<b>Cryopreservation of semen of Thailand tilapia (<i>Oreochromis</i> spp.) fed diet with different oil sources

Abstract

ABSTRACT. This study evaluated the quality of sperm cryopreserved with dimethylsulfoxide (DMSO) or methanol, of Thailand tilapia (Oreochromis spp.) fed diet with different oil sources. eighty Thailand tilapia fed different oil sources (T1: linseed oil, T2: soybean oil, T3: fish oil, T4: corn oil) and were analyzed as for motility rate, and duration of motility in 'fresh sperm' and after cryopreservation. No significant difference was found for the final weight, total length, rate and duration of motility of fresh sperm in the different treatments. Significant differences were observed between fish, corn and linseed oil treatments on the rate of motility relative to other different oil sources with the cryoprotectant DMSO prior to freezing. After thawing, fish fed diet supplemented with fish oil presented a significantly higher rate and duration of sperm motility. Nevertheless, for better reproductive parameters after freezing, it is recommended the use of fish oil associated with cryoprotectant methanol.Keywords: freeze, cryoprotectant, DMSO, methanol.Criopreservação de sêmen de tilápia do Nilo, alimentada com diferentes fontes de óleo RESUMO. Este estudo avaliou a qualidade do esperma criopreservados com dimetilsulfóxido (DMSO) ou metanol, da tilápia Tailandesa (Oreochromis spp.), alimentada com diferentes fontes de óleo. Foram utilizadas 80 tilápias Tailandesas alimentadas com quatro fontes de óleo (T1: óleo de linhaça, T2: óleo de soja, T3: óleo de peixe, T4: óleo de milho). Foram analisadas a taxa de motilidade e a duração de motilidade do sêmen in natura após a criopreservação. Nenhuma diferença significativa foi encontrada para o peso final, comprimento total, taxa e duração da motilidade do esperma fresco nos diferentes tratamentos. Foram observadas diferenças significativas para óleo de milho, de peixe, de linhaça sobre a taxa de motilidade, utilizando o crioprotector DMSO antes da congelação. Após a descongelação, o peixe submetido a uma dieta suplementada com óleo de peixe apresentava uma taxa significativamente mais elevada e duração da motilidade dos espermatozoides. No entanto, para os melhores parâmetros de reprodução após a congelação, é recomendado o uso de óleo de peixe associado com crioprotetor metanol.

Efectos del triclosán sobre la movilidad espermática en Oreochromis spp.

“…On the other hand, it was evidenced that the control fish (0 µg/L) showed rapid motility at pH 7 and 9 between 73 and 95%. The results are consistent with the studies conducted by Navarro Rodrigo et al (22) who verified similar results in sperm motility studies reported in Oreochromis spp. between 89 and 100%; Guerrero -Quiroz et al (23) observed motility percentages higher than 94% in this species; Triastuti et al (24) reported motility percentages of 93.4% in tilapia.…”

Semen characteristics of the amazonian jundiá (Leiarius marmoratus Gill, 1870)

“…Other studies report 90% (KAVAMOTO et al, 1999) and 97% (MILIORINI et al, 2011) normal sperm in Prochilodus lineatus, 64% in Leporinus elongatus (STREIT Jr. et al 2008b), 57% in the dorado Salminus maxillosus (STREIT Jr. et al 2008a), 53% in the cachara Pseudoplatystoma reticulatum (STREIT Jr. et al 2012) and 16% in the Nile tilapia Oreochromis niloticus (MATAVELI et al 2007). The variation of normal semen percentage found among the species is influenced by several factors such as the nutritional status of the fish, size, age, and time of the year (VIVEIROS; GODINHO, 2009;SANCHES et al, 2011;NAVARRO et al, 2014).…”

Dietary supplementation ofTribulus terrestrisextract improves growth and reproductive performances of the male Nile tilapia (Oreochromis niloticus)

“…Semen was diluted 1:4 with 0.9% Nacl for microscopic counting using haemocytometer. At the same time, the following parameters were estimated; [(motility that divide into rapid progressive, sluggish progressive, nonprogressive and immotile), (morphology that divided into normal sperm and abnormal sperm), vitality by haematoxylin and eosin stain was done (Navarro, Navarro, Felizardo, Murgas, & Pereira, 2014).…”