Lowered Resistance to Iron in Vitamin-E Deficient Piglets and Mice

The Influence of Diet on the Acute Toxicity of Injectable Iron Preparations in the Mouse

“…The above results confirm and extend the observations of Lannek, Lindberg who showed that in animals maintained on Vitamin E-deficient diets the toxicity of the iron-dextran preparation Imposil was increased. The results we have obtained are more striking since they were based on intravenous toxicity figures but we have also found that the intraperitoneal toxicity of Imposil in mice on a Vitamin E-deficient diet prepared according to the method of Friedman, Weiss, Wherry & Kline (1958) is 860 mg Fe/kg whereas on Diet 41B it is about 2,000 mg Fe/kg, these figures agreeing closely with those published by Lannek, Lindberg & Tollerz (1962). In a subsequent paper Tollerz & Lannek (1964) showed that even the intraperitoneal LD50 of Imposil could be reduced to as low as 100 mg Fe/kg if the mice were maintained on the Vitamin E-deficient diet for up to six weeks rather than for three weeks.…”

“…Nevertheless it is evident that the intravenous route provides a more sensitive method for the detection of such changes in toxicity. Initially Lannek, Lindberg & Tollerz (1962) protected mice against the toxic effects of iron by pre-treatment with a-tocopherol acetate intramuscularly twice a week during the twenty-one days before the LD50 determination; the dose corresponded to about 7 mg/kg per day. In our experiments the daily intake of a-and f-tocopherols from Oxoid Breeding Diet was approximately 7.5 mg/kg and thus protection can be achieved with a rather lower total dose of Vitamin E administered by injection.…”

Cholestatic hepatopathy, thrombocytopenia and lymphopenia associated with iron toxicity in a Thoroughbred gelding

“…In other species, underlying hepatic disease augments the risk of intoxication subsequent to iron administration ( Ruhr 1983). Vitamin E deficiency in piglets is associated with an increased rate of toxicity and mortality following iron administration (Lannek et al 1962;Tollerz and Lanneck 1964;Arpi and Tollerz 1965). Toxicity is prevented in these piglets when vitamin E or other antioxidants are administered before iron supplementation.…”

Dietary Iron in Swine Rations Affects Nonheme Iron and TBARS in Pork Skeletal Muscles

“…Cooked RF on day 12 revealed a faster rate of tocopherol degradation in RF from the high-iron group, suggesting the need for more a-tocopherol to quench free radicals in lipid membranes from the high-iron treatment. Vitamin E is destroyed in vivo when excess iron is consumed (Lannek et al, 1962).…”