<b>Larvicidal, cytotoxic and genotoxic effects of aqueous leaf extract of <i>Jatropha mollissima</i> (Pohl) Baill

Abstract

ABSTRACT. The present study aimed to evaluate the larvicidal effect of aqueous leaf extract from Jatropha mollissima on the larvae of Aedes aegypti and analyze its cytotoxic and genotoxic activity in the Allium cepa test. Larvae of the mosquito were exposed to the negative and positive controls (distilled water and diflubenzuron, 0.003 mg mL -1 , respectively) and to leaf extract concentrations of 0.001, 0.005, 0.01, 0.02, 0.04, 0.06, 0.08 and 0.1 mg mL -1 . The mortality rate was evaluated every 24 hours over five days. For the cytotoxic and genotoxic analyses, roots of A. cepa were exposed to the negative (distilled water) and positive control (trifluralin, 0.84 ppm) and to different leaf extract concentrations (0.01, 0.1, 1 and 10 mg mL Efeitos larvicida, citotóxico e genotóxico do extrato aquoso das folhas de Jatropha mollissima (Pohl) Baill RESUMO. O presente estudo teve como objetivo avaliar o efeito larvicida do extrato aquoso das folhas de Jatropha mollissima sobre as larvas de Aedes aegypti e analisar sua atividade citotóxica e genotóxica no teste Allium cepa. As larvas do mosquito foram expostas aos controles negativo e positivo (água destilada e diflubenzuron, 0,003 mg mL -1 , respectivamente) e ao extrato foliar nas concentrações de 0,001; 0,005; 0,01; 0,02; 0,04; 0,06; 0,08 e 0,1 mg mL -1 . A taxa de mortalidade foi avaliada a cada 24horas durante cinco dias. Para as análises citotóxica e genotóxica, as raízes de A. cepa foram expostas ao controle negativo (água destilada) e positivo (trifluralina, 0,84 ppm) e nas concentrações (0,01; 0,1; 1 e 10 mg mL -1 ) do extrato foliar por 24 horas. Análises estatísticas foram realizadas pelo teste de Kruskal-Wallis (p < 0,05). O extrato foliar apresentou atividade larvicida promissora nas concentrações de 0,08 e 0,1 mg mL -1 , e nenhuma das concentrações avaliadas em A. cepa exibiu efeito citotóxico ou genotóxico. Uma vez demonstrada a ação larvicida de J. mollissima e a ausência de toxicidade celular, mais estudos são recomendados para determinar o mecanismo de ação do extrato como um possível larvicida natural.

In vivo assessment of reversing Cisplatin-Induced nephrotoxicity using Jatropha mollissima crude extract and its potential cytotoxicity

“…The anatomical, physiological and histopathological results reveal no significant changes among any of the treated groups. Similar finding have been gathered in previous studies, by using the aquas extract of J. mollissima ( Araújo et al, 2018 , de Queiroz Neto et al, 2019 ). Owing to the high content of many phytochemicals such as flavonoids, phenols, tannins and saponins, the extract exhibited strong antioxidant activity, which could be used as anticancer agent or in wound healing applications ( Abdul Khalil et al, 2020 , Goswami and Kuril, 2019 , Yahya et al, 2020a , Yahya et al, 2020b ).…”

“…Many of Jatropha species including J. mollissima are known for their antibacterial activity ( Braquehais et al, 2016 , da Rocha and Dantas, 2009 ), while the aqueous extract of the leaves was found to be co-adjuvant in snakebites treatment ( Gomes et al, 2016 ). Stem aqueous extract has been investigated against the nematode Haemonchus contortus in sheep ( Ribeiro et al, 2014 ), and the larvicidal activity against Aedes aegypti ( Araújo et al, 2018 ). Milkylooking sap (latex) that produced during the injury of J. mollissima have been used by the ancients for wound healing, controlling hemorrhages, anti-infections, and to minimize the inflammations ( de Queiroz Neto et al, 2019 ).…”