Lower doses of vanadate in combination with trigonella restore altered carbohydrate metabolism and antioxidant status in alloxan-diabetic rats

Mohamad, Sameer; Taha, Asia; Bamezai, R.; Basir, Seemi Farhat; Baquer, Najma Zaheer

doi:10.1016/j.cccn.2003.12.005

Cited by 48 publications

(38 citation statements)

References 40 publications

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“…Values followed by different letters (a, b) indicate significant differences between the groups at the indicated level P<0.05 (ANOVA/Tukey's test) a Two-way analysis of variance (ANOVA; test F; P<0.05). NS, no significant effect Ścibior and Zaporowska 1998;Ścibior and Zaporowska 2002;Ścibior 2005) and in the studies of other authors (Meyerovitch et al 1989, Russanov et al 1994, Dai et al 1995, Poggioli et al 2001, Thompson et al 2002, Mohamad et al 2004, Kordowiak et al 2005, Dąbroś et al 2006). However, other investigators did not demonstrate any significant changes in the diet and fluid intake (Bishayee and Chatterjee 1995;De Tata et al 2000;Poggioli et al 2001) or in body weight gain (Dai et al 1995;Thompson et al 2002) after treating rats with vanadate or vanadyl in drinking water.…”

Section: Discussionmentioning

confidence: 79%

Antioxidant enzyme activity and lipid peroxidation in the blood of rats co-treated with vanadium (V+5) and chromium (Cr+3)

et al. 2010

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Selected biochemical parameters were studied in the blood of outbred, male Wistar rats which daily received to drink deionized water (Group I, control) or solutions of: sodium metavanadate (SMV; 0.100 mg V/mL)-Group II; chromium chloride (CC; 0.004 mg Cr/mL)-Group III; and SMV-CC (0.100 mg V and 0.004 mg Cr/mL)-Group IV for a 12-week period. The diet and fluid intake, body weight gain, and food efficiency ratio (FER) diminished significantly in the rats of Groups II and IV, compared with Groups I and III. The plasma total antioxidant status (TAS) as well as the MDA and the L: -ascorbic acid level in the erythrocytes (RBCs) remained unchanged in all the groups, whereas the plasma L: -ascorbic acid concentration decreased markedly in Group II, compared with Group III. The activities of Cu,Zn-superoxide dismutase (Cu,Zn-SOD), catalase (CAT), cellular glutathione peroxidase (cGSH-Px), and glutathione reductase (GR) in RBCs remained unaltered in all the treated rats. However, the activity of glutathione S-transferase (GST) and the content of reduced glutathione (GSH) in RBCs decreased and increased, respectively, in Groups II, III, and IV, compared with Group I. A vanadium-chromium interaction which affected the GST activity was also found. To summarize, SMV and CC administered separately or in combination in drinking water for 12 weeks did not alter either lipid peroxidation (LPO) or the activities of Cu,Zn-SOD, CAT, cGSH-Px, and GR, which allows a conclusion that both metals in the doses ingested did not reveal their pro-oxidant potential on RBCs.

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Section: Discussionmentioning

confidence: 79%

Antioxidant enzyme activity and lipid peroxidation in the blood of rats co-treated with vanadium (V+5) and chromium (Cr+3)

et al. 2010

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“…The changes in the activities of a few key enzymes of glycolysis, gluconeogenesis and lipogenic pathways in tissues of experimental diabetic animals together with antidiabetic treatments with insulin, vanadate and Trigonella have been shown (table 2). Reversal with the antidiabetic compounds administered, namely, vanadium, Trigonella, manganese and insulin, showed that most parameters, including blood glucose levels and enzyme changes, reversed to the control levels (Gupta et al 1999;Raju et al 2001;Mohammad et al 2004;Yadav et al 2004;Preet et al 2006).…”

Section: Physiological and Biochemcial Changesmentioning

confidence: 95%

“…Figure 1B shows glucose overutilization and induced potential damage to the tissues (Sochor et al 1985). Mohammad et al (2004) and Siddiqui et al (2006) showed changes in general parameters after the vanadate and Trigonella treatments in diabetic animals. Body weights were significantly reduced in the diabetic groups: vanadate treatment could not improve the weight loss when compared with the controls, whereas insulin, Trigonella and Trigonella and vanadate in combination resulted in significant increase in body weights as compared with the diabetic rats.…”

Section: Metabolic Pathways Affected: Regulation Of Blood Glucosementioning

confidence: 95%

“…Diabetes is associated with altered levels of these enzymes that results in increased oxidative stress. The changes in these enzymes are given in table 2, together with the levels of MDA, a lipid peroxidative product formed in the tissues due to peroxidation of lipids during oxidative stress (Genet et al 2002;Mohammad et al 2004;Siddiqui et al 2005). Advanced glycation end products (AGEs) are formed through oxidative reactions and cause irreversible chemical modifications of protein (Wieland 1983;Mullarky et al 1990).…”

Section: Physiological and Biochemcial Changesmentioning

confidence: 98%

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Metabolic and molecular action of Trigonella foenum-graecum (fenugreek) and trace metals in experimental diabetic tissues

et al. 2011

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Diabetes mellitus is a heterogeneous metabolic disorder characterized by hyperglycaemia resulting in defective insulin secretion, resistance to insulin action or both. The use of biguanides, sulphonylurea and other drugs are valuable in the treatment of diabetes mellitus; their use, however, is restricted by their limited action, pharmacokinetic properties, secondary failure rates and side effects. Trigonella foenum-graecum, commonly known as fenugreek, is a plant that has been extensively used as a source of antidiabetic compounds from its seeds and leaf extracts. Preliminary human trials and animal experiments suggest possible hypoglycaemic and antihyperlipedemic properties of fenugreek seed powder taken orally. Our results show that the action of fenugreek in lowering blood glucose levels is almost comparable to the effect of insulin. Combination with trace metal showed that vanadium had additive effects and manganese had additive effects with insulin on in vitro system in control and diabetic animals of young and old ages using adipose tissue. The Trigonella and vanadium effects were studied in a number of tissues including liver, kidney, brain peripheral nerve, heart, red blood cells and skeletal muscle. Addition of Trigonella to vanadium significantly removed the toxicity of vanadium when used to reduce blood glucose levels. Administration of the various combinations of the antidiabetic compounds to diabetic animals was found to reverse most of the diabetic effects studied at physiological, biochemical, histochemical and molecular levels. Results of the key enzymes of metabolic pathways have been summarized together with glucose transporter, Glut-4 and insulin levels. Our findings illustrate and elucidate the antidiabetic/insulin mimetic effects of Trigonella, manganese and vanadium.

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“…The activity of ROS scavenger compounds like glutathione, catalase and superoxide dismutase may be affected by diabetes (Wohaieb and Godin 1987;Genet et al 2002). Decrease in ROS scavenging compounds may be due to the direct affect of diabetes and aging on scavenger production or activity, like enhanced protein glycation may be responsible for the reduced enzymatic anti-oxidant activity of superoxide dismutase Mohamad et al 2004). Alteration of glutathione levels may be related to an increased polyol pathway (Preet et al 2005) activity as this leads to a depletion of NADPH which is necessary for the enzymatic reduction of oxidized glutathione (Fig.…”

Section: Diabetic Neuropathy-peripheral and Central Nervous Systemmentioning

confidence: 97%

A metabolic and functional overview of brain aging linked to neurological disorders

et al. 2009

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Close correlations have recently been shown among the late onset complications encountered in diabetes and aging linked to neurobiological disorders. Aging in females and males is considered as the end of natural protection against age related diseases like osteoporosis, coronary heart disease, diabetes, Alzheimer's disease and Parkinson's disease, dementia, cognitive dysfunction and hypernatremia. Beside the sex hormones other hormonal changes are also known to occur during aging and many common problems encountered in the aging process can be related to neuroendocrine phenomena. Diabetes mellitus is associated with moderate cognitive deficits and neurophysiologic and structural changes in the brain, a condition that may be referred to as diabetes encephalopathy; diabetes increases the risk of dementia especially in the elderly. The current view is that the diabetic brain features many symptoms that are best described as accelerated brain aging. This review presents and compares biochemical, physiological, electrophysiological, molecular, and pathological data from neuronal tissue of aging and hormone treated control and diabetic animals to arrive at the similarities among the two naturally occuring physiological conditions. Animal models can make a substantial contribution to understanding of the pathogenesis, which share many features with mechanism underlying brain aging. By studying the pathogenesis, targets for pharmacology can be identified, finally leading to delay or prevention of these complications. Antiaging strategies using hormone therapy, chemical and herbal compounds were carried out for reversal of aging effects. Neuronal markers have been presented in this review and similarities in changes were seen among the aging, diabetes and hormone treated (estrogen, DHEA and insulin) brains from these animals. A close correlation was observed in parameters like oxidative stress, enzyme changes, and pathological changes like lipofuscin accumulation in aging and diabetic brain.

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Lower doses of vanadate in combination with trigonella restore altered carbohydrate metabolism and antioxidant status in alloxan-diabetic rats

Cited by 48 publications

References 40 publications

Antioxidant enzyme activity and lipid peroxidation in the blood of rats co-treated with vanadium (V+5) and chromium (Cr+3)

Antioxidant enzyme activity and lipid peroxidation in the blood of rats co-treated with vanadium (V+5) and chromium (Cr+3)

Metabolic and molecular action of Trigonella foenum-graecum (fenugreek) and trace metals in experimental diabetic tissues

A metabolic and functional overview of brain aging linked to neurological disorders

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