&lt;b&gt;Analysis of the genetic diversity of &lt;i&gt;Eucalyptus cladocalyx&lt;/i&gt; (sugar gum) using ISSR markers

Ballesta, Paulina; Mora, Freddy; Contreras‐Soto, Rodrigo Iván; Ruíz, Eduardo; Perret, Sandra

doi:10.4025/actasciagron.v37i2.19307

Cited by 14 publications

(17 citation statements)

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“…The tested ISSR primers were allowed the discovery of reasonably high level of polymorphism (average 63%) and are a reflection of presence of a wide range of genetic variation among the individuals examined. The present findings seem to be relatively similar when compared to the reports of previous ISSR studies on different tree species [32][33][34]. Our study demonstrates for the first time that the ISSR-PCR a suitable method for detection of genetic variability in sal.…”

Section: Discussionsupporting

confidence: 91%

Assessment of genetic diversity in Shorea robusta: an economically important tropical tree species

2017

J App Biol Biotech

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The present investigation reports an elucidation of genetic diversity among four-populations of most economically and ecologically important tree species, sal (Shorea robusta Gaertn.) for the first time in India, using ISSR markers. A total of one-hundred individual S. robusta trees were sampled from four different populations, considering twenty-five individuals from each population. In total, twenty-ISSR primers were screened with S. robusta DNA, and out of twenty, sixteen-primer produced reproducible amplicons. Sixteen selected ISSR markers were amplified a total of 118 alleles and the total number of amplicons for individual primers ranged from 5 to 12, with a mean of 7.37 alleles per primer, of which 74 were polymorphic with an average of 4.62 alleles per primer. The ISSR primer (GA)8YG yielded highest number of alleles (12) A dendrogram based on UPGMA analysis grouped the four populations into two major clusters, having Keonjhar population into first cluster and rest three populations into second cluster. It was notable that the second major cluster was further divided into three-separate sub-clusters, representing population from each three locations. Analysis of molecular variance (AMOVA) revealed that the majority of genetic variation exists within populations, compared to the variation that exists among the populations. The present study is a clear-cut indication that inter-and intra-population genetic variation exists in S. robusta and ISSRs appears to be an efficient marker system in quantifying genetic variability in different populations of S. robusta.

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Section: Discussionsupporting

confidence: 91%

Assessment of genetic diversity in Shorea robusta: an economically important tropical tree species

2017

J App Biol Biotech

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“…As a result, drought is by far the most complex and devastating abiotic stress on a global scale and its frequency is expected to increase with climate change (Lakew et al, 2011;Schmit, Mora, Emhart, & Rubilar, 2015). Under this scenario, the development of drought tolerantcultivars is one of the major objectives of breeding programs with a goal of reducing crop losses in agriculture (Sayed, Schumann, Pillen, Naz & Léon, 2012;Ballesta, Mora, Contreras-Soto, Ruiz & Perret, 2015).…”

Section: Introductionmentioning

confidence: 99%

<b>Identification of QTL underlying agronomic, morphological and physiological traits in barley under rainfed conditions using SNP markers

et al. 2017

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ABSTRACT. Barley (Hordeum vulgare L.) is considered a good genetic model for evaluating mechanisms of drought tolerance, and it is the most important threat to crop production worldwide. This study aimed to identify single-nucleotide polymorphisms (SNPs) associated with agronomic, morphological and physiological traits in a population of 137 recombinant chromosome substitution lines (RCSL) of barley, which were evaluated under rainfed conditions, in Cauquenes, southern Chile (35°58' S, 72°17' W). The annual precipitation was 299 mm during the growing season. Fifty-two significant QTLs were detected for the studied traits, which explained between 5% and 13.8% of the phenotypic variation. A genomic region on chromosome 1H (that comprises SNPs 2711-234 and 1923-265) accounted for 13.4% and 13.8%, respectively, of the grain yield variation. In addition, SNPs 8388-578 and 7639-122 on the chromosome 5H had a moderate effect, explaining 12.8% of the plant height variation. Moreover, some SNPs were associated with more than one trait, and clusters of QTLs for yield and related traits were also found. Finally, the QTLs identified in the present study are of particular interest for barley-breeding purposes under rainfed conditions. Keywords:Hordeum vulgare, mixed model, recombinant chromosome substitution lines, segregation distortion.Identificação de QTL associados a características agronômicas, morfológicas e fisiológicas em cevada sob condições de sequeiro, usando marcadores SNP RESUMO. Cevada (Hordeum vulgare L.) é uma cultura modelo para a avaliação genética dos mecanismos de tolerância à seca; a ameaça mais importante para a produção agrícola a nível mundial. Objetivou-se identificar polimorfismos de nucleotídeo único (SNP) associados a características agronômicas, morfológicas e fisiológicas numa população de 137 linhas recombinantes com substituição cromossômica (RCSL) de cevada, avaliadas sob condições de sequeiro, em Cauquenes, sul do Chile (35°58' S, 72°17' O). A precipitação anual foi de 299 mm durante o período vegetativo. Cinquenta e dois QTLs foram encontrados para as características estudadas, explicando entre 5% e 13.8% da variação fenotípica. A região genômica no cromossomo 1H (que compreende os SNPs 2711-234 e 1923-265) foi responsável de ~13% da variação no rendimento de grãos. Além disso, os SNPs 8388-578 e 7639-122 no cromossomo 5H teve um efeito moderado, explicando 12,8% da variação para altura da planta. Além disso, alguns SNPs foram associados com mais de uma característica, e grupos de QTLs para rendimento de grãos e características relacionadas também foram encontrados. Finalmente, os QTLs identificados no presente estudo podem ser de particular interesse para fins de melhoramento de cevada em ambiente de sequeiro.Palavras-chave: Hordeum vulgare, modelo misto, linhas recombinantes com substituição cromossômica, distorção de segregação.

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“…The results of AMOVA showed that 70% of the genetic variation occurred among all individuals, and 30% within subpopulations, and the smallest component of genetic variation was among subpopulations. Many studies on AMOVA of populations demonstrated similar conclusions, such as on E. cladocalyx and E. camaldulensis (Ballesta et al 2015, Dillon et al 2015, Mora et al 2016.…”

Section: Genetic Structurementioning

confidence: 53%

“…Some studies on other species of Eucalyptus, such as E. camaldulensis, E. marginata and E. cladocalyx also proposed a low genetic differentiation in the population, analysed by molecular markers. (Butcher et al 2002, Wheeler et al 2003, Ballesta et al 2015, Dillon et al 2015.…”

Section: Genetic Differentiationmentioning

confidence: 99%

Genetic diversity of advanced generation breeding population of Eucalyptus urophylla in China

Wh¹,

Qi²,

Lan³

et al. 2018

JTFS

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As a theoretical basis for improvement of Eucalyptus urophylla in China, the genetic diversity and structure for advanced generation breeding populations were evaluated, and the changes of genetic diversity between improved generation and their parental generation were compared. The genotyping was performed on families sampled from advanced generation with 16 neutral microsatellite loci. The testing of Hardy-Weinberg equilibrium showed significant heterozygote deficits in two improved generation breeding populations. The expected heterozygosity of the 3 rd generation was very close to that of the 2 nd generation breeding population, indicating no heterozygosity had been lost, and the genetic diversity was consistent in two improved populations supported by Shannon's information index. Wright's fixed index of two advanced generations also indicated significant heterozygote in advanced generation breeding populations, compared with their parental generation. Most of the molecular variation was explained by the source of the families, both in two advanced generations, using analysis of molecular variances (AMOVA). The advanced generation breeding population of E. urophylla had a high level of genetic diversity after heavy artificial selection, consistently, and with no significant difference among generations.

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<b>Analysis of the genetic diversity of <i>Eucalyptus cladocalyx</i> (sugar gum) using ISSR markers

Cited by 14 publications

References 31 publications

Assessment of genetic diversity in Shorea robusta: an economically important tropical tree species

Assessment of genetic diversity in Shorea robusta: an economically important tropical tree species

<b>Identification of QTL underlying agronomic, morphological and physiological traits in barley under rainfed conditions using SNP markers

Genetic diversity of advanced generation breeding population of Eucalyptus urophylla in China

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