&lt;em&gt;NT5DC2&lt;/em&gt; is a novel prognostic marker in human hepatocellular carcinoma

Chen, Jiemin; Cao, Jianzhong; Wang, Peng-Hui; He, Xiaodong

doi:10.3892/ol.2020.11931

Cited by 10 publications

(12 citation statements)

References 46 publications

(54 reference statements)

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“…In addition, we observed a positive correlation of NT5DC2 and p53 protein and gene expression in both cohorts and depicted a p53 dependent NT5DC2 effect, warranting further investigation in future studies. Because NT5DC2 is a cytoplasmatic protein, which can be found not only in NSCLC but also in leiomyosarcoma [21], colorectal cancer [20] and hepatocellular carcinoma [23], therapeutic interactions are conceivable for multiple entities, and its interaction with p53 makes it a valuable target for further investigation. In particular, the impact of NT5DC2 with respect to p53 mutations, isoforms and deficiency itself and their effect on tumor stroma and on CAFs should further be investigated to determine whether it adversely affects the outcome of NSCLC course.…”

Section: Discussionmentioning

confidence: 99%

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High Expression of NT5DC2 Is a Negative Prognostic Marker in Pulmonary Adenocarcinoma

Schulze

Schmidt

Möhr

et al. 2022

Cancers

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Via immunohistochemistry (IHC) on tissue micro arrays (TMA) clinical and prognostic impact of p53 co-playing 5′-Nucleotidase Domain-Containing Protein 2 (NT5DC2) protein expression was evaluated in 252 NSCLC patients. Confirmatory, gene expression database. mRNA levels of NT5DC2 were studied in 1925 NSCLC patients. High protein expression of NT5DC2 resulted in reduced median overall survival (OS) of patients with stage I-III adenocarcinoma (ADC) (Log Rank p = 0.026, HR 2.04 (1.08–3.87)), but not in squamous cell carcinoma (SCC) (p = 0.514, HR 0.87 (0.57–1.33)). Findings on OS were reproduced via gene expression analysis in ADC (p < 0.001, HR 1.64 (1.30–2.08)) and SCC (p = 0.217, HR 0.86 (0.68–1.09)). Yet, NT5DC2 mRNA levels were higher in SCC compared to ADC (p < 0.001) and in pN2 tumors compared to pN0/1 tumors (p = 0.001). Likewise, NT5DC2 protein expression associated with high-grade SCC. Moreover, NT5DC2 expression was positively correlated with p53 protein (p = 0.018) and TP53 gene expression (p < 0.001) and its survival effect was p53 dependent. While p53 expression was negatively associated with the presence of CD34+ cancer associated fibroblasts (CAFs), NT5DC2 expression insignificantly tended to higher levels of SMA+ CAFs (p = 0.065).

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Section: Discussionmentioning

confidence: 99%

“…In addition, the impact of NT5DC2 on tumorigenesis and progression was also investigated in other entities. Here, knockdown of NT5DC2 resulted in limited progression of colorectal cancer cell models [20], whereas overexpression of NT5DC2 enhanced tumor growth in leiomyosarcoma [21], glioma [22] and hepatocellular carcinoma [23].…”

Section: Introductionmentioning

confidence: 92%

High Expression of NT5DC2 Is a Negative Prognostic Marker in Pulmonary Adenocarcinoma

Schulze

Schmidt

Möhr

et al. 2022

Cancers

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“…Due to the insidiousness of this disease, patients are often diagnosed with advanced HCC at their initial examination in the hospital, resulting in a poor prognosis (35). At present, chemotherapy and immunotherapy are comparatively the best options for the treatment of HCC (36). Therefore, it is particularly important to perform more in-depth research on the pathogenesis of HCC, with the ASPM is highly expressed in various types of cancer, including breast (13), bladder (12) and ovarian cancer (16).…”

Section: Discussionmentioning

confidence: 99%

ASPM combined with KIF11 promotes the malignant progression of hepatocellular carcinoma via the Wnt/β‑catenin signaling pathway

Chun-yang

Kong

2021

Exp Ther Med

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To investigate the molecular mechanism of assembly factor for spindle microtubules (ASPM) in the regulation of the malignant progression of hepatocellular carcinoma (HCC), bioinformatics analysis was utilized to analyze the role of ASPM in the malignant progression of HCC and its potential interaction with the kinesin family member 11 (KIF11) gene. The expression levels of ASPM and KIF11 were detected by reverse transcription-quantitative PCR and western blotting. Following knockdown of ASPM expression, Cell Counting Kit-8/colony formation assays were performed to detect cell viability and proliferation. Wound healing and Transwell assays were employed to detect cell migration and invasion. Additionally, a co-immunoprecipitation (CO-IP) assay was used to detect whether there was an interaction between ASPM and KIF11. KIF11 overexpression was performed to verify if ASPM exerted its effects via KIF11. ASPM was highly expressed in HCC tissues and cells, and was closely associated with a poor prognosis of patients with HCC. Interference with ASPM expression markedly inhibited the viability, proliferation, invasion and migration of HCC cells. Using a CO-IP assay, it was revealed that there was an interaction between ASPM and KIF11. Rescue experiments subsequently revealed the regulatory effects of ASPM on the activity, proliferation, invasion and migration of HCC cells via KIF11. Finally, western blot analysis demonstrated that ASPM in combination with KIF11 promoted the malignant progression of HCC by regulating the activity of the Wnt/β-catenin signaling pathway. Therefore, the present study demonstrated that ASPM may interact with KIF11 in HCC cells to promote the malignant progression of HCC via the Wnt/β-catenin signaling pathway.

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“…Currently, only a few studies have investigated the association between NT5DC family members and HCC. Chen et al found that NT5DC2 was highly expressed in liver cancer tissues, and the overexpression of NT5DC2 indicated poor overall survival (OS) and relapse-free survival (RFS) [ 10 ]. Li et al showed the overexpression of NT5DC2 promoted liver cancer cell proliferation through stabilizing epidermal growth factor receptor (EGFR) [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of Prognostic Value and Immune Infiltration of the NT5DC Family in Hepatocellular Carcinoma

Liu

Zheng

et al. 2022

Journal of Oncology

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Background. Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in the world, and its incidence is obviously increasing. The NT5DC family has been shown to be involved in the progression of many tumors. However, the biological function of NT5DC family members in HCC is still not well understood. Methods. Oncomine, Gene Expression Profiling Interactive Analysis (GEPIA), UALCAN, Kaplan–Meier plotter, cBioPortal, GeneMANIA, Metascape, and TIMER were applied to assess the biological function of NT5DC family members in HCC. Results. Most of the NT5DC family members were highly expressed in HCC. High expression of NT5C2, NT5DC2, and NT5DC3 was closely associated with higher tumor stage and poor overall survival (OS). In addition, high NT5DC2 and NT5DC3 expression also predicted poor disease-free survival (DFS). Enrichment analysis revealed that the NT5DC family in HCC mainly involved the IMP metabolic process, purine ribonucleoside monophosphate metabolic process, and purine nucleoside monophosphate metabolic process. The expression of NT5DC family members was closely related to the infiltration of some immune cells, such as B cells, CD8+ T cells, CD4+ T cells, macrophages, neutrophils, and dendritic cells. Conclusion. Our findings provided new insights into the biological function and prognostic value of NT5DC family members in HCC.

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<em>NT5DC2</em> is a novel prognostic marker in human hepatocellular carcinoma

Cited by 10 publications

References 46 publications

High Expression of NT5DC2 Is a Negative Prognostic Marker in Pulmonary Adenocarcinoma

High Expression of NT5DC2 Is a Negative Prognostic Marker in Pulmonary Adenocarcinoma

ASPM combined with KIF11 promotes the malignant progression of hepatocellular carcinoma via the Wnt/β‑catenin signaling pathway

Comprehensive Analysis of Prognostic Value and Immune Infiltration of the NT5DC Family in Hepatocellular Carcinoma

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