Lower CD28+ T cell proportions were associated with CMV-seropositivity in patients with Hashimoto’s thyroiditis

Prelog, Martina; Schönlaub, Jörn; Würzner, Reinhard; Koppelstaetter, Christian; Almanzar, Giovanni; Brunner, Andrea; Gasser, Martin; Prommegger, Rupert; Häusler, Gabriele; Kapelari, Klaus; Högler, Wolfgang

doi:10.1186/1472-6823-13-34

Cited by 9 publications

(9 citation statements)

References 35 publications

(55 reference statements)

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“…CMV status was associated with the percentage of the CD8 + CD28 − T cells which was increased in CMV positive early RA patients in comparison to CMV negative early RA patients. CD8 + CD28 − T cells have been found to be significantly increased in CMV positive compared to CMV negative in other autoimmune diseases (25). Our findings that percentage of the CD8 + CD28 − T cells was increased in CMV positive early RA patients in comparison to CMV negative early RA patients was in keeping with this.…”

Section: Discussionsupporting

confidence: 89%

CD28− Cells Are Increased in Early Rheumatoid Arthritis and Are Linked With Cytomegalovirus Status

et al. 2020

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Objective: CD3 + CD8 + CD28 − cells are higher in Rheumatoid Arthritis (RA). The aim of this study was to assess CD3 + CD8 + CD28 − cells in patients with early RA and assess the effects of cytomegalovirus (CMV) seropositivity. Method: In this prospective observation study, 50 RA patients were recruited from Cardiff University Hospital of Wales (UHW) rheumatology outpatient, 25 patients with early disease (disease duration 0-6 months) and 25 patients with established disease (>2 years). These were compared with 25 healthy controls. Clinical and serological markers of inflammation were noted, and peripheral blood mononuclear cells were analyzed using flow cytometry. Results: The percentage of the CD8 + CD28 − T cells was increased in RA patients and was associated with disease duration. The percentage of CD8 + CD28 − T cells was increased in CMV positive early and established RA grouped and early RA patients in comparison to CMV negative patients (p < 0.05). There is a weak but statistically significant correlation between the percentage of CD3 + CD8 + CD28 − cells and CRP in CMV positive RA patients (r = 0.227, p < 0.05). Conclusion: The percentage of CD8 + CD28 − T cells is higher in RA patients and correlates with disease duration, highlighting a potential role early in the disease process. These cells were also higher in CMV positive early RA patients which may suggest a role of CMV in disease development.

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Section: Discussionsupporting

confidence: 89%

CD28− Cells Are Increased in Early Rheumatoid Arthritis and Are Linked With Cytomegalovirus Status

et al. 2020

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“…In this study, nominally reduced levels of CD28 mRNA expression and the peripheral loss of CD45RAB mRNA were associated with HT development. Other analyses support these results: a parallel loss of CD28 expression and the peripheral reduction of CD45RA+ T-cells have recently been described in HT [41]. Several groups have reported an expansion of CD4+ T-cells lacking CD28 in chronic inflammatory disorders [44][45][46], as well as in some normal-aged subjects [47].…”

Section: Discussionsupporting

confidence: 57%

“…A critical phenotypic and genetic change as T-cells becomes late-differentiated and progress to senescence is the loss of gene and surface expression of CD28 [43]. CD28 surface expression in HT is not steady and is influenced by several mechanisms [41]. In this study, nominally reduced levels of CD28 mRNA expression and the peripheral loss of CD45RAB mRNA were associated with HT development.…”

Section: Discussionmentioning

confidence: 52%

“…In the present study, CD45 composition was apparently affected by age, thyroid hormones, and case-control status, suggesting a shift from long/ CD45RA+ isoforms to short/CD45R0 transcripts in an inverse, age-and T3-dependent manner. Higher proportions of both CD4+ and CD8+ T cells expressing CD45R0 have previously been detected in HT patients [41]; moreover, these cells have been functionally char-acterised as memory/effector T-cells and high producers of proinflammatory cytokines TNFα and IFNα [42]. Here, older age and lower T3 levels have been associated with the accumulation of CD45R0 isoform in HT patients, but not controls, a pattern suggestive of accelerated T-cell ageing in elderly, hypothyroid HT subjects.…”

Section: Discussionmentioning

confidence: 55%

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Zmiana ekspresji mRNA dla CTLA-4, CD28, VDR i CD45 w limfocytach T u osób z chorobą Hashimoto — badanie pilotowe

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Karner

et al. 2017

Endokrynologia Polska

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“…Impaired central tolerance in autoimmune endocrine disorders is a relatively new concept. However, up to this date, only a few studies have investigated circulating TRECs in patients with T1D [15], Hashimoto thyroiditis [36], and Graves disease [16, 37], and there are no data regarding KREC measurement in any autoimmune endocrine disorders. Hofer et al [15] observed increased circulating TRECs in 38 patients with T1D with mean age of 10 to 15 years.…”

Section: Discussionmentioning

confidence: 99%

Central Immune Tolerance of T and B Cells in Patients With Idiopathic Hypoparathyroidism, T1D, and Autoimmune Thyroiditis

Mahtab

Kar

Saha

et al. 2019

Journal of the Endocrine Society

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Context Pathogenesis of idiopathic hypoparathyroidism (IH) is under investigation. Abnormalities in central immune tolerance have yet not been investigated in this condition. T-cell receptor excision circles (TRECs) and kappa-deleting recombination excision circles (KRECs), formed during receptor gene rearrangements, are tools to assess central T- and B-cell output. Objective We assessed the number of circulating TRECs and KRECs in patients with IH, autoimmune type 1 diabetes (T1D), and autoimmune thyroiditis (ATs) and healthy controls (HCs). Design Comparative case-control at tertiary care center. Subjects and Methods Absolute and relative TRECs and KRECs were measured in DNA extracted from whole blood of patients with IH (n = 181, 22 of whom were reassessed after a decade of follow-up) and T1D (n = 133), AT (n = 53), and HC (n = 135) using a quantitative real-time PCR/TaqMan ® probe technique. Results Absolute and relative means of TRECs and KRECs in IH were comparable to HCs, and no differences were found between IH with and without calcium-sensing receptor antibodies or class I HLA-A*26:01 association. TRECs and KRECs did not change after a decade of follow-up. T1D had significantly higher absolute TRECs than IH, AT, and HCs, whereas AT patients showed lower TRECs and the highest KRECs; these levels showed no noteworthy correlation with thyroid dysfunctions. Conclusion Patients with IH showed TRECs and KRECs comparable to HCs, indicating an intact mechanism of T- and B-cell central immune tolerance. Interestingly, absolute TRECs were significantly higher in T1D than HCs, suggesting impaired central immune tolerance in T1D.

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Lower CD28+ T cell proportions were associated with CMV-seropositivity in patients with Hashimoto’s thyroiditis

Cited by 9 publications

References 35 publications

CD28− Cells Are Increased in Early Rheumatoid Arthritis and Are Linked With Cytomegalovirus Status

CD28− Cells Are Increased in Early Rheumatoid Arthritis and Are Linked With Cytomegalovirus Status

Zmiana ekspresji mRNA dla CTLA-4, CD28, VDR i CD45 w limfocytach T u osób z chorobą Hashimoto — badanie pilotowe

Central Immune Tolerance of T and B Cells in Patients With Idiopathic Hypoparathyroidism, T1D, and Autoimmune Thyroiditis

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