Abstract:The present study aimed to evaluate the activation characteristics of the lebranche mullet spermatozoa in natura and diluted with CF-HBSS for 96h at 4±2°C. The semen was collected from eight wild fish in Florianópolis -SC (Brazil) (27°S) in May, during reproductive migration. Three pools of semen were divided into two treatments: in natura and diluted with CF-HBSS 1:3. The semen was activated with seawater (salinity of 34, pH 8.7 and 4±2°C) to determine: motility time, motility rate and sperm cell membrane int… Show more
“…Trachelyopterus galeatus sperm remain continuously mobile after collection. And the incorporation of other substances into the thinner, such as antioxidants, amino acids, organic acids, cryoprotectants and others, can improve storage time and the performance of sperm during cold storage (Magnotti, de Castro, et al, 2018).…”
Conservation of fish gametes is critical to increasing the success of induced reproduction and is widely used in aquaculture farming . Refrigeration at temperatures close to 4°C is one of the techniques commonly adopted to preserve semen for short periods, to keep semen quality feasible for hours, days and even weeks, maintaining its fertilization capacity (Contreras et al., 2019;Shaliutina et al., 2013). For this to occur, it is necessary to use diluting solutions that provide a protective medium, while both nourishing and reducing cell metabolism of sperm during storage conditions (Silva et al., 2018).Studies with fish have demonstrated the positive effects of refrigeration associated with the use of cryoprotectants among which
“…Trachelyopterus galeatus sperm remain continuously mobile after collection. And the incorporation of other substances into the thinner, such as antioxidants, amino acids, organic acids, cryoprotectants and others, can improve storage time and the performance of sperm during cold storage (Magnotti, de Castro, et al, 2018).…”
Conservation of fish gametes is critical to increasing the success of induced reproduction and is widely used in aquaculture farming . Refrigeration at temperatures close to 4°C is one of the techniques commonly adopted to preserve semen for short periods, to keep semen quality feasible for hours, days and even weeks, maintaining its fertilization capacity (Contreras et al., 2019;Shaliutina et al., 2013). For this to occur, it is necessary to use diluting solutions that provide a protective medium, while both nourishing and reducing cell metabolism of sperm during storage conditions (Silva et al., 2018).Studies with fish have demonstrated the positive effects of refrigeration associated with the use of cryoprotectants among which
“…Sperm motility was activated with oceanic water at salinity of 36, pH 8.55 and temperature of 20.7°C (Magnotti, Castro, et al, ; Magnotti, Figueroa, et al, ). Immediately after activation, a single observer performed optical microscopy (ICC50 HD, Leica Microsystems; 200×) in random fields of each slide without light.…”
Section: Methodsmentioning
confidence: 99%
“…Livers and gonads were sampled (n = 3 per tank, n = 9 per treatment) to determine gonadosomatic index (GSI) and hepatosomatic index (HSI) (Vazzoler, 1982) Fish were anaesthetized with benzocaine (50 mg/L) in the urogenital region and the anal fins were cleaned and dried with paper towels. Using light abdominal pressure, semen was collected in 1 ml syringes (0.02 ml scale) and immediately stored in ice (without direct contact with the syringes) at 4 ± 2°C (air temperature) protected from light (Magnotti, Castro, et al, 2018;Magnotti, Figueroa, et al, 2018). Samples contaminated with faeces or urine were discarded (Cosson et al, 2008).…”
Section: Reproductive Parametersmentioning
confidence: 99%
“…Sperm characteristics vary among fish species and are also influenced by season, reproductive period, environment, climate and the method of cell collection (Solis-Murgas, Felizardo, Ferreira, Andrade, & Veras, 2011). Some semen characteristics of mullet were described by Magnotti, Figueroa, et al (2018) and Magnotti, Castro, et al (2018), analysing cultured (F1)-also in first maturation, and wild species during reproductive migration. The cultured mullets presented better results for cell membrane integrity, motility rate and spermatic density (96 ± 13%, 83.6 ± 15.7% and 31.8 ± 2.9 × 10 9 ml −1 respectively).…”
The feed provided to breeding fish is one of the main factors influencing the quality of fish gametes. This study was carried out to evaluate the influence of ascorbic acid on growth, haematological parameters and sperm quality of Lebranche mullet males (Mugil liza). Six diets with different levels of ascorbic acid (0; 53; 107; 216; 482 and 708 mg/kg) were tested in triplicate for 75 days. During spermiation (first gonadal maturation), 144 individuals (205.7 ± 11.5 g and 25.7 ± 0.4 cm) were randomly distributed in 18 experimental tanks. Growth parameters were evaluated at the beginning and end of the experiment. Fish blood was collected to analyse glucose, total protein and erythrocyte count (EC) (n = 9). Fish (n = 12) from each treatment were euthanized to determine hepatosomatic index (HSI) and gonadosomatic index (GSI). Semen was collected to evaluate spermatic density, cell membrane integrity and sperm motility. No difference (p > .05) was found on growth parameters, GSI, HSI and total protein. However, EC was lower in fish fed without ascorbic acid (the control group). Ascorbic acid supplementation provided positive effects on sperm characteristics. Fish from treatments with 53 and 107 mg/kg presented the best results for motility time (133.30 ± 4.25 and 135.00 ± 2.77 respectively). Treatments with 107, 216 and 708 mg/kg provided the best results for motility rate (92.0 ± 2.9%, 93.0 ± 5.8% and 93.0 ± 5.8% respectively). Supplementation with 107 and 216 mg/kg provided the best results for plasma membrane integrity (70.12 ± 9.10% and 76.3 ± 3.1% respectively). Lower spermatic density was observed in fish without ascorbic acid supplementation, although no difference was found in sperm density among the treatments with ascorbic acid (p < .05). Considering these results, supplementation of dietary ascorbic acid between 107 and 216 mg/kg optimizes the spermatic quality in males of lebranche mullet.
“…The reproductive period began when females presented average oocyte diameter greater than 600 µm, value considered suitable for hormonal induction (Cerqueira et al, 2017). Males were mature and able to reproduce when they released semen after abdominal massage (Magnotti et al, 2018a(Magnotti et al, , 2018bCastro et al, 2019).…”
Section: Selection Of Fish For Hormonal Inductionmentioning
This study aimed to evaluate the reproductive response of mullets born in captivity (F1) using hormonal induction. Therefore, it was described maturation and induced spawning of mullet (Mugil liza) breeders born in captivity (F1). F1 males presented viable sperm at 11 months of age, with length of 25.7 ± 0.4 cm and weight of 205.7 ± 11.5 g. In contrast, at three years of age F1 females could be reproduced, with length of 47.4 ± 1.4 cm and weight of 1263.0 ± 64.6 g. Ten spawnings were conducted, and the average diameter of the oocytes at the time of hormonal induction, fertilization rate, total and relative fertility and hatching rate were recorded. Spawning was attained through hormonal induction in females with oocyte diameter greater than 600 µm and in males that released semen with abdominal massage. During this reproductive period, induced females produced a second group of mature oocytes so another hormonal induction was performed. The present study describes the induction of mullet breeders born in captivity and the possibility of females to be induced more than once during the same reproductive period.
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