&lt;b&gt;Clinical studies on changes in purine compounds in blood and urine by the simultaneous administration of febuxostat and inosine, or by single administration of each&lt;/b&gt;

Kamatani, Naoyuki; Hashimoto, Masahiro; Sakurai, Kuniko; Gokita, Kaoru; Yoshihara, Junko; Sekine, Minoru; Mochii, Masa-aki; Fukuuchi, Tomoko; Yamaoka, Noriko; Kaneko, Kiyoko

doi:10.6032/gnam.41.171

Cited by 9 publications

(13 citation statements)

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“…This is because, unlike human blood, XDH / XO is abundant in such organs as liver and kidney 18) . When inosine alone was orally administered to humans, hypoxanthine did not increase because it was degraded by XDH / XO 12) . In the present study, hypoxanthine Thus, our present study suggests that the combined use of inosine and a XDH / XO inhibitor may be useful for the patients with hemolytic anemia caused by glycolytic enzyme deficiencies, and other abnormalities including thalassemia, and hemoglobinopathy.…”

Section: Resultsmentioning

confidence: 97%

“…We reported a method to enhance ATP in vivo in humans by clinical studies on healthy subjects 12) . The measurement of ATP was performed in peripheral blood.…”

Section: N T H a L A S S E M I A A N D H E M O G L O B I N O P A T mentioning

confidence: 99%

“…Since ATP is present at high concentrations only within cells but not in the extracellular fluid, most of ATP in blood exists in erythrocytes. Therefore, it is suggested that the method reported in the previous paper 12) enhanced ATP in erythrocytes.…”

Section: N T H a L A S S E M I A A N D H E M O G L O B I N O P A T mentioning

confidence: 99%

“…In the present study, we attempted to examine whether ATP is enhanced in vitro when erythrocytes from a healthy subject and patients with thalassemia and hemoglobinopathy were incubated with inosine in saline. Metabolic pathway related to the present study is shown in Fig 1. Although a xanthine dehydrogenase (XDH)/ xanthine oxidase (XO) inhibitor febuxostat was added in addition to inosine in the previous clinical studies 12) , inosine alone was used in the present study. This is because XDH / XO activity is very low in human peripheral blood (Fig 1) .…”

Section: N T H a L A S S E M I A A N D H E M O G L O B I N O P A T mentioning

confidence: 99%

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<b>In vitro enhancement of ATP in human erythrocytes from a healthy subject and two patients with thalassemia and hemoglobinopathy.</b>

Kamatani

Furihata

Taniguchi

et al. 2018

GOUT AND NUCLEIC ACID METABOLISM

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Objective : To examine whether the supplementation of inosine augments ATP in vitro in human erythrocytes incubated in saline. Methods : Peripheral blood was drawn from each of three subjects, i.e. one healthy male and two males with thalassemia and hemoglobinopathy. After washing the erythrocytes in saline, they were suspended in saline to which inosine was added to final concentrations of 0, 0.5 and 2.5 mM. The suspension was incubated at 37 °C for 1 or 3 hours, and 0.5 ml ice cold 8% perchloric acid was added to the 0.5 ml erythrocytecontaining solution. After removing precipitates and perchloric acid, the supernatant was submitted to HPLC for the measurement of ATP. Results : Since the blood samples of the two subjects with thalassemia and hemoglobinopathy were transported from the clinics to the laboratory, ATP in the blood decreased considerably during the transportation. However, the reduction of ATP with time was observed in the erythrocytes in saline obtained from each of the three subjects during the incubation from 1 hour to 3 hours. In addition, dosedependent suppression of the decrease of ATP with inosine was observed in all the three cases at both 1 hour and 3 hour incubation times. Conclusions : Incubation of erythrocytes from a healthy subject and two thalassemia / hemoglobinopathy patients in saline at 37 °C resulted in timedependent decreases of ATP. Supplementation of inosine to the solutions resulted in the suppression of the decreases of ATP in a dosedependent manner.

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Section: Resultsmentioning

confidence: 97%

“…We reported a method to enhance ATP in vivo in humans by clinical studies on healthy subjects 12) . The measurement of ATP was performed in peripheral blood.…”

Section: N T H a L A S S E M I A A N D H E M O G L O B I N O P A T mentioning

confidence: 99%

Section: N T H a L A S S E M I A A N D H E M O G L O B I N O P A T mentioning

confidence: 99%

Section: N T H a L A S S E M I A A N D H E M O G L O B I N O P A T mentioning

confidence: 99%

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<b>In vitro enhancement of ATP in human erythrocytes from a healthy subject and two patients with thalassemia and hemoglobinopathy.</b>

Kamatani

Furihata

Taniguchi

et al. 2018

GOUT AND NUCLEIC ACID METABOLISM

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“…Одновременный прием фебуксостата и инозина (пуриновый нуклеозид) способствует повышению уровня как гипоксантина, так и АТФ в периферической крови. [45].…”

Section: повреждение легких индуцированное паракватомunclassified

Some opportunities of using xanthine oxidase inhibitors

Желябина¹,

Елисеев²

2019

Sovremennaâ revmatologiâ

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В обзоре представлены современные взгляды на роль фермента ксантиноксидазы (КСО) в патогенезе ряда заболеваний. Показано, что активность ксантиноксидазы (КСО) может быть связана с развитием и прогрессированием не только подагры, но, возможно, и сахарного диабета, артериальной гипертензии и другой сердечно-сосудистой патологии. Парадигма связи гиперурикемии (ГУ) с сердечно-сосудистыми заболеваниями и хроническими заболеваниями почек претерпела ряд изменений -от скептицизма до получения доказательств их серьезного негативного влияния. При этом установлено, что КСО является ключевым ферментом, участвующим в синтезе МК в качестве конечного метаболита адениннуклеотидов и широко представлена в разных органах (печень, кишечник, легкие, почки, сердце и мозг) в плазме крови. КСО играет значимую роль в синтезе не только МК, но и свободных радикалов супероксидов. Существует два возможных механизма, связывающих метаболизм и воспаление, в которых участвует МК: воспаление, активированное кристаллизацией МК, и генерация свободных радикалов супероксидов при ее синтезе. Роль активных форм кислорода обсуждается при ряде патологических состояний: индукция апоптоза/некроза, подавление экспрессии многих генов, активация клеточных сигнальных каскадов, окислительное повреждение ДНК и липидов, воспаление, метаболические расстройства, атеросклероз и др. Ингибирование этих механизмов приводит к снижению синтеза и отложения МК в костно-суставных и других тканях. Соответственно, КСО является подтвержденной мишенью для лечения подагры и, возможно, других состояний, связанных с ГУ. После появления в практике нового ингибитора КСО (иКСО), достижение целевого уровня МК стало реальным более чем у 80% пациентов с подагрой. Потому растет интерес к оценке возможностей иКСО при разных патологических состояниях. Ключевые слова: ксантиноксидаза; активные формы кислорода; ингибиторы ксантиноксидазы; ксантиндегидрогеназа; ксантиноксиредуктаза; гиперурикемия. Контакты: Ольга Владимировна Желябина; olga-sheliabina@mail.ru Для ссылки: Желябина ОВ, Елисеев МС. Некоторые потенциальные возможности применения ингибиторов ксантиноксидазы. Современная ревматология. 2019;13(1):114-120. Some opportunities of using xanthine oxidase inhibitorsZhelyabina O.V., Eliseev M.S. V.A. The review presents modern concepts of the role of xanthine oxidase (XO) in the pathogenesis of a number of diseases. It has been shown that XO activity can be associated with the development and progression not only of gout, but, possibly, diabetes mellitus, hypertension, and other cardiovascular diseases.The paradigm of an association of hyperuricemia (HU) with cardiovascular and chronic renal diseases has undergone certain changes -from skepticism to obtaining the evidence of its serious negative impact. Moreover, XO has been established to be a key enzyme involved in the synthesis of uric acid (UA) as a final adenine nucleotides metabolite, and is extensively present in various organs (the liver, intestine, lung, kidneys, heart, and brain) and in plasma. XO plays an important role in the ...

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Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia

et al. 2022

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<b>Clinical studies on changes in purine compounds in blood and urine by the simultaneous administration of febuxostat and inosine, or by single administration of each</b>

Abstract: Clinical studies on changes in purine compounds in blood and urine by the simultaneous administration of febuxostat and inosine, or by single administration of each 受付：2017年5月13日，受理：2017年7月13日 1

Cited by 9 publications

References 13 publications

<b>In vitro enhancement of ATP in human erythrocytes from a healthy subject and two patients with thalassemia and hemoglobinopathy.</b>

<b>In vitro enhancement of ATP in human erythrocytes from a healthy subject and two patients with thalassemia and hemoglobinopathy.</b>

Some opportunities of using xanthine oxidase inhibitors

Nociceptor-derived Reg3γ prevents endotoxic death by targeting kynurenine pathway in microglia

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