Low warfarin resistance frequency in Norway rats in two cities in China after 30 years of usage of anticoagulant rodenticides

Ma, Xiaohui; Wang, Dawei; Li, Ning; Liu, Lan; Tian, Lin; Luo, Chan; Cong, Lin; Feng, Zheng; Liu, Xiaohui; Song, Ying

doi:10.1002/ps.5040

Cited by 12 publications

(16 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The same consequences could therefore be suspected: the selection of individuals carrying Vkorc1 mutations conferring them a strong resistance to these molecules. Indeed, in brown rats, more than 15 different mutations have been described worldwide (Rost et al 2004;Pelz et al 2005 ;Grandemange et al 2010;Ma et al, 2018, Diaz et al, 2021, including some associated with severe resistance (Y139F, Y139C and L120Q) that can reach very high allelic frequency up to 100% locally. In mice, the same observations were made (Goulois et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…This intense use has resulted in the selection of resistant rodents. This resistance was first identified in England in 1960 (Boyle, 1960;Dodsworth, 1961) and then described worldwide (Jackson and Kaukeinen, 1972;Siddiq and Blaine, 1982;Pelz, 2007;Grandemange et al, 2010;Goulois et al, 2017;Ma et al, 2018;Diaz et al, 2021). Different mechanisms have been demonstrated or suggested.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Seasonal diet-based resistance to anticoagulant rodenticides in the fossorial water vole (Arvicola amphibius)

Khalil

Barbier²,

Fafournoux³

et al. 2021

Environmental Research

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Seasonal diet-based resistance to anticoagulant rodenticides in the fossorial water vole (Arvicola amphibius)

Khalil

Barbier²,

Fafournoux³

et al. 2021

Environmental Research

View full text Add to dashboard Cite

“…The exceptions are data from studies dealing with rodent eradications on islands where the aerial application of the low-dose brodifacoum baits are commonly used 5,34,38 . Thus, our results could open a discussion on the topic of low-dose anticoagulant baits in the scientific literature, where other related topics are currently highlighted, e.g., resistance and secondary intoxications 2,7,39–41 .…”

Section: Discussionmentioning

confidence: 82%

Efficacy of rodenticide baits with decreased concentrations of brodifacoum: Validation of the impact of the new EU anticoagulant regulation

Fraňková

Stejskal

Aulický

2019

Sci Rep

View full text Add to dashboard Cite

Anticoagulants are the most frequently used rodenticides at the global scale. Because of their persistency, bioaccumulation and potential for secondary intoxication, they have faced increasing legislative regulations. Recently, the European Union Regulation (EU) 2016/1179 resulted in the production and application of rodenticides with nearly half dose (<30 ppm) of anticoagulants. However, published data on the biological efficacy of rodenticides with decreased doses are scarce in the EU. Therefore, this work compared the efficacy of the original high-dose (50 ppm) and new low-dose (25 ppm) brodifacoum-based baits in the offspring of wild-caught house mice (Mus musculus L.). In the no-choice laboratory feeding tests, 100% animals died in all treated groups and 0% died in the control groups. The achieved time to death did not differ between the original and low-dose baits across both types of feeding trials/regimes. The low-dose baits (25 ppm) were consequently tested under field conditions in two populations showing 95.7% and 99.8% efficacy. The obtained results highlighted the good efficacy of the new baits based on low-dose brodifacoum in non-resistant mouse populations. However, further validation is required regarding the remaining anticoagulant compounds and resistant rodent populations.

show abstract

“…As such, the exon 3 SNP pro le of rats in Singapore was unexpected, since rodenticides have been used for the past 20 years. Likewise for China, anticoagulant rodenticides have been widely used for over 30 years and yet the frequency of resistance has been reported to be low [44] .…”

Section: Discussionmentioning

confidence: 99%

Single Nucleotide Polymorphisms (SNPs) Within Vkorc1 in Rodent Populations in a Tropical City-state: Implications for Anticoagulant Rodenticide Use for Rodent Control

Chua¹,

Humaidi²,

Ng³

et al. 2021

Preprint

View full text Add to dashboard Cite

Anticoagulant rodenticides are commonly used in rodent control because they are economical and have great deployment versatility. However, rodents with Single Nucleotide Polymorphism (SNP) mutations within the Vkorc1 gene are resistant to the effects of anticoagulant rodenticide use and this influences the effectiveness of control strategies that rely on such rodenticides. This study examined the prevalence of rat SNP mutations in Singapore to inform the effectiveness of anticoagulant rodenticide use. A total of 130 rat tail samples, comprising 83 Rattus norvegicus (63.8%) and 47 Rattus rattus spp. (36.2%) were conveniently sampled from November 2016 to December 2019 from urban settings and sequenced at exon 3 of Vkorc1. Sequencing analysis revealed 4 synonymous and 1 non-synonymous mutations in Rattus rattus spp. samples. A novel synonymous mutation of L108L was identified and not previously reported in other studies. Non-synonymous SNPs were not detected in the notable codons of 120, 128 and 139 in Norway rats, where these regions are internationally recognised to be associated with resistance from prior studies. Our findings suggest that the prevalence of anticoagulant rodenticide resistance in Singapore is low. Continued monitoring of rodenticide resistance is important for informing rodent control strategies aimed at reducing rodent-borne disease transmission.

show abstract

Low warfarin resistance frequency in Norway rats in two cities in China after 30 years of usage of anticoagulant rodenticides

Cited by 12 publications

References 37 publications

Seasonal diet-based resistance to anticoagulant rodenticides in the fossorial water vole (Arvicola amphibius)

Seasonal diet-based resistance to anticoagulant rodenticides in the fossorial water vole (Arvicola amphibius)

Efficacy of rodenticide baits with decreased concentrations of brodifacoum: Validation of the impact of the new EU anticoagulant regulation

Single Nucleotide Polymorphisms (SNPs) Within Vkorc1 in Rodent Populations in a Tropical City-state: Implications for Anticoagulant Rodenticide Use for Rodent Control

Contact Info

Product

Resources

About