&lt;b&gt;ONTOGENY OF POLYPEPTIDE YY (PYY) CELLS IN THE GUT OF CHICKEN: AN IMMUNOCYTOCHEMICAL &lt;/b&gt;&lt;b&gt;STUDY &lt;/b&gt;

Wilander, Erik; Abu-Sinna, G.; Shabaka, Hamza A. El; Lundberg, Jan M.; Tatemoto, Kazuhiko

doi:10.2220/biomedres.3.680

Cited by 8 publications

(4 citation statements)

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“…PYYimmunoreactive determinants not stained by this antiserum may nevertheless be present in tissue fixed in parabenzoquinone vapour and reactive with APP antiserum. By means of the same APP antiserum, Alumets et al (1978) and the present study have demonstrated immunoreactive cells in the proventriculus and small intestine much earlier than the PYY-immunoreactive cells were detected by E1-Salhy et al (1982b). If there are indeed no PYY cells in the proventriculus and ileum before hatching, then the cells stained there with the anti-APP serum could be PP rather than PYY cells.…”

Section: Discussionsupporting

confidence: 55%

“…Using an antiserum specific for PYY on chick embryonic tissue fixed in Bouin's fluid, E1-Salhy et al (1982b) demonstrated PYY-immunoreactive cells in the duodenum only; this was at hatching and at 18 days of incubation but no earlier. Unfortunately E1-Salhy's PYY antiserum has failed to demonstrate immunoreactive cells in parabenzoquinone vapour-fixed tissue in any region of the gastrointestinal tract of chicks at hatching (Rawdon, personal communication).…”

Section: Discussionmentioning

confidence: 97%

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The ontogeny and distribution of glucagon- and pancreatic polypeptide-immunoreactive cells in the gastrointestinal tract of the chicken

1990

Anat Embryol

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The times of first appearance and the distribution of APP- and glucagon-immunoreactive cells have been established in the embryonic chick gut between 11 days of incubation and hatching. These immunoreactive cell types appeared for the first time at 13 days of incubation, APP-immunoreactive cells in the duodenum and upper ileum and glucagon-immunoreactive cells in the proventriculus and duodenum. At 14 days, APP-immunoreactive cells were detected in the proventriculus and lower ileum and glucagon-immunoreactive cells in the pyloric region, upper and lower ileum. Thereafter both APP- and glucagon-immunoreactive cells increased in frequency until the numbers at hatching were approximated, APP-immunoreactive cells at 19 days and glucagon immunoreactive cells at 17 1/2 days of incubation. No APP- or glucagon-immunoreactive cells were found in the gizzard, caecum or rectum at any of the selected stages examined. When these types of endocrine cells first appeared, the surface epithelium of the gastrointestinal tract was relatively undifferentiated. A few glands were present in the proventriculus only, at this stage. Thereafter immunoreactive cells of both types were found in the glandular epithelium of the proventriculus, pyloric region and small intestine soon after morphogenesis had begun.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 97%

The ontogeny and distribution of glucagon- and pancreatic polypeptide-immunoreactive cells in the gastrointestinal tract of the chicken

1990

Anat Embryol

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“…Besides confirming these findings, we also observed the presence of PYY-immunoreactive cells and described their first appearance on day 12 of incubation. Thus, the reason why most previous studies (Alumets et al 1978;E1-Salhy et al 1982) failed to detect endocrine cells in the chicken gizzard could be that they examined only the true muscular gizzard, not also its diverticula. On the whole, our present data on the ontogeny of antral endocrine cells confirm almost all previous reports (Rawdon andAndrew 1981, 1993;E1-Salhy et al 1982;Andrew 1984;Alison 1989;Alison 1990).…”

Section: Discussionmentioning

confidence: 99%

“…Thus, the reason why most previous studies (Alumets et al 1978;E1-Salhy et al 1982) failed to detect endocrine cells in the chicken gizzard could be that they examined only the true muscular gizzard, not also its diverticula. On the whole, our present data on the ontogeny of antral endocrine cells confirm almost all previous reports (Rawdon andAndrew 1981, 1993;E1-Salhy et al 1982;Andrew 1984;Alison 1989;Alison 1990). Previous studies in various animal species have indicated that the antral gastrin/CCK-immunoreactive cells also costore other peptides (Rawdon and Andrew 1981;Flucher et al 1988;Burrell et al 1992;Sundler et al 1993).…”

Section: Discussionmentioning

confidence: 99%

Ontogeny, distribution and amine/peptide colocalization of chromogranin A- and B-immunoreactive cells in the chicken gizzard and antrum

1995

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The ontogeny and the distribution of chromogranin A (CgA)- and chromogranin B (CgB)-immunoreactive endocrine cells was studied in the chicken gizzard and gizzard-duodenal junction (also called pylorus or antrum) during embryonic and postnatal life. The same tissue sections were then double-immunostained to identify the CgA-and CgB-immunoreactive cells, with a panel of polyclonal antibodies raised against main gut amine/peptides. In the gizzard, positive cells were observed only in its two diverticula (proximal and distal caeca), where the first CgA- and CgB-immunoreactive cells were found on day 12 of incubation. They always remained moderate in number and co-stored mainly serotonin, gastrin/CCK and neurotensin. A few also co-stored somatostatin, but only during the embryonic period. Others co-stored PYY, but only after hatching. Co-localization with motilin was rare and never occurred with bombesin. In the chicken antrum, the first CgA- and CgB-immunoreactive cells were observed on day 12 of incubation and soon reached very high numbers. Antral positive cells showed almost the same co-localization pattern as the gizzard diverticula. Despite their high chromogranin content, the antral cells had weak argyrophilia, whereas in the gizzard diverticula the two staining patterns corresponded.

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Gastrointestinal hormones in birds: Morphological, chemical, and developmental aspects

1984

J. Exp. Zool.

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Historically, the enterochromaffin cell was the first endocrine cell type detected in avian gut; subsequently, a number of types of such cells were distinguished on the basis of the ultrastructural features of the secretory granules. More recently, immunocytochemical procedures have revealed somatostatin-, pancreatic polypeptide (PP)-, polypeptide YY-, glucagon-, secretin-, vasoactive intestinal peptide (VIP)-, gastrin-, cholecystokinin-, neurotensin-, bombesin-, substance P-, enkephalin-, motilin-, and FMRFamide-like immunoreactivity in avian gastrointestinal endocrine cells. Most endocrine cells are located in the antrum; there are a number in the proventriculus and small intestine but few in the gizzard, cecum, and rectum. Several avian gastroenteropancreatic hormones, including glucagon, VIP, secretin, bombesin, neurotensin, and PP, have been isolated and sequenced. They resemble the equivalent mammalian peptides in terms of molecular size but differ in amino acid composition and sequence; some (e.g., VIP) differ only in minor respects, others (e.g., secretin) more radically. Gastrointestinal endocrine cells appear late in development; available data indicate that few types are recognized by either immunocytochemistry or electron microscopy before 16 days of incubation. Experimental evidence has shown that at least the majority of gut endocrine cells are of endodermal origin and are not derived from the neural crest or neuroectoderm as earlier proposed. In early embryos, the progenitors of gastrointestinal endocrine cells are more widespread than are the differentiated cells in chicks at hatching. This, along with other observations, raises the question of factors that might influence the differentiation of gut endocrine cells.

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<b>ONTOGENY OF POLYPEPTIDE YY (PYY) CELLS IN THE GUT OF CHICKEN: AN IMMUNOCYTOCHEMICAL </b><b>STUDY </b>

Cited by 8 publications

References 1 publication

The ontogeny and distribution of glucagon- and pancreatic polypeptide-immunoreactive cells in the gastrointestinal tract of the chicken

The ontogeny and distribution of glucagon- and pancreatic polypeptide-immunoreactive cells in the gastrointestinal tract of the chicken

Ontogeny, distribution and amine/peptide colocalization of chromogranin A- and B-immunoreactive cells in the chicken gizzard and antrum

Gastrointestinal hormones in birds: Morphological, chemical, and developmental aspects

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