Low-visibility light-intensity laser-triggered release of entrapped calcein from 1,2-bis (tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine liposomes is mediated through a type I photoactivation pathway

Puri, Anu; Viard, Mathias; Viard,; Sine, Jessica; Vu, Mylinh; Vu, Vu; Blumenthal, D.J.; Tata, Darrell B.

doi:10.2147/ijn.s44993

Cited by 5 publications

(1 citation statement)

References 41 publications

(71 reference statements)

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“…Among a variety of phospholipids, DPPC is the strongest surfactant molecule, with higher compaction capacity and very low adsorption kinetics. − Pure DPPC liposomes exhibit content leakage at a lower rate and concentration. ,, DPPC/DC 8,9 PC liposomes were prepared previously for triggered release of calcein . Interestingly, DC 8,9 PC possesses diacetylenic groups in their aliphatic chain that are used to produce intermolecular cross-linking under UV irradiation aimed at achieving greater molecular cohesion and stability. − Exposing DPPC/DC 8,9 PC liposomes to 254 nm wavelength laser light for 0–45 min induces phase boundary defects in the lipid bilayer, resulting in content release that is highly dependent on the DPPC/DC 8,9 PC molar ratio …”

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Physicochemical Analysis of DPPC and Photopolymerizable Liposomal Binary Mixture for Spatiotemporal Drug Release

Kenaan

Jin

et al. 2018

Anal. Chem.

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The development of a spatiotemporal drug delivery system with a long release profile, high loading efficiency, and robust therapeutic effects is still a challenge. Liposomal nanocarriers have secured a fortified position in the biomedical field over decades. Herein, liposomal binary mixtures of 1,2-dipalmitoyl- sn-glycero-3-phosphocholine (DPPC) and photopolymerizable 1,2-bis(10,12-tricosadiynoyl)- sn-glycero-3-phosphocholine (DCPC) phospholipids were prepared for drug delivery applications. The diacetylenic groups of DCPC produce intermolecular cross-linking following UV irradiation. Exposure of the liposomal mixture to 254 nm radiation induces a pore within the lipid bilayer, expediting the release of its entrapped 5,6-carboxyfluorescein dye. The dosage and rate of the released content are highly dependent on the number and size of the induced pore. Photochemical cross-linking studies at different exposure times were reported through the analysis of UV-visible spectrophotometry, nano differential scanning calorimetry, Fourier transform infrared spectroscopy, and Raman spectroscopy. The optimal irradiation time was established after 8 min of exposure, inducing lipid cross-linking with minimal oxidative degradation, which plays an essential role in the pathogenesis of numerous diseases due to the formation of primary and secondary oxidation products, accordingly reducing the encapsulated drug therapeutic level.

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confidence: 99%

Physicochemical Analysis of DPPC and Photopolymerizable Liposomal Binary Mixture for Spatiotemporal Drug Release

Kenaan

Jin

et al. 2018

Anal. Chem.

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Effect of Laser Irradiation on Reversibility and Drug Release of Light-Activatable Drug-Encapsulated Liposomes

et al. 2020

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Although several studies have demonstrated repetitive drug release using light-activatable liposomes, inconsistent drug release at each activation limits widespread usage. Here, we report reversible plasmonic material-coated encapsulated liposomes for proportional controlled delivery of methotrexate (MTX), which is a common drug for cancer and autoimmune diseases, using repetitive laser irradiation. Our results suggest a proportional increase in total drug release after repetitive laser irradiation. We hypothesize that the drug is released via "melted" lipid bilayers when the plasmonic materials on the liposome surface are heated by laser irradiation followed by reversible formation of the liposome. To evaluate our hypothesis, the number density of liposomes after laser irradiation was measured using single-particle (liposome) collision experiments at an ultramicroelectrode. Collisional frequency data suggest that the number density of liposomes remains unaltered even after 60 s of laser irradiation at 1.1 and 1.8 W, indicating that the liposome structure is reversible. The results were further compared with gold nanorod-coated nanodroplets where drug is released via irreversible phase transition. In contrast to what was observed with the liposome particles, the number density of the nanodroplets decreased with increasing laser irradiation duration. The structure reversibility of our liposome particles may be responsible for repetitive drug release with laser heating. We also studied the temperature rise in the lipid bilayer by incorporating polymerized 10,12pentacosadiynoic acid (PCDA) in the lipid composition. The red shift in the UV−vis spectrum due to the structural change in PCDA lipids after laser irradiation indicates a rise in temperature above 75 °C, which is also above the chain-melting temperature of the main lipid used in the liposomes. All these results indicate that drug is released from the light-activatable liposomes due to reversible nanostructural alteration in the lipid bilayer by plasmonic resonance heating. The liposomes have potential to be a drug carrier for dose-controlled repetitive drug delivery.

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Laser-controlled Interaction of Cytocrome c with Lipids May Not Disrupt Apoptotic Pathway

Sichevska

Герасимова

Berest

et al. 2019

2019 IEEE 8th International Conference on Advanced Optoelectronics and Lasers (CAOL)

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Low-visibility light-intensity laser-triggered release of entrapped calcein from 1,2-bis (tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine liposomes is mediated through a type I photoactivation pathway

Cited by 5 publications

References 41 publications

Physicochemical Analysis of DPPC and Photopolymerizable Liposomal Binary Mixture for Spatiotemporal Drug Release

Physicochemical Analysis of DPPC and Photopolymerizable Liposomal Binary Mixture for Spatiotemporal Drug Release

Effect of Laser Irradiation on Reversibility and Drug Release of Light-Activatable Drug-Encapsulated Liposomes

Laser-controlled Interaction of Cytocrome c with Lipids May Not Disrupt Apoptotic Pathway

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