Lower Dose of rhBMP-2 Achieves Spine Fusion When Combined With an Osteoconductive Bulking Agent in Non-human Primates

Barnes, Brian R.; Boden, Scott D.; Louis-Ugbo, John; Tomak, Patrick; Park, Jin-Soo; Park, Moon Soo; Minamide, Akihito

doi:10.1097/01.brs.0000162623.48058.8c

Cited by 93 publications

(52 citation statements)

References 28 publications

(27 reference statements)

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“…9,43 Pre-clinical studies have also suggested that because of its favorable binding properties to osteoinductive growth factors such as rhBMP-2, the dose required to achieve successful bony fusion is effectively reduced. 44,45 To this end, recent studies have demonstrated that a 10-15% hydroxyapatite composition in a ceramic carrier is ideal for stem cell adhesion and osteoblastic differentiation. 46,47 In comparison, Grafton 1 is a DBM processed from human tissue into bone fibers combined with glycerol (79-85% content) to achieve a putty-like consistency.…”

Section: Discussionmentioning

confidence: 99%

Nanocomposite therapy as a more efficacious and less inflammatory alternative to bone morphogenetic protein‐2 in a rodent arthrodesis model

Hsu¹,

Polavarapu²,

Riaz³

et al. 2011

Journal Orthopaedic Research

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The use of recombinant human bone morphogenetic protein-2 (rhBMP-2) in spine fusion has led to concerns regarding a potential accompanying inflammatory response. This study evaluates a combination therapy (TrioMatrix 1 ; Pioneer Surgical, Inc., Marquette, MI) comprised of a demineralized bone matrix (DBM), hydroxyapatite, and a nanofiber-based collagen scaffold in a rodent spine fusion model. Thirty-six athymic rats that underwent a posterolateral intertransverse spinal fusion were randomly assigned to 1 of 5 treatment groups: absorbable collagen sponge alone (ACS, negative control), 10 mg rhBMP-2 on ACS (positive control), TrioMatrix 1 , Grafton 1 (Osteotech, Inc., Eatontown, NJ), and DBX 1 (Synthes, Inc., West Chester, PA). Both TrioMatrix 1 and rhBMP-2-treated animals demonstrated 100% fusion rates as graded by manual palpation scores 8 weeks after implantation. This rate was significantly greater than those of the ACS, Grafton 1 , and DBX 1 groups. Notably, the use of TrioMatrix 1 as evaluated by microCT quantification led to a greater fusion mass volume when compared to all other groups, including the rhBMP-2 group. T2-weighted axial MRI images of the fusion bed demonstrated a significant host response associated with a large fluid collection with the use of rhBMP-2; this response was significantly reduced with the use of TrioMatrix 1 . Our results therefore demonstrate that a nanocomposite therapy represents a promising, cost-effective bone graft substitute that could be useful in spine fusions where BMP-2 is contraindicated.

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Section: Discussionmentioning

confidence: 99%

Nanocomposite therapy as a more efficacious and less inflammatory alternative to bone morphogenetic protein‐2 in a rodent arthrodesis model

Hsu¹,

Polavarapu²,

Riaz³

et al. 2011

Journal Orthopaedic Research

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“…26,27 In addition, biomechanical differences have been largely stated and reports concerning differential biological responses -e.g. baboons seem to be hypersensitive to the administration of bone morphogenic proteins 28 contribute to the fact that NHPs do not attain a valid consensus as providing a first choice model, compared to other large animals. 7 As stated, a wide range of animal models is available not only for fundamental, feasibility and bioactivity bonerelated biomedical research, but also for preclinical testing.…”

Section: Selection Of Animal Species -The Relevance Of Rodentsmentioning

confidence: 99%

Rodent models in bone-related research: the relevance of calvarial defects in the assessment of bone regeneration strategies

2011

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In vivo research with animal models has been a preferred experimental system in bone-related biomedical research since, by approximation, it allows relevant data gathering regarding physiological and pathological conditions that could be of use to establish more effective clinical interventions. Animal models, and more specifically rodent models, have been extensively used and have contributed greatly to the development and establishment of a wide range of translational approaches aiming to regenerate the bone tissue. In this regard, the calvarial defect model has found great application in basic and applied research, nonetheless the controversial rationalization for the use of critical size defects -defects that are unable to report spontaneous healing -or subcritical size defects in the proposed applications. Accordingly, this work aims to review the advantages and limitations of the use of rodent models in biomedical bone-related research, emphasizing the problematic issues of the use of calvarial critical and subcritical size defects. Additionally, surgical protocols for the establishment of both defects in rat calvarial bone, as well as the description and exemplification of the most frequently used techniques to access the bone tissue repair, are portrayed.

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“…However, non-unions are seen in the patient material. In the event of forming stable bridging bone in a bone transplant, different bone morphogenetic proteins are active in different time periods in a cascade reaction and perhaps other preparations (Barnes et al 2005, Vaccaro et al 2005a and other bone formation promoters (Boden et al 2002) can bring further improvement.…”

Section: Lumbar Segmental Mobility Adjacent To Fusionmentioning

confidence: 99%

Radiostereometry in lumbar spine research

2006

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Lower Dose of rhBMP-2 Achieves Spine Fusion When Combined With an Osteoconductive Bulking Agent in Non-human Primates

Cited by 93 publications

References 28 publications

Nanocomposite therapy as a more efficacious and less inflammatory alternative to bone morphogenetic protein‐2 in a rodent arthrodesis model

Nanocomposite therapy as a more efficacious and less inflammatory alternative to bone morphogenetic protein‐2 in a rodent arthrodesis model

Rodent models in bone-related research: the relevance of calvarial defects in the assessment of bone regeneration strategies

Radiostereometry in lumbar spine research

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