2014
DOI: 10.4025/actasciagron.v37i1.18123
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<b>The effects of storage temperature on the quality and phenolic metabolism of whole and minimally processed kale leaves

Abstract: ABSTRACT. We studied the effects of storage temperature on the activities of phenylalanine ammonialyase (PAL), peroxidase (POD) and polyphenol oxidase (PPO) in minimally processed kale (Brassica oleracea var. acephala) that was stored for 15 and 9 days at 5 ± 1ºC and 10 ± 1ºC, respectively. The main visual evidence for quality loss in whole leaves was yellowing and loss of turgescence. Minimally processed leaves presented significant browning, indicating increased POD and PPO activities. The PAL activity in mi… Show more

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Cited by 29 publications
(15 citation statements)
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References 24 publications
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“…The assay for POD was conducted in accordance with Simões, Moreira, Mosquim, Soares, and Puschmann (), with 100 μl of supernatant added to a reaction medium containing 1 ml of potassium phosphate buffer (0.2 M, pH 6.0), 100 μl of guaiacol (40 mM) and 100 μl of hydrogen peroxide (23 mM), previously maintained at 25ºC in a dry bath. Absorbances were obtained using a spectrophotometer at 470 nm, at a temperature of 25ºC, for 3 min, with a 30‐s interval between readings.…”
Section: Methodsmentioning
confidence: 99%
“…The assay for POD was conducted in accordance with Simões, Moreira, Mosquim, Soares, and Puschmann (), with 100 μl of supernatant added to a reaction medium containing 1 ml of potassium phosphate buffer (0.2 M, pH 6.0), 100 μl of guaiacol (40 mM) and 100 μl of hydrogen peroxide (23 mM), previously maintained at 25ºC in a dry bath. Absorbances were obtained using a spectrophotometer at 470 nm, at a temperature of 25ºC, for 3 min, with a 30‐s interval between readings.…”
Section: Methodsmentioning
confidence: 99%
“…In lettuce, it seems that the increase in PPO activity is not due to injury, unlike POD activity . Conversely, in cabbage leaves, PPO activity increases soon after injury, while POD activity remains unchanged . It is also reported that physical trauma may induce de novo synthesis of PPO in lettuce …”
Section: Discussionmentioning
confidence: 99%
“…PPO and POD activities were determined as detailed adapted by Simões et al . A 0.25 g sample of external (0–5 mm) or internal (5–10 mm) tissue was homogenized in 6 mL of 0.2 mol L −1 sodium phosphate buffer (pH 6.0) precooled to 4 °C.…”
Section: Methodsmentioning
confidence: 99%
“…c) Extraction and determination of polyphenoloxidase activity (PPO, EC 1.14.18.1) were carried out as described by Silva (1981) and adapted by Simões et al (2015). A sample of 0.25 g with a height of 1,000 mm, 1000 mm width, and 5 mm thickness from the surface tissue located on the equatorial side of the yam slice.…”
Section: Methodsmentioning
confidence: 99%