Lsi1 plays an active role in enhancing the chilling tolerance of rice roots

Zhong, Li; Feng, Shizhong; Zhan, Wenshan; Xu, Linlin; Fang, Changxun; Zhang, Zhixing; Lin, Wenxiong

doi:10.1007/s10725-020-00577-5

Cited by 9 publications

(10 citation statements)

References 45 publications

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“…Rice root proteins have been studied using iTRAQ technology under CS [139]. The study reveals a total of 433 DAPs.…”

Section: Proteomics: Can Proteins Make It Happen?mentioning

confidence: 99%

“…Results revealed that Lsi1 (low silicon rice 1) and overexpressing (Lsi1-OX) identified enhanced ability of the antioxidant system and signaling pathway at the physiological level. Besides, improving rice capability to regulate interconnected signals stimulating biochemical processes under CS [139]. Cold stress regulation networks acknowledged under proteomic study using iTRAQ in peanut (Arachis hypogaea L.) buds.…”

Section: Proteomics: Can Proteins Make It Happen?mentioning

confidence: 99%

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Can omics deliver temperature resilient ready-to-grow crops?

Raza

Tabassum

Kudapa

et al. 2021

Critical Reviews in Biotechnology

132

110

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Plants are extensively well-thought-out as the main source for nourishing natural life on earth. In the natural environment, plants have to face several stresses, mainly heat stress (HS), chilling stress (CS) and freezing stress (FS) due to adverse climate fluctuations. These stresses are considered as a major threat for sustainable agriculture by hindering plant growth and development, causing damage, ultimately leading to yield losses worldwide and counteracting to achieve the goal of "zero hunger" proposed by the Food and Agricultural Organization (FAO) of the United Nations. Notably, this is primarily because of the numerous inequities happening at the cellular, molecular and/or physiological levels, especially during plant developmental stages under temperature stress. Plants counter to temperature stress via a complex phenomenon including variations at different developmental stages that comprise modifications in physiological and biochemical processes, gene expression and differences in the levels of metabolites and proteins. During the last decade, omics approaches have revolutionized how plant biologists explore stress-responsive mechanisms and pathways, driven by current scientific developments. However, investigations are still required to explore numerous features of temperature stress responses in plants to create a complete idea in the arena of stress signaling. Therefore, this review highlights the recent advances in the utilization of omics approaches to understand stress adaptation and tolerance mechanisms. Additionally, how to overcome persisting knowledge gaps. Shortly, the combination of integrated omics, genome editing, and speed breeding can revolutionize modern agricultural production to feed millions worldwide in order to accomplish the goal of "zero hunger." ARTICLE HISTORY

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“…Rice root proteins have been studied using iTRAQ technology under CS [139]. The study reveals a total of 433 DAPs.…”

Section: Proteomics: Can Proteins Make It Happen?mentioning

confidence: 99%

Section: Proteomics: Can Proteins Make It Happen?mentioning

confidence: 99%

Can omics deliver temperature resilient ready-to-grow crops?

Raza

Tabassum

Kudapa

et al. 2021

Critical Reviews in Biotechnology

132

110

View full text Add to dashboard Cite

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“…The total protein from the rice leaves was extracted using the trichloroacetic acid (TCA)/acetone method [44]. Briefly, a mortar precooled in liquid nitrogen with a small amount of quartz sand was used to grind 2 g of frozen (−80 • C) leaf samples from the Dular-WT and Dular-OE lines.…”

Section: Protein Extraction and Western Blot Analysismentioning

confidence: 99%

“…A total of 1 g of fresh plant leaves (three biological repeats) treated at a low and normal temperature for 48 h were added to 2 mL of precooled acetone to prepare the homogenate. This was centrifuged at 3000× g for 10 min, and the supernatant was used for estimating the H 2 O 2 content by measuring the absorbance at 415 nm as described by Li et al, [44]. We first measured the enzyme activities, chlorophyll content, proline, and H 2 O 2 in fresh weight.…”

Section: Determination Of Chlorophyll H 2 O 2 Enzyme Activity and Pr...mentioning

confidence: 99%

Deciphering the Molecular Mechanisms of Chilling Tolerance in Lsi1-Overexpressing Rice

Khan

Xue

et al. 2022

IJMS

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Improving tolerance to low-temperature stress during the rice seedling stage is of great significance in agricultural science. In this study, using the low silicon gene 1 (Lsi1)-overexpressing (Dular-OE) and wild-type rice (Dular-WT), we showed that Lsi1 overexpression enhances chilling tolerance in Dular-OE. The overexpression of the Lsi1 increases silicon absorption, but it was not the main reason for chilling tolerance in Dular-OE. Instead, our data suggest that the overexpression of a Lsi1-encoding NIP and its interaction with key proteins lead to chilling tolerance in Dular-OE. Additionally, we show that the high-mobility group protein (HMG1) binds to the promoter of Lsi1, positively regulating its expression. Moreover, Nod26-like major intrinsic protein (NIP)’s interaction with α and β subunits of ATP synthase and the 14-3-3f protein was validated by co-immunoprecipitation (Co-IP), bimolecular fluorescent complementary (BiFC), and GST-pulldown assays. Western blotting revealed that the overexpression of NIP positively regulates the ATP-synthase subunits that subsequently upregulate calcineurin B-like interacting protein kinases (CIPK) negatively regulating 14-3-3f. Overall, these NIP-mediated changes trigger corresponding pathways in an orderly manner, enhancing chilling tolerance in Dular-OE.

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“…Dular was a landrace aus variety that originated from India [32,33]. Dular exhibited significant chilling injury at temperatures below 15 • C [34,35]. The damage to plants under chilling is closely related to the intensity and time of stress.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of the Responses of Rice Leaves to Chilling and Subsequent Recovery

Khan

Letuma

et al. 2022

IJMS

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Improving chilling tolerance at the seedling stage in rice is essential for agricultural research. We combined a physiological analysis with transcriptomics in a variety Dular subjected to chilling followed by recovery at normal temperature to better understand the chilling tolerance mechanisms of rice. Chilling inhibited the synthesis of chlorophyll and non-structural carbohydrate (NSC) and disrupted the ion balance of the plant, resulting in the impaired function of rice leaves. The recovery treatment can effectively reverse the chilling-related injury. Transcriptome results displayed that 21,970 genes were identified at three different temperatures, and 11,732 genes were differentially expressed. According to KEGG analysis, functional categories for differentially expressed genes (DEGs) mainly included ribosome (8.72%), photosynthesis–antenna proteins (7.38%), phenylpropanoid biosynthesis (11.41%), and linoleic acid metabolism (10.07%). The subcellular localization demonstrated that most proteins were located in the chloroplasts (29.30%), cytosol (10.19%), and nucleus (10.19%). We proposed that some genes involved in photosynthesis, ribosome, phenylpropanoid biosynthesis, and linoleic acid metabolism may play key roles in enhancing rice adaptation to chilling stress and their recovery capacity. These findings provide a foundation for future research into rice chilling tolerance mechanisms.

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Lsi1 plays an active role in enhancing the chilling tolerance of rice roots

Cited by 9 publications

References 45 publications

Can omics deliver temperature resilient ready-to-grow crops?

Can omics deliver temperature resilient ready-to-grow crops?

Deciphering the Molecular Mechanisms of Chilling Tolerance in Lsi1-Overexpressing Rice

Transcriptome Analysis of the Responses of Rice Leaves to Chilling and Subsequent Recovery

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