<b>Etiological and molecular profile of pathogens causing clinical mastitis, and antimicrobial use in dairy herds</b>

Abstract

Pirassununga 2017Autorizo a reprodução parcial ou total desta obra, para fins acadêmicos, desde que citada a fonte. DADOS INTERNACIONAIS DE CATALOGAÇÃO NA PUBLICAÇÃO(Biblioteca Virginie Buff D'Ápice da Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo) T. 3560 Tomazi, Tiago FMVZ Etiological and molecular profile of pathogens causing clinical mastitis, and antimicrobial use in dairy herds. / Tiago Tomazi. --2017. 187 f. : il.Título traduzido: Perfil etiológico e molecular de patógenos causadores de mastite clínica, e uso de antimicrobianos em rebanhos leiteiros. AGRADECIMENTOS (ACKNOWLEDGEMENTS)Primeiramente agradeço a Deus pelas oportunidades que me tens dado de testemunhar o amor que tens por mim. Agradeço também ao Senhor pelos animais, seres divinos e frutos do meu trabalho. É em suas mãos que entrego as rédeas da minha vida.Agradeço também à Nossa Senhora de Lourdes por iluminar o meu caminho e a minha família. E por sempre atender aos meus pedidos.A minha família por jamais deixar faltar confiança, apoio, carinho, compreensão e amor.E por provar que um coração pode se sentir aquecido e amado, mesmo a quilômetros de distância. Muito obrigado a minha mãe, pai e irmãos Lucas e Lutiene.Aos meus avós Alcides, Elvira, Alberto e Antonieta por me ensinar o "respeito", uma das virtudes mais belas de um ser humano.A minha namorada, noiva e confidente Ana Carolina de Campos Henrique, que tem um coração enormemente capaz de suportar meu amor e todos os outros sentimentos às vezes não tão prazerosos. Meus momentos felizes ficam ainda mais felizes ao seu lado; e meus momentos de tristeza e fraqueza logo se desfazem na presença de sua palavra e companhia. quarter-cases of CM were recorded and the most frequently isolated pathogens were Escherichia coli (6.6% of total cultures), Streptococcus uberis (6.1%), and Streptococcus agalactiae (5.9%). The majority of CM cases were mild (60.3%), while 34.1% were moderate and 5.6% severe. Overall, the IRCM was 9.7 quarter-cases per 10,000 quarter-days at risk (QDAR), and the only herd-level parameter associated with overall IRCM was BMSCC, in which the highest IRCM was observed for herds with BMSCC >600.000 × 10 3 cells/mL. In the models evaluating the specific-pathogen groups, IRCM with isolation of major contagious pathogens was associated with BMSCC, milk yield and housing system. For the evaluation of other Gram-positive pathogens, the IRCM was higher in the rainy season of 2015 in comparison with the other seasonal categories. In addition, for the model evaluating the Gram-negative group, the IRCM was highest in herds with BMTBC >30 × 10 3 cfu/mL. The Study 2 aimed to characterize the treatment profile and quantify the antimicrobial consumption for treatment of CM in dairy herds; and to determine the association of antimicrobial use (AMU) and the same herd-level descriptors as described in the Study 1. Data on treatment practices and AMU were obtained from 19 dairy herds for a period of 12 months per herd. The AMU for treatment of CM was quantifie...

Antimicrobial susceptibility patterns of Escherichia coli phylogenetic groups isolated from bovine clinical mastitis

“…Isolates were selected from a collection of E. coli previously identified by microbiological culture during the data collection of a study on epidemiological characterization of CM in 20 dairy herds of southeastern Brazil (Tomazi, 2017). Herds were conveniently selected based on a client list of the Qualileite Lab (Mastitis and Milk Quality Research Laboratory at University of Sao Paulo, Brazil).…”

“…During the study period (March 2014 to January 2016), 276 E. coli isolates were identified by microbiological culture of 4,212 individual quarter milk samples collected from 2,637 cows with CM (Tomazi, 2017). Briefly, a 0.01-mL sterile loop was used to plate milk samples onto trypticase soy agar plates (BBL-Becton Dickinson and Co., LePoint de Claix, France) enriched with 5% bovine blood, and plates were incubated aerobically at 37°C.…”

“…Descriptive data of herds from which E. coli isolates were identified are presented in Table 2. Escherichia coli isolates were identified from cases of CM in 19 of 20 dairy herds evaluated in an epidemiological study on the characterization of CM (Tomazi, 2017). One herd (named herd N) had no isolation of E. coli during the study period.…”