<b><i>Lactobacillus reuteri</i></b> Influences Regrowth of Mutans Streptococci after Full-Mouth Disinfection: A Double-Blind, Randomised Controlled Trial

Abstract

This study assessed whether the persistence of Lactobacillus reuteri DSM 17938 and ATCC PTA 5289 in saliva could delay the regrowth of mutans streptococci (MS) after a full-mouth disinfection with chlorhexidine (CHX). A randomised, double-blind, placebo-controlled study with a 6-week intervention period and 3- and 6-month follow-up was performed. 62 healthy subjects with moderate to high counts of MS were randomly assigned to a test group (n = 32) or a placebo group (n = 30). Before onset of the intervention, subjects received two sessions of professional cleaning, flossing, and application of CHX varnish and rinsed their mouth with a CHX solution between the sessions (2 days). Thereafter, the test group used probiotic lozenges (2/day) containing L. reuteri (DSM 17938 and ATCC PTA 5289; 1 × 10⁸ CFU of each strain), and the placebo group used identical lozenges lacking the lactobacilli. Saliva samples were collected and cultured onto selective media, and isolates of L. reuteri as well as DNA directly extracted from saliva were tested by polymerase chain reaction (PCR) with specific primers. Presence of salivary MS was analysed with a chair-side test. L. reuteri was frequently detected by culture during the intervention period but in only 3 test group subjects at follow-ups. Regrowth of MS statistically significantly differed depending on the presence or absence of L. reuteri DSM 17938 detected by PCR. We conclude that cultivable L. reuteri strains may only sporadically be confirmed after termination of the intervention, but subjects with PCR-detected L. reuteri demonstrated slower regrowth of MS.

Oral Microbiota Shift after 12-Week Supplementation with Lactobacillus reuteri DSM 17938 and PTA 5289; A Randomized Control Trial

“…reuteri DSM 17938 and PTA 5289 strains, which is similar to findings from other studies with these strains or L . rhamnosus GG [ 33 , 34 , 64 , 65 ], indicates that the expected effect is individual. Installation of probiotic lactobacilli likely reflects genetic variations in host receptor epitopes for bacterial binding, such as salivary gp340, MUC5, MUC7, and ABO antigen epitopes [ 10 , 66 – 68 ].…”

“…Aliquots of saliva were plated onto Rogosa agar (Merck, Darmstadt, Germany) to obtain Lactobacillus counts and on selective agar for tentative identification of the L . reuteri (DSM 17938 and PTA 5289) strains [ 34 ]. All plates were anaerobically incubated at 37°C for 48–72 h, except L .…”

“…L . reuteri DSM 17938 and PTA 5289-specific PCR were identified using KAPA2G Robust HotStart PCR Ready Mix (2×) (Kapa Biosystems, Boston, MA, USA) and strain-specific primers [ 34 ]. Briefly, 2 μL of DNA extract was added to a total reaction volume of 25 μL (containing 12.5 μL of master mix and each primer pair at a concentration of 0.5 μM).…”

Effects of Lactobacillus reuteri supplementation on the gut microbiota in extremely preterm infants in a randomized placebo-controlled trial

“…The single-copy gene Lactobacillus reuteri unknown extracellular protein lr1694 (GenBank accession number: DQ074924.1) is specific for the supplemented probiotic L. reuteri strain used in this study 52 . The lr1694 gene was amplified in 20 mL qPCR reactions consisting of 2 mL of 10-fold diluted DNA, 1X SsoFast TM EvaGreenâ Supermix (Bio-Rad, Hercules, CA), 300 mM of forward primer…”

Der prophylaktische Einsatz von Probiotika

“…Sollte letzteres der Fall sein, könnten Profile des oralen Mikrobioms zukünftig als diagnostische Biomarker dienen [21]. [25] probiotisches Milchpulver [26] Getreidebrei [27] xylithaltige Lutschtabletten [46,47] L. rhamnosus L. reuteri probiotischer Joghurt [28] probiotisches Milchpulver [29] probiotische, fluorhaltige Milch [30] probiotische Lutschtabletten [43][44][45] L. reuteri L. brevis probiotische Lutschtabletten [31] Drops [32] probiotische Lutschbonbons [48] L. brevis probiotische Lutschbonbons [33] Probiotika und Parodontitis Im Zusammenspiel mit wirtsspezifischen Faktoren entfalten bestimmte Bakterien auch parodontalpathogene Eigenschaften. In den letzten Jahren etablierte sich für die parodontalpathogenen Schlüsselspezies die Bezeichnung "red complex": Porphyromonas gingivalis, Tannerella forsythia und Treponema denticola [37].…”