LPS Binding Protein (LBP) and Serum Amyloid A (SAA) are released by human intestinal epithelial cells in response to cytokines

Vreugdenhil, Anita; Dentener, Mieke A.; Aben, Joris A.; Greve, J.W.M.; Buurman, Wim A.

doi:10.1097/00042737-199812000-00245

Cited by 81 publications

(104 citation statements)

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“…Besides detoxification of toxin in the circulation by LBP-chylomicron complexes, it can be hypothesized that this mechanism may be part of a local defense mechanism of the intestine against translocated bacterial toxin. Chylomicrons are produced by enterocytes, and interestingly, recent evidence was found for the basolateral secretion of LBP by the intestinal epithelium in response to cytokines (35). It is tempting to speculate from the data presented here that LBP and chylomicrons, both secreted by the intestinal mucosa, cooperate in local neutralization of LPS.…”

Section: Figurementioning

confidence: 50%

Lipopolysaccharide (LPS)-Binding Protein Mediates LPS Detoxification by Chylomicrons

Vreugdenhil

Rousseau

Hartung

et al. 2003

The Journal of Immunology

185

132

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Chylomicrons have been shown to protect against endotoxin-induced lethality. LPS-binding protein (LBP) is involved in the inactivation of bacterial toxin by lipoproteins. The current study examined the interaction among LBP, chylomicrons, and bacterial toxin. LBP was demonstrated to associate with chylomicrons and enhance the amount of LPS binding to chylomicrons in a dose-dependent fashion. In addition, LBP accelerated LPS binding to chylomicrons. This LBP-induced interaction of LPS with chylomicrons prevented endotoxin toxicity, as demonstrated by reduced cytokine secretion by PBMC. When postprandial circulating concentrations of chylomicrons were compared with circulating levels of low density lipoprotein, very low density lipoprotein, and high density lipoprotein, chylomicrons exceeded the other lipoproteins in LPS-inactivating capacity. Furthermore, highly purified lipoteichoic acid, an immunostimulatory component of Gram-positive bacteria, was detoxified by incubation with LBP and chylomicrons. In conclusion, our results indicate that LBP associates with chylomicrons and enables chylomicrons to rapidly bind bacterial toxin, thereby preventing cell activation. Besides a role in the detoxification of bacterial toxin present in the circulation, we believe that LBP-chylomicron complexes may be part of a local defense mechanism of the intestine against translocated bacterial toxin.

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Section: Figurementioning

confidence: 50%

Lipopolysaccharide (LPS)-Binding Protein Mediates LPS Detoxification by Chylomicrons

Vreugdenhil

Rousseau

Hartung

et al. 2003

The Journal of Immunology

185

132

View full text Add to dashboard Cite

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“…Lipopolysaccharide-binding protein (LBP) is an acute phase protein markedly induced during inflammation and infection (23,24). Its major site of synthesis is the liver, but LBP is also produced in the intestine and lungs, where it may play a role in local responses to bacterial lipopolysaccharide (25,26). Serum amyloids (SAA1/2) are multifunctional acute phase proteins possessing both pro-and anti-inflammatory activities that can increase 1,000-fold in the blood in response to inflammation and infection (27).…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Patterns in the Human Breast after Pregnancy

Asztalos

Gann

Hayes

et al. 2010

Cancer Prevention Research

118

117

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Epidemiologic studies have established that pregnancy has a bidirectional, time-dependent effect on breast cancer risk; a period of elevated risk is followed by a long-term period of protection. The purpose of the present study was to determine whether pregnancy and involution are associated with gene expression changes in the normal breast, and whether such changes are transient or persistent. We examined the expression of a customized gene set in normal breast tissue from nulliparous, recently pregnant (0-2 years since pregnancy), and distantly pregnant (5-10 years since pregnancy) age-matched premenopausal women. This gene set included breast cancer biomarkers and genes related to immune/inflammation, extracellular matrix remodeling, angiogenesis, and hormone signaling. Laser capture microdissection and RNA extraction were done from formalin-fixed paraffin-embedded reduction mammoplasty and benign biopsy specimens and analyzed using real-time PCR arrays containing 59 pathway-specific and 5 housekeeping genes. We report 14 of 64 (22%) of the selected gene set to be differentially regulated (at P < 0.05 level) in nulliparous versus parous breast tissues. Based on gene set analysis, inflammationassociated genes were significantly upregulated as a group in both parous groups compared with nulliparous women (P = 0.03). Moreover, parous subjects had significantly reduced expression of estrogen receptor α (ERα, ESR1), progesterone receptor (PGR), and ERBB2 (Her2/neu) and 2-fold higher estrogen receptor-β (ESR2) expression compared with nulliparous subjects. These initial data, among the first on gene expression in samples of normal human breast, provide intriguing clues about the mechanisms behind the time-dependent effects of pregnancy on breast cancer risk. Cancer Prev Res; 3(3); 301-11. ©2010 AACR.

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“…SAA1 is well-known as an acute reactive protein primarily derived from the liver. The function of SAA1 produced locally in various epithelia including intestine 18,19 remains to be elucidated. Measuring MRP14…”

Section: Discussionmentioning

confidence: 99%

Inflammatory gene signature in ulcerative colitis with cDNA macroarray analysis

Okahara

Arimura

Yabana³

et al. 2005

Aliment Pharmacol Ther

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SUMMARYBackground: Most array analyses of ulcerative colitis have focused on identifying susceptibility genes for ulcerative colitis. Aim: To clarify the changes in gene expression during inflammation in ulcerative colitis colon mucosa using cDNA macroarray. Methods: From 23 ulcerative colitis patients, 16 each of inflamed and non-inflamed specimens (total 32 samples for individual analysis) were obtained by colonoscopic biopsy. Eighteen of the 32 samples, used for pairwise analysis, consisted of nine sample pairs, each pair being from the same patient. We examined expression profiles of approximately 1300 genes with cDNA macroarray.

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LPS Binding Protein (LBP) and Serum Amyloid A (SAA) are released by human intestinal epithelial cells in response to cytokines

Cited by 81 publications

References 0 publications

Lipopolysaccharide (LPS)-Binding Protein Mediates LPS Detoxification by Chylomicrons

Lipopolysaccharide (LPS)-Binding Protein Mediates LPS Detoxification by Chylomicrons

Gene Expression Patterns in the Human Breast after Pregnancy

Inflammatory gene signature in ulcerative colitis with cDNA macroarray analysis

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