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Shimizu, Kayoko

doi:10.3925/jjtc1958.27.559

Cited by 28 publications

(45 citation statements)

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“…Using HeLa cell nuclear extracts and short synthetic RNA molecules, it was demonstrated that these cells have the ability to join RNA segments from two different precursor molecules together by trans-splicing (Konarska et al, 1985;Solnick, 1985). Furthermore, cDNA sequencing experiments supported the proposal that trans-splicing might account for mRNA generation in vivo, but alternative cis-splicing could not be entirely excluded Shimizu et al, 1991;Sullivan et al, 1991).…”

Section: Introductionmentioning

confidence: 97%

Experimental evidence for RNA trans-splicing in mammalian cells.

Eul

Graessmann

1995

The EMBO Journal

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We present evidence that mammalian cells have the ability to generate functional mRNA molecules by trans‐splicing. Rat cells, transformed by an early SV40 DNA fragment (Bst/Bam) synthesize a truncated T antigen (T1 antigen), although the cells do not have a direct sequence homology for the T1 antigen at the DNA level. The Bst/Bam DNA fragment encodes exclusively for the second SV40 T antigen exon (aa 83‐708) and contains the entire small t antigen intron. To synthesize the corresponding mRNA (T1 mRNA), the cells utilize a cryptic 5′ splice site within the second exon (codons for aa 131/132) as donor site and the upstream small t antigen 3′ splice site as the acceptor site. Since these sites are in an inverted order on the pre‐mRNA, two Bst/Bam transcripts are required to generate one T1 mRNA molecule. HeLa cell nuclear extracts also performed the trans‐splicing reaction in vitro.

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Section: Introductionmentioning

confidence: 97%

Experimental evidence for RNA trans-splicing in mammalian cells.

Eul

Graessmann

1995

The EMBO Journal

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“…Porcine astrovirus (PAstV) was first detected by EM in the feces of a diarrheic piglet [6] and was later isolated in culture [18]. Molecular characterization of the capsid (ORF2) gene from this isolate followed some years later [19].…”

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confidence: 99%

“…To investigate the relationship between Astroviruses and other economically important viral diseases that cause diarrhea in piglets in Asia, screening tests were conducted to detect Porcine Epidemic Diarrhea Virus (PEDV), Transmissible Gastroenteritis Virus (TGEV), and Porcine Group A Rotavirus (GAR), as previously described [25]. The primer pairs used in this study were P1 (TTCTGA GTCA CGAACAGCCA, 1466-1485) and P2 (CATATG CAGCCTGCTCTGAA, 2097-2116) for the S gene of PEDV, T1 (GTGGTTTTGGTYRTAAATGC, [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] and T2 (CACTAACCAACGTGGARCTA, 855-874) for the S gene of TGEV, and rot3 (AAAGATGCTAGGGACAAA ATTG, 57-78) and rot5 (TTCAGATTGT GGAGCTA TTCCA, 344-365) for the segment 6 region of group A Rotavirus. The sizes of the expected products of multiplex RT-PCR were 859 bp for TGEV, 651 bp for PEDV, and 309 bp for rotavirus, which could be differentiated by agarose gel electrophoresis.…”

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confidence: 99%

“…The clinical symptoms of diarrhea are frequently reported to be associated with Rotavirus, Coronavirus, and Calicivirus-like infections in piglets [6,18,20,21,26]. Although PEDV and TGEV infections were not identified in any of the fecal samples, porcine GAR infection was identified in 6.2 % (17/275) of the samples collected from suckling pigs under 3 weeks old (Table 2).…”

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Phylogenetic analysis of porcine astrovirus in domestic pigs and wild boars in South Korea

et al. 2012

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Porcine astrovirus (PAstV) belongs to genetically divergent lineages within the genus Mamastrovirus. In this study, 25/129 (19.4 %) domestic pig and 1/146 (0.7 %) wild boar fecal samples tested in South Korea were positive for PAstV. Positive samples were mainly from pigs under 6 weeks old. Bayesian inference (BI) tree analysis for RNA-dependent RNA polymerase (RdRp) and capsid (ORF2) gene sequences, including Mamastrovirus and Avastrovirus, revealed a relatively geographically divergent lineage. The PAstVs of Hungary and America belong to lineage PAstV 4; those of Japan belong to PAstV 1; and those of Canada belong to PAstV 1, 2, 3, and 5, but not to 4. This study revealed that the PAstVs of Korea belong predominantly to lineage PAstV 4 and secondarily to PAstV 2. It was also observed that PAstV infections are widespread in South Korea regardless of the disease state in domestic pigs and in wild boars as well.

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“…It also improves the voice and applied to burns. Leaves yield â-carotene in large amount and á-carotene in small amounts and traces of other carotenoids 5 , Pro-vitamin A 6,7 , Lipids, amino acids, protein 8,9 and ascorbic acid 10 , Cao et. al 11 extracted a red pigment from the fruit of Basella rubra which has good colouring ability.…”

Section: Introductionmentioning

confidence: 99%

Structural Elucidation of two Compounds from the Fruit of Medicinally Important Plant, Basella rubra

Choudhury¹,

De²,

Paul³

2016

Orient. J. Chem

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Basella rubra is a medicinal plant species found in India and other parts of Asia and tropical Africa. It improves the appetite, useful in biliousness, leprosy, dysentery, ulcers and it has antiviral and antipyretic activity. The red pigment from the fruit has good colouring ability. It is bright purple at pH 3-7. It is a good additive and a safe, non-poisonous colorant. At present, the dyestuff industry has grown rapidly because of the vital role played by small -scale sector, which is responsible for the production of azo, acids, basics and direct dyes. But such synthetic dyes have toxic effect and hence may not be useful in edible substances. Therefore keeping this in mind the structure of two separated compounds from the fruit pigment of medicinally important plant Besella rubra were elucidated. The structures of the compounds have been characterized on the basis of spectral data such as IR, 1 H NMR, 13 C NMR and Mass spectral studies.

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Cited by 28 publications

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Experimental evidence for RNA trans-splicing in mammalian cells.

Experimental evidence for RNA trans-splicing in mammalian cells.

Phylogenetic analysis of porcine astrovirus in domestic pigs and wild boars in South Korea

Structural Elucidation of two Compounds from the Fruit of Medicinally Important Plant, Basella rubra

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