&lt;em&gt;In Utero&lt;/em&gt; Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity

Briz, Carlos G.; Navarrete, Marta; Esteban, José A.; Nieto, Marta

doi:10.3791/55139-v

Cited by 1 publication

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“…13770) ( 68 ). IUE was performed as previously described ( 69 ). Briefly, pregnant mice were anesthetized with isoflurane/oxygen, and a solution containing the specified plasmids at a concentration of 1 μg/μl was injected into the embryo’s lateral ventricle using a 30-mm pulled glass micropipette before five voltage pulses (36 mV, 50 ms) were applied using external paddles oriented to target S1.…”

Section: Methodsmentioning

confidence: 99%

Early cortical GABAergic interneurons determine the projection patterns of L4 excitatory neurons

Bragg-Gonzalo,

Aguilera,

González-Arias

et al. 2024

Sci. Adv.

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During cerebral cortex development, excitatory pyramidal neurons (PNs) establish specific projection patterns while receiving inputs from GABAergic inhibitory interneurons (INs). Whether these inhibitory inputs can shape PNs’ projection patterns is, however, unknown. While layer 4 (L4) PNs of the primary somatosensory (S1) cortex are all born as long-range callosal projection neurons (CPNs), most of them acquire local connectivity upon activity-dependent elimination of their interhemispheric axons during postnatal development. Here, we demonstrate that precise developmental regulation of inhibition is key for the retraction of S1L4 PNs’ callosal projections. Ablation of somatostatin INs leads to premature inhibition from parvalbumin INs onto S1L4 PNs and prevents them from acquiring their barrel-restricted local connectivity pattern. As a result, adult S1L4 PNs retain interhemispheric projections responding to tactile stimuli, and the mice lose whisker-based texture discrimination. Overall, we show that temporally ordered IN activity during development is key to shaping local ipsilateral S1L4 PNs’ projection pattern, which is required for fine somatosensory processing.

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Section: Methodsmentioning

confidence: 99%