<em>let-7d</em> suppresses proliferation and invasion and promotes apoptosis of meningioma by targeting AEG-1

Abstract

Backgroundlet-7d has been indicated to act as a tumor suppressor in various cancers. However, the function and molecular mechanism of let-7d in meningioma progression have not been elucidated.Materials and methodsQuantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the expression levels of let-7d and AEG-1 mRNA in meningioma tissues and cell lines. The protein level of AEG-1 was measured by Western blot analysis. MTT assay, Transwell invasion assay and flow cytometry analysis were carried out to determine the proliferation, invasion and apoptosis of IOMM-Lee and CH-157MN cells, respectively. Target gene of let-7d was verified by luciferase reporter analysis.Resultslet-7d expression was downregulated, and AEG-1 expression was upregulated in meningioma tumor tissues. let-7d overexpression suppressed proliferation and invasion and induced apoptosis in IOMM-Lee and CH-157MN cells. Moreover, AEG-1 was a direct target of let-7d. Restoration of AEG-1 expression reversed let-7d-mediated suppression of the proliferation and invasion and let-7d-induced apoptosis in IOMM-Lee and CH-157MN cells.Conclusionlet-7d repressed proliferation and invasion and promoted apoptosis of meningioma cells by targeting AEG-1. The present study provided a better understanding of the meningioma pathogenesis and a promising therapeutic target for meningioma patients.

Up‐regulation of KISS1 as a novel target of Let‐7i in melanoma serves as a potential suppressor of migration and proliferation in vitro

“… 27 , 28 , 31 Besides, Let‐7i was reported as a potential tumour suppressor microRNA in melanoma by reducing cell viability in human malignant melanoma cells in vitro by decreasing either cell proliferation or apoptosis induction. 32 , 33 , 34 All of these studies were consistent with the present study in which showed that restoration of Let‐7i could reduce cell proliferation, migration as well as induced apoptosis in melanoma cells in vitro.…”

Molecular Mechanism and Approach in Progression of Meningioma

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A Clinical Update on the Prognostic Effect of microRNA Biomarkers for Survival Outcome in Nasopharyngeal Carcinoma: A Systematic Review and Meta-Analysis

“…Let-7d expression was found to be low in HNC patients, which was linked to a poor prognosis. As a result, let-7d inhibits tumorigenesis and promotes apoptosis in tumour cells via AEG-1 [62].…”