&lt;em&gt;In-vitro&lt;/em&gt; establishment, germination and growth performance of Red Sandalwood (&lt;em&gt;Pterocarpus santalinus&lt;/em&gt; L.)

Chaturani, G. D. G.; Subasinghe, S.; Jayatilleke, M. P.

doi:10.4038/tare.v9i0.5411

Cited by 12 publications

(2 citation statements)

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“…In addition, the uniform and synchronous suspension cultures make them suitable for the creation of phytochemicals at a large scale. Substantial work has been done in sandalwood by several scientists throughout the world so far by employing an array of explants for inducing morphogenesis followed by plantlet regeneration with varying level of accomplishment; for instance, embryos [10,14], hypocotyls [14][15][16], shoot tips [17,18], nodal segments [19][20][21][22], leaf discs [23,24], seedlings [25], endosperms [15,16], cotyledons [14] and protoplasts [26] have been used jointly with cell suspension cultures [11][12][13]27].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Different Factors for Initiation of Somatic Embryogenesis in Suspension Cultures in Sandalwood (Santalum album L.)

Tripathi¹,

Tripathi

Tiwari³

et al. 2021

Horticulturae

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Santalum album (L.) is a prized tropical tree species of high therapeutic and industrial importance. The wood of these naturally grown plants is extensively harvested to acquire therapeutically important metabolite santalol and be used for additional functions such as in wood statuette industries. Due to high demand, it is crucial to maintain a sufficient plant population. An easy protocol for establishing cell suspension culture initiated from the loose embryogenic callus mass of sandalwood was realized by shifting 6–8-week-old morphogenic calli acquired from the mature embryonic axis and cotyledon explant cultures in fluid media. The asynchronous embryogenic cultures were sloughed with clumps of flourishing cell clumps and embryos of various progressive phases along with diffident non-embryogenic tissues. The frequency of embryo proliferation was evidenced to determinethe expansion pace of embryogenic masses under diverse conditions. The intonation of initiation and creation of cell suspension was under the directive of the influence of exogenous plant growth regulators amended in the nutrient medium at different concentrations and combinations. Maximum relative growth rate (386%) and clumps/embryoids in elevated integers (321.44) were accomplished on MS nutrient medium fortified with 2.0 mg L−1 2,4-D in association with 0.5 mg L−1 BA and 30.0 g L−1 sucrose raised from mature embryonic axis-derived calli. Plantlet regeneration in higher frequency (84.43%) was evidenced on MS medium amended with 1.0 mg L−1 each of TDZ and GA3 in conjunction with 0.5 mg L−1 NAA and 20.0 g L−1 sucrose. Mature embryonic axis-derived calli were found to be constantly better than mature cotyledon-derived calli for raising profitable and reproducible cell suspension cultures. Regenerants displayed normal growth and morphology and were founded successfully in the external environment after hardening.

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Section: Introductionmentioning

confidence: 99%

Optimization of Different Factors for Initiation of Somatic Embryogenesis in Suspension Cultures in Sandalwood (Santalum album L.)

Tripathi¹,

Tripathi

Tiwari³

et al. 2021

Horticulturae

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“…The natural regeneration of the plant is di cult due to hard pod, low fruit formation, stringent habitat, lengthened dormancy, and impoverished seed development (Rao and Raju 2002;Hegde et al 2012; Arunkumar and Joshi 2014). Conventional regeneration methods like grafting, root cutting, and air layering proved futile (Chaturani 2006; Vijayalakshmi and Renganayaki 2017; Renganayaki et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis and plantlet regeneration in red sandalwood (Pterocarpus santalinus)

Chakraborty

Chaitanya

Akhtar

2023

Plant Cell Tiss Organ Cult

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Cotyledonary segments from the germinated immature zygotic embryos were used for somatic embryogenesis of red sandalwood (Pterocarpus santalinus). It was established on Murashige and Skoog (MS) medium containing 5% sucrose and amalgamation of 6-benzylaminopurine (BAP), 2,4-Dichlorophenoxyacetic acid (2,4-D), and α-Naphthaleneacetic acid (NAA). All treatments were responsive for callus induction with the frequency range between 36-97%. The maximum embryogenic frequency (69.44%) was obtained when 0.1 mg/l BAP + 2 mg/l 2,4-D and 0.1 mg/l BAP + 4 mg/l 2,4-D combinations were used. When explants were treated individually with growth regulators, the maximum embryogenic frequency (58.33%) was produced by 4 mg/l 2,4-D. BAP was completely ineffective for somatic embryogenesis when used individually. The average number of globular-staged somatic embryos ranged between 1-5 (irrespective of the treatments). The maximum number of the cotyledonary-staged somatic embryos (2.85) were obtained with treatment 0.1 mg/l BAP and 2 mg/l 2,4-D. The maximum plantlets were developed (1.30) when the cotyledonary-staged embryos from 0.1 mg/l BAP and 2 mg/l 2,4-D were transferred to MS basal medium. The plantlets obtained were acclimatized and showed 100% survival in the greenhouse condition. The embryonic cells have been histologically distinguished from nonembryonic cells with dense cytoplasm and a long suspensor. The induction, maturation and germination of somatic embryos were challenging, suggesting the need for molecular approaches through proteomic expression for mass production and understanding the evolution, structure, and genetic organization of the plant species. Key Message 2,4-D and NAA, either individually or in combination with BAP has a signi cant effect on embryonic potential of cotyledon explant of P. santalinus.

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Threats and Conservation

Begum

Kumar

Divakara

2019

Red Sanders: Silviculture and Conservation

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<em>In-vitro</em> establishment, germination and growth performance of Red Sandalwood (<em>Pterocarpus santalinus</em> L.)

Cited by 12 publications

References 0 publications

Optimization of Different Factors for Initiation of Somatic Embryogenesis in Suspension Cultures in Sandalwood (Santalum album L.)

Optimization of Different Factors for Initiation of Somatic Embryogenesis in Suspension Cultures in Sandalwood (Santalum album L.)

Somatic embryogenesis and plantlet regeneration in red sandalwood (Pterocarpus santalinus)

Threats and Conservation

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