LOX gene polymorphisms are associated with osteoporotic vertebral compression fracture in postmenopausal Chinese women

Xiao, Wenjin; He, Jia; Fu, Wen‐Zhen; Xu, Youjia; Zhang, Zhenlin

doi:10.1016/j.gene.2020.144543

Cited by 7 publications

(8 citation statements)

References 36 publications

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“…Therefore, LOX G473A is an important gene locus that affects human health. Recently, we confirmed for the first time that LOX G473A is closely related to lumbar fractures in postmenopausal women (17). Compared with the GG genotype, the fracture risk of people with GA and AA genotypes increased by 1.28-and 1.74-fold, respectively (17).…”

Section: Introductionmentioning

confidence: 62%

“…Recently, we confirmed for the first time that LOX G473A is closely related to lumbar fractures in postmenopausal women (17). Compared with the GG genotype, the fracture risk of people with GA and AA genotypes increased by 1.28-and 1.74-fold, respectively (17). Nevertheless, the mechanism by which LOX G473A drives osteoclast differentiation remains vague in osteoporosis.…”

Section: Introductionmentioning

confidence: 71%

“…Research has shown that kaempferol restrains bone resorption and osteoclast differentiation by inhibiting autophagy (20). Based on the previously known involvement of LOX G473A in the regulation of osteoclast formation (17), and the role of autophagy in the regulation of osteoclast formation (20), it is not clear whether they have a reciprocal regulatory relationship. In this study, the pre-osteoclast cell line RAW264.7 were used to explore the role of LOX G473A in osteoclast differentiation and the potential role of autophagy in osteoclast differentiation, giving insight to the treatment of osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

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Induction of osteoclast formation by LOX mutant (LOXG473A) through regulation of autophagy

Zhang

Luo²,

Xiao

2021

Ann Transl Med

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Background: Lysyl oxidase (LOX) has been identified to modulate osteoclast activity, so we explored the role of LOX G473A , the highest frequency single nucleotide polymorphism in LOX, in osteoclast formation and its potential relationship to autophagy. Methods:The ability of the LOX mutant, LOX G473A , to promote autophagy and osteoclast formation was evaluated in the pre-osteoclast cell line RAW264.7. Furthermore, autophagy-related protein expression and autophagosomes were detected by western blot and electron microscopy, respectively. Simultaneously, osteoclast formation and resorption ability were also detected using TRAP staining assay and bone resorption assay. In addition, the osteoclast-related proteins and mRNAs, as well as p-AMPKα and p-mTOR proteins, were further evaluated by western blot and qPCR assays.Results: Autophagy inhibitor 3-MA suppressed the Beclin-1 and ATG5 protein levels and the ratio of LC3-II to LC3-I, as well as autophagosome formation in RAW264.7 transfected with the MUT plasmid and enhanced p62 protein expression. Simultaneously, 3-MA also reduced osteoclast formation and resorption, as well as the F-actin ring level of osteoclasts. In addition, 3-MA inhibited osteoclast-related protein and mRNA expression, including NFATC1, ACP5, CTSK. And the autophagy-related pathway protein p-AMPKα was increased and p-mTOR was reduced by 3-MA treatment. However, autophagy agonist RAPA reversed the effect of 3-MA on RAW264.7 with LOX G473A mutation, indicating that promoting autophagy could enhance the ability of LOX G473A to induce osteoclast formation.Conclusions: LOX mutant (LOX G473A ) might promote osteoclast formation for RAW264.7 by enhancing autophagy via the AMPK/mTOR pathway, which is a new direction for bone disease research.

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Section: Introductionmentioning

confidence: 62%

Section: Introductionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

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Induction of osteoclast formation by LOX mutant (LOXG473A) through regulation of autophagy

Zhang

Luo²,

Xiao

2021

Ann Transl Med

Self Cite

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“…Due to the social aging, the incident rate of osteoporotic vertebral compression fracture has increased year by year with its insidious onset and low consultation rate seriously threatening the health of the elderly, which is more signi cant in postmenopausal women [1][2][3]. In view of the actual clinical problems, researches on OVCF have become urgent and necessary [4,5].…”

Section: Introductionmentioning

confidence: 99%

Study on the Therapeutic Effect of ESPB for Patients Undergoing Percutaneous Vertebroplasty

Peng

Jiang

2022

Preprint

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Objective To compare the difference oftherapeutic effect between percutaneous vertebroplasty (PVP)alone and PVP + Erector spinal plane block (ESPB) in the treatment of osteoporotic vertebral compression fracture (OVCF). Methods After admission, 100 patients with osteoporotic vertebral compression fracture were randomly divided into PVP group (control group) and PVP+ESPB group (observation group) by lottery with 50 patients in each group.The visual analog pain scores (VAS) and oswestry disability index (ODI) of each group were compared before surgery, 2 hours after surgery and at the time of hospital discharge. The operation time, filling volume of intraoperative bone cement, intraoperative bloodloss, and surgery cost of each group were compared.Theearly postoperative ambulation time and early postoperative defecation (stool) time ofeach group were compared to explore the difference.Results The PVP+ESPB group had better visual analog pain scores (VAS) and oswestry disability index (ODI) at 2 hours after surgery and at the time of hospital discharge, and better early postoperative ambulation timeand early postoperative defecation (stool) timethan the PVP group (p<0.05). There were no differences in the visual analog pain scoresand oswestry disability index before surgery, operative time, filling volume of intraoperative bone cement, intraoperative blood loss, and surgery cost between the two groups, and no complications occurred between the two groups after surgery and at the time of hospital discharge. Conclusion PVP+ESPB for OVCF is associated with lower VAS, more significant pain relief, and lower oswestry disability index in patients after surgery than PVP alone for OVCF, and patients are able to engage in ambulation time earlier. Meanwhile, PVP+ESPB promotes recovery of intestinalfunction and improves patients’ quality of life so as tocontribute toquick recovery.

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“…Few studies about innovative popular science models and their effects have been retrieved. In addition, because osteoporosis (OP) became important health problem for people over 50 years old in China, especially for elderly women [4][5][6] , and the public still knows little about it, OP prevention and control is the issue in this study.…”

Section: Introductionmentioning

confidence: 99%

Impact of Different Popular Science Models and Other Factors on Satisfaction of Community Health Popular Science Activities in Shanghai Community Residents

Ping

2021

Preprint

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Objective To explore and analyze the impact of different popular science models, include traditional and innovative models, and other factors on satisfaction of community health popular science activities in Shanghai community residents. Method Four communities were selected in Minhang District, Shanghai, and divided into four groups with simple random sampling method: foreign volunteer medical science talk show group (A), Chinese volunteer medical science talk show group (B), traditional lecture group (C), and control group (D). Using the rules of convenience sampling, 150 residents were randomly selected from each community to participate in 6-month popular science activities, and completed three questionnaires during the 6-month popular science intervention. Data including the sociodemographic data and the satisfaction on community health popular science activities was collected and analyzed. Results Compared with those in baseline, the satisfactions of community health popular science activities in three popular science models have all significantly improved with 3 and 6 months popular science intervention (A: χ2 = 47.1, p < 0.001; B: χ2 = 62.0, p < 0.001; C: χ2 = 31.7, p < 0.001) except for in control group (D: χ2 = 0.3, p = 0.9). The factors including gender, education, marital status, popular science model and popular science intervention time all have significant impact on satisfaction. Among these factors, married (Exp (B) = 2.715, 95% CI (1.163-6.336), p = 0.021)), popular science models (foreign volunteer medical science talk show model: Exp (B) = 8.796, 95% CI (5.907-13.100), p < 0.001; Chinese volunteer medical science talk show model: Exp (B) = 5.106, 95% CI (3.564-7.314), p < 0.001 and traditional lecture model: Exp (B) = 13.033, 95% CI (8.374-20.283), p < 0.001), popular science intervention time (3-month: Exp (B) = 3.988, 95% CI (2.827-5.626), p < 0.001; 6-month: Exp (B) = 4.132, 95% CI (2.924-5.840), p < 0.001) have positive impact on satisfaction. While female (Exp (B) = 0.598, 95% CI (0.440-0.813), p = 0.001), and junior college (Exp (B) = 0.295, 95% CI (0.168-0.519), p < 0.001) have negative impact. Conclusion Both the innovative and traditional popular science models have positive effect on the satisfaction on Chinese community health popular science activities in Shanghai community residents. In the future, special consideration should be given to the impact of different popular science models and other factors on the popular science effects including the satisfaction, and more tailor-design health popular science activities will be suitable for community residents.

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LOX gene polymorphisms are associated with osteoporotic vertebral compression fracture in postmenopausal Chinese women

Cited by 7 publications

References 36 publications

Induction of osteoclast formation by LOX mutant (LOXG473A) through regulation of autophagy

Induction of osteoclast formation by LOX mutant (LOXG473A) through regulation of autophagy

Study on the Therapeutic Effect of ESPB for Patients Undergoing Percutaneous Vertebroplasty

Impact of Different Popular Science Models and Other Factors on Satisfaction of Community Health Popular Science Activities in Shanghai Community Residents

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