<b>INSOLUBILITY OF PROTEINS IN ALZHEIMER’S </b><b>BRAIN </b>

Abstract

Accumulating-evidence indicates that the aberrant deposition of amyloid [3 protein is the main pathological feature of Alzheimer's disease. However, the recent studies on apolipoprotein E .94 gene polymorphism as a risk factor of the disease may indicate multifactorial nature of the etiology of Alzheimer's disease. In this context, it will be worth to reanalyse proteins of Alzheimer's brains to explore other proteins related to the disease. In the present work we found an increase in the SDS/fiME-insoluble proteins in Alzheimer's brain which could be solubilized for SDS-PAGE after further treatment with chloroformmethanol (CM). We suggest that the CM treatment disrupts hydrophobic interactions between proteins and lipids in the insoluble complexes and make the proteins soluble. Proteins soluble in CM showed some differences in SDS-PAGE patterns between the Alzheimer's and the control brains.