LsrR-like protein responds to stress tolerance by regulating polysaccharide biosynthesis in Lactiplantibacillus plantarum

Meng, Fanqiang; Lyu, Yunbin; Zhao, Hongyuan; Lyu, Fengxia; Bie, Xiaomei; Lu, Yingjian; Ren, Ang; Chen, Yihua; Lu, Zhaoxin

doi:10.1016/j.ijbiomac.2022.11.180

Cited by 6 publications

(5 citation statements)

References 52 publications

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“…We previously reported that the transcriptional regulator LsrR regulated polysaccharides synthesis to respond to harsh environments . However, this could not explain maltose-enhanced bacteriocin production or the enhanced polysaccharide synthesis found in the present study.…”

Section: Introductioncontrasting

confidence: 94%

“…We previously reported that the transcriptional regulator LsrR regulated polysaccharides synthesis to respond to harsh environments. 13 However, this could not explain maltoseenhanced bacteriocin production 14 or the enhanced polysaccharide synthesis found in the present study. Therefore, polysaccharide biosynthesis clusters in L. plantarum 163 were analyzed, and transcription regulators that bind to the promoter were identified.…”

Section: ■ Introductioncontrasting

confidence: 73%

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Maltose-Enhanced Exopolysaccharide Synthesis of Lactiplantibacillus plantarum through CRP-like Protein

Meng

Lyu

Chen

et al. 2023

J. Agric. Food Chem.

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Carbon sources alter the synthesis of exopolysaccharides (EPS) in Lactiplantibacillus plantarum. Maltose increased the EPS production of L. plantarum 163 6.5-fold. Subsequently, EPS production, transcriptome, and proteome were analyzed using glucose or maltose to further clarify the regulatory mechanism. A cAMP receptor protein (UniProtKB: F9UNI5) has been identified to control EPS synthesis in the presence of cAMP by binding to the EPS synthesis promoter Pcps4A‑J. Overexpression of the cAMP synthesis gene cyaA increased cAMP content and EPS production 4.5- and 2.2-fold, respectively. Furthermore, yogurt produced with L. plantarum 163-cyaA had a similar viscosity to that of commercial Greek yogurt; it had 20 and 83.7% greater viscosity than that produced with L. plantarum 163 with maltose and glucose, respectively. These findings indicated that L. plantarum 163-cyaA has potential applications in the production of functional fermented dairy products.

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Section: Introductioncontrasting

confidence: 94%

Section: ■ Introductioncontrasting

confidence: 73%

Maltose-Enhanced Exopolysaccharide Synthesis of Lactiplantibacillus plantarum through CRP-like Protein

Meng

Lyu

Chen

et al. 2023

J. Agric. Food Chem.

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“…A number of heat shock proteins were also identified, indicating the heat tolerance of B. velezensis SH-1471 44 . In addition, we have included genes encoding general stress response proteins, DNA repair proteins, cell wall integrity, and stress response components in the B. velezensis SH-1471 genome that help the strain cope with harsh environments 45 , 46 .…”

Section: Discussionmentioning

confidence: 99%

Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants

Shen,

Shi,

Zhao

et al. 2023

Sci Rep

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Bacillus is widely used in agriculture due to its diverse biological activities. We isolated a Bacillus velezensis SH-1471 from the rhizosphere soil of healthy tobacco, which has broad-spectrum antagonistic activity against a variety of plant pathogenic fungi such as Fusarium oxysporum, and can be colonized in the rhizosphere of a variety of plants. This study will further explore its mechanism by combining biological and molecular biology methods. SH-1471 contains a ring chromosome of 4,181,346 bp with a mean G + C content of 46.18%. We identified 14 homologous genes related to biosynthesis of resistant secondary metabolite, and three clusters encoded potential new antibacterial substances. It also contains a large number of genes from colonizing bacteria and genes related to plant bacterial interactions. It also contains genes related to environmental stress, as well as genes related to drug resistance. We also found that there are many metabolites in the strain that can inhibit the growth of pathogens. In addition, our indoor pot test found that SH-1471 has a good control effect on tomato wilt, and could significantly improve plant height, stem circumference, root length, root weight, and fresh weight and dry weight of the aboveground part of tomato seedlings. Therefore, SH-1471 is a potential biological control strain with important application value. The results of this study will help to further study the mechanism of SH-1471 in biological control of plant diseases and promote its application.

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“…Currently, there is a greater emphasis on comprehensive investigations of constructed wetlands with submerged plants, both domestically and internationally [10]. Conversely, research on submerged-plant-based constructed wetlands primarily focuses on the removal mechanisms of pollutants by attached biofilms [15,16]; intramembrane microbial community structures [10]; external environmental conditions such as microcystins [17], microplastics [18], antibiotics [19], and harmful algal bloom harvests [20]; and other factors in the structure of biofilm microbial communities . However, limited attention has been paid to measures aimed at enhancing the removal efficiency of submerged-plant-based constructed wetlands, which hinders their widespread adoption and application.…”

Section: Introductionmentioning

confidence: 99%

“…The Vallisneria exhibits well-developed root tissue, strong pollution resistance, high reproductive capacity, low light tolerance, and effective removal of ammonia nitrogen [21]. Vallisneria natans is a common submerged macrophyte in most eutrophic lakes in China that can tolerate and purify polluted water [17]. Therefore, this study used the epiphytic biofilm on grass leaves as its research object, focusing on investigating the wastewater treatment efficiency of a constructed wetland with submerged plants and different air-water ratios.…”

Section: Introductionmentioning

confidence: 99%

Impact of Gas-to-Water Ratio on Treatment Efficiency of Submerged-Macrophyte Constructed Wetland Systems

Mao,

Lu,

Huang

et al. 2024

Water

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Constructed wetland systems employing submerged macrophytes are increasingly utilized for treating municipal and industrial wastewater, as well as odoriferous and eutrophic water bodies. However, the pollutant removal efficiency of these systems needs further enhancement. In this study, we examined the impact of the gas-to-water ratio on the treatment efficiency of the constructed wetland of Vallisneria. We also examined the extracellular polymeric substances (EPSs) of the floating biofilm and the structure of the microbial community in this system. Our findings showed that the gas-to-water ratio significantly affects the total nitrogen (TN) removal rate within the Vallisneria wetlands, with an optimum removal at a gas-to-water ratio of 15:1, while the removal efficiencies for chemical oxygen demand (COD), NH4+-N, and total phosphorus (TP) remain relatively unaffected. Increased gas-to-water ratios corresponded to a notable decrease in biofilm EPSs. High-throughput sequencing analysis demonstrated a shift in biofilm-denitrifying bacteria from anoxic heterotrophic to aerobic denitrifiers, alongside a significant rise in the abundance of denitrifying bacteria, whereas excessively high gas-to-water ratios inhibited the growth of these bacteria. A gas-to-water ratio of 15:1 constituted the optimal condition for ecological restoration of the water body within the Vallisneria wetland systems. These results could contribute to the optimization of submerged-macrophyte constructed wetland system design and the enhancement of treatment efficiency.

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LsrR-like protein responds to stress tolerance by regulating polysaccharide biosynthesis in Lactiplantibacillus plantarum

Cited by 6 publications

References 52 publications

Maltose-Enhanced Exopolysaccharide Synthesis of Lactiplantibacillus plantarum through CRP-like Protein

Maltose-Enhanced Exopolysaccharide Synthesis of Lactiplantibacillus plantarum through CRP-like Protein

Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants

Impact of Gas-to-Water Ratio on Treatment Efficiency of Submerged-Macrophyte Constructed Wetland Systems

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