&lt;b&gt;Mapping and validation of molecular markers of genes &lt;i&gt;Dt1&lt;/i&gt; and &lt;i&gt;Dt2&lt;/i&gt; to determine the type of stem growth in soybean

Vicente, Dorival; Schuster, Ivan; Lazzari, Fabiane; Paranzini, Jonas Pedro Dolfini; Oliveira, Marco Antônio Rott de; Prete, Cássio Egídio Cavenaghi

doi:10.4025/actasciagron.v38i1.26216

Cited by 8 publications

(6 citation statements)

References 12 publications

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“…The dominance of the IDT stem has also been reported earlier in chickpea [5,17]. The dominant nature of the IDT stem growth was also noticed in Glycine max [1], Vicia faba [3] and Cajanus cajan [7,20]. The F 2 plants derived from BG 362 (IDT) x BG 3078-1 (SDT) cross segregated into 138 IDT: 45 SDT (Table 1).…”

Section: Discussionsupporting

confidence: 72%

Unraveling Genetics of Semi-Determinancy and Identification of Markers for Indeterminate Stem Growth Habit in Chickpea (Cicer Arietinum L.)

Nagraj

Hegde

Nimmy

et al. 2021

Preprint

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BackgroundChickpea (Cicer arietinum L.) is predominantly an indeterminate plant and tends to generate vegetative growth when the ambient is conducive for soil moisture, temperature and certain other environmental conditions. The semi-determinate (SDT) types are comparatively early, resistant to lodging and found to be similar in their yield potential to indeterminate (IDT) lines. Indeterminate types and semi-determinate genotypes are found to be similar during early stage, which makes it difficult to distinguish between them. Thus, there is a need to identify molecular marker linked either to indeterminate or semi-determinate plant type. The present study was carried out to study the genetics of semi-determinacy and identify molecular markers linked to stem growth habit. MethodsThe study was undertaken in the cross involving BG 362(IDT) X BG 3078-1(SDT). All F1 plants were indeterminate, which indicates that indeterminate stem type is dominant over semi-determinate. In further advancement to F2 generation, F2 plants are segregated in the ratio of 3(Indeterminate): 1(Semi-determinate) that indicates that the IDT and SDT parents which are involved in the cross differed for a single gene. The segregation pattern observed in F2 is confirmed in F3 generation. The parental polymorphic survey was undertaken for molecular analysis using total of 246 SSR markers, out of which 41 polymorphic markers were found to distinguish the parents and were utilized for bulked segregant analysis (BSA). ResultsThe segregation pattern in F2 indicates that the IDT(Indeterminate) and SDT(Semi-determinate) parents which are involved in the cross differed for single gene. The segregation pattern of F2 and F3 derived from the cross BG 362 (IDT) x BG 3078-1 (SDT) confirmed the genotypic structure of the newly found SDT genotype BG 3078-1 as dt1dt1Dt2Dt2. Three SSR markers TA42, Ca_GPSSR00560 and H3DO5 were found to be putatively linked to Dt1 locus regulating IDT stem growth habit.ConclusionOur results indicate that the SSR markers identified for Dt1 locus helps to differentiate stem growth habit of chickpea in its early growth stage itself and can be efficiently utilized in Marker Assisted Selection (MAS) for changed plant type in chickpea.

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Section: Discussionsupporting

confidence: 72%

Unraveling Genetics of Semi-Determinancy and Identification of Markers for Indeterminate Stem Growth Habit in Chickpea (Cicer Arietinum L.)

Nagraj

Hegde

Nimmy

et al. 2021

Preprint

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“…Kaur and Banga [ 20 ] mapped Indian mustard determinate growth habit gene ( Sdt1 ), which was flanked by 2 SSR markers at distances of 15.9 cM and 14.0 cM. Vicente et al [ 23 ] mapped the molecular markers of genes Dt1 and Dt2 in soybean, and sat_064 marker associated with Dt2 exhibited a recombination frequency of 19.4%. Nevertheless, most of these QTL/gene from above species were primarily located in a large genome scale, since the QTL regions were not small enough, it was far from candidate gene predication or map-based gene cloning.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, extensive genetic and molecular studies have been implemented in these plants. Genes for determinacy of common bean, cucumber, soybean, maize and Brassica juncea have been located by QTL mapping [ 17 – 22 ], molecular makers associated with determinacy gene of faba bean, pigeonpea, common bean and soybean have been also revealed by linkage analysis or whole-genome scanning based association mapping [ 12 , 13 , 15 , 23 , 24 ]. Besides, candidate gene or homologous sequences analysis or ortholog gene sequencing approach has also been employed on common bean, soybean and pigeonpea determinacy gene isolation and characterization [ 25 – 27 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic dissection and fine mapping of a novel dt gene associated with determinate growth habit in sesame

et al. 2018

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BackgroundAs an important oil crop, growth habit of sesame (Sesamum indicum L.) is naturally indeterminate, which brings about asynchronous maturity of capsules and causes loss of yield.ResultsThe genetic basis of determinate growth habit in sesame was investigated by classical genetic analysis through multiple populations, results revealed that it was controlled by an unique recessive gene. The genotyping by sequencing (GBS) approach was employed for high-throughput SNP identification and genotyping in the F2 population, then a high density bin map was constructed, the map was 1086.403 cM in length, which consisted of 1184 bins (13,679 SNPs), with an average of 0.918 cM between adjacent bins. Based on bin mapping in conjunction with SSR markers analysis in targeted region, the novel sesame determinacy gene was mapped on LG09 in a genome region of 41 kb.ConclusionsThis study dissected genetic basis of determinate growth habit in sesame, constructed a new high-density bin map and mapped a novel determinacy gene. Results of this study demonstrate that we employed an optimized approach to get fine-accuracy, high-resolution and high-efficiency mapping result in sesame. The findings provided important foundation for sesame determinacy gene cloning and were expected to be applied in breeding for cultivars suited to mechanized production.

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“…This is the first report on the identification of markers linked to Dt1 locus in chickpea. However, a closely linked marker TA34 for stem growth habit has been identified in soybean (Vicente et al 2016) and IDT growth habit locus (Dt1) was mapped to CcLG03 (Varshney et al 2013;Saxena et al 2017) in pigeonpea.…”

Section: Plant Morphology Of Sdt Chickpea Genotype Bg 3078-1mentioning

confidence: 99%

Genetics of semi-determinacy and identification of molecular marker linked to Dt1 locus in chickpea (Cicer arietinum L.)

Harshavardhana

Hegde

Tripathi

et al. 2019

IJGPB

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Chickpea is predominantly an indeterminate (IDT) plant due to which high fertility and irrigation have adverse effect on seed yield. The semi-determinate (SDT) types are relatively early, lodging resistant and found to be similar in their yield potential to that of IDT genotypes. However, the inheritance of SDT stem type is not well understood in chickpea. The present investigation was aimed at studying the genetics of SDT stem type and identifying molecular marker linked to Dt1 locus through Bulked Segregant Analysis (BSA). The genetics of semi-determinacy was studied in BGD 72 (IDT) x BG 3078-1(SDT) cross in which the genetic constitution of BGD 72 was already known as Dt1Dt1Dt2Dt2 based on the previous study. The F1 was IDT indicating the dominance of indeterminacy over semideterminacy. The segregation in F2 and F3 revealed that SDT stem growth in the new-found genotype BG3078-1 was governed by a single dominant gene Dt2 and its genotype designated as dt1dt1Dt2Dt2. The study of polymorphic survey between BGD-72(IDT) and BG 3078- 1(SDT) using 581 SSR markers found 50 markers polymorphic. The BSA using 50 polymorphic markers has identified TA42 and TR29 as polymorphic between IDT and SDT parents as well as IDT and SDT bulks and hence considered putatively linked to Dt1 locus governing IDT stem growth in chickpea. The two linked markers were validated in 15 IDT and 15 SDT F2 plants individually. These two markers were also validated in 3 IDT, 8SDT and 3 DT genotypes. This is the first report on the identification of a molecular marker associated with stem growth habit in chickpea.

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<b>Mapping and validation of molecular markers of genes <i>Dt1</i> and <i>Dt2</i> to determine the type of stem growth in soybean

Cited by 8 publications

References 12 publications

Unraveling Genetics of Semi-Determinancy and Identification of Markers for Indeterminate Stem Growth Habit in Chickpea (Cicer Arietinum L.)

Unraveling Genetics of Semi-Determinancy and Identification of Markers for Indeterminate Stem Growth Habit in Chickpea (Cicer Arietinum L.)

Genetic dissection and fine mapping of a novel dt gene associated with determinate growth habit in sesame

Genetics of semi-determinacy and identification of molecular marker linked to Dt1 locus in chickpea (Cicer arietinum L.)

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