB ), multidrug resistance (subunits of transporters), polymyxin ( arnA ), quinolone ( qnrS ), rifamycin ( arr ), sulfonamide ( sul1, sul2 ), and tetracycline ( tetM, tetG, tetE, tet36, tet39, tetR, tet43, tetQ, tetX ). Although the ARG subtypes in sludge and MBR effluents reduced in diversity relative to the influent, clinically relevant beta lactamases (i.e., bla KPC , bla OXA ) were detected, casting light on other potential point sources of ARG dissemination within the wastewater treatment process. To gain a deeper insight into the types of bacteria that may survive the MBR removal process, genome bins were recovered from metagenomic data of MBR effluents. A total of 101 close to complete draft genomes were assembled and annotated to reveal a variety of bacteria bearing metal resistance genes and ARGs in the MBR effluent. Three bins in particular were affiliated to Mycobacterium smegmatis, Acinetobacter Iwoffii , and Flavobacterium psychrophila , and carried aquired ARGs aac(2 ′ )-Ib, bla OXA−278 , and tet36 respectively. In terms of indicator organisms, cumulative log removal values (LRV) of Escherichia coli, Enterococci , and P. aeruginosa from influent to conventional treated effluent was lower (0–2.4), compared to MBR effluent (5.3–7.4). We conclude that MBR is an effective treatment method for reducing fecal indicators and ARGs; however, incomplete removal of P. aeruginosa in MBR treated effluents (<8 MPN/100 mL) and the presence of ARGs and intl1 underscores...

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Section: Discussionmentioning

confidence: 99%

Metagenomic and Resistome Analysis of a Full-Scale Municipal Wastewater Treatment Plant in Singapore Containing Membrane Bioreactors

Tan

Jiang

et al. 2019

Front. Microbiol.

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“…Thus far, no C. elegans in vivo-studies concerning these two bacteria have been reported in the literature, but their potential pathogeny for humans is well known. Bacteremia and infections caused by A. iwoffii and M. luteus are mostly registered as healthcare-associated infections [69][70][71][72]. In comparison with other microorganisms, the two isolates showed certain pathogenic behaviors because they caused all the typical symptoms and phenotypes of a C. elegans infection, as reported by Ewbank and Pujol [73].…”

Section: In Vivo Assaymentioning

confidence: 79%

Evaluation of Bioaerosol Bacterial Components of a Wastewater Treatment Plant Through an Integrate Approach and In Vivo Assessment

Bruni

Simonetti

Bovone

et al. 2019

IJERPH

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Wastewater carries different pathogenic and non-pathogenic microorganisms that can be dispersed in the surrounding environment. Workers who frequent sewage treatment plants can therefore be exposed to aerosols that contain a high concentration of potentially dangerous biological agents, or they can come into direct contact with contaminated material. This can lead to allergies, infections and occupational health-associated diseases. A characterization of biological risk assessment of bioaerosol exposure is necessary. The aim of this study was to evaluate the application of an interdisciplinary method that combines chemical and biological approaches for the analysis of a bioaerosol derived from a wastewater treatment plant (WWTP) situated in Italy. Sampled filters were analyzed by HPLC-MS/MS spectroscopy that searched for different chemical biomarkers of airborne microorganisms. The analytical quantification was compared to the biological cultural method that revealed an underrated microbial concentration. Furthermore, next generation sequencing analysis was used also to identify the uncultivable species that were not detected by the culture dependent-method. Moreover, the simple animal model Caenorhabditis elegans was used to evaluate the pathogenicity of two isolates—Acinetobacter iwoffii and Micrococcus luteus—that showed multidrug-resistance. This work represents a starting point for the development of a multidisciplinary approach for the validation of bioaerosol exposure on WWTP workplaces.

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“…Among the more frequent non- baumannii Acinetobacter species causing infections, we found A. junii, A. soli, A. ursingii, A. nosocomialis and A. lwoffii [3] , [4] , [5] . However, A. schindleri, which was first described in isolates recovered mostly from nonsterile body sites of outpatients, has not been reported in clinically significant isolates from seriously ill hospitalized patients.…”

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confidence: 79%

First case of bacteraemia due to Acinetobacter schindleri harbouring bla NDM-1 in an immunocompromised patient

Montaña

Palombarani²,

Carulla³

et al. 2018

New Microbes and New Infections

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<i>Acinetobacter lwoffii</i> An Emerging Pathogen in Neonatal ICU

Cited by 23 publications

References 0 publications

Metagenomic and Resistome Analysis of a Full-Scale Municipal Wastewater Treatment Plant in Singapore Containing Membrane Bioreactors

Metagenomic and Resistome Analysis of a Full-Scale Municipal Wastewater Treatment Plant in Singapore Containing Membrane Bioreactors

Evaluation of Bioaerosol Bacterial Components of a Wastewater Treatment Plant Through an Integrate Approach and In Vivo Assessment

First case of bacteraemia due to Acinetobacter schindleri harbouring bla NDM-1 in an immunocompromised patient

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