Low total IgM values and high cytomegalovirus loads in the blood of newborns with symptomatic congenital cytomegalovirus infection

Kobayashi, Yôko; Morioka, Ichiro; Koda, Tsubasa; Nakamachi, Yuji; Okazaki, Yoko; Noguchi, Yoriko; Ogi, Miki; Chikahira, Masatsugu; Tanimura, Kenji; Ebina, Yasuhiko; Funakoshi, Toru; Ohashi, Masanao; Iijima, Kazumoto; Inoue, Naoki; Yamada, Hideto

doi:10.1515/jpm-2014-0071

Cited by 15 publications

(17 citation statements)

References 26 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…a positive result of real-time quantitative PCR for CMV DNA in the urine as a confirmed diagnosis, as previously established [16]. Diagnoses were made within 3 weeks of birth in all cases [15].…”

Section: Methodsmentioning

confidence: 79%

“…Congenital CMV infection was definitively diagnosed based on the following methods: A positive result of a filter paper-based screening to detect CMV DNA in urine, a method developed previously by our team [15] or a presence of clinical symptoms and findings of congenital CMV infection;a positive result of real-time quantitative PCR for CMV DNA in the urine as a confirmed diagnosis, as previously established [16]. Diagnoses were made within 3 weeks of birth in all cases [15].…”

Section: Methodsmentioning

confidence: 99%

“…Following a confirmed diagnosis, symptoms were determined based on the findings of the physical exams, hematological testing, chest X-rays, abdominal ultrasound, fundoscopy, ABR testing, and brain imaging (ultrasound, computed tomography, and magnetic resonance imaging). Congenital CMV infection was considered “symptomatic” if one or more of the following symptoms were present: microcephaly, hepatosplenomegaly/hepatitis, thrombocytopenia, brain imaging abnormalities, eye complications such as retinal choroiditis, and abnormal ABR [16,17,18]. Microcephaly was defined as a head circumference ≥-1.5 standard deviation, compared to the average for Japanese neonates at the corresponding gestational age [19].…”

Section: Methodsmentioning

confidence: 99%

“…Using a previously reported method [16], DNA was extracted from the peripheral blood and urine using QIAamp DNA Mini kits (Qiagen Corp., Tokyo, Japan). CMV-DNA copy number (CN) was measured using real-time quantitative PCR.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Efficacy of Valganciclovir Treatment Depends on the Severity of Hearing Dysfunction in Symptomatic Infants with Congenital Cytomegalovirus Infection

Ohyama

Morioka

Fukushima

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

Although earlier studies have shown that antiviral treatment regimens using valganciclovir (VGCV) improved hearing function in some infants with congenital cytomegalovirus (CMV) infection; its efficacy on the severity of hearing dysfunction is unclear. We conducted a prospective study among 26 infants with congenital CMV infections from 2009 to 2018. Oral VGCV (32 mg/kg/day) was administered for 6 weeks (November 2009 to June 2015; n = 20) or 6 months (July 2015 to March 2018, n = 6). Hearing function was evaluated by measuring the auditory brainstem response before VGCV treatment and at 6 months. Hearing dysfunction, defined as a V-wave threshold >40 dB, was categorized into: most severe, ≥91 dB; severe, 61–90 dB; and moderate, 41–60 dB. Hearing improvement was defined as a decrease of ≥20 dB from the pretreatment V-wave threshold. Of 52 ears in 26 infants with congenital CMV infection, 29 (56%) had hearing dysfunction, and of 29 ears, 16 (55%) improved after VGCV treatment. Although, 16 (84%) of 19 ears with moderate or severe hearing dysfunction improved after treatment (p < 0.001), 10 ears with the most severe form did not. In conclusion, VGCV treatment is effective in improving moderate and severe hearing dysfunction in infants with congenital CMV infection.

show abstract

Section: Methodsmentioning

confidence: 79%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Efficacy of Valganciclovir Treatment Depends on the Severity of Hearing Dysfunction in Symptomatic Infants with Congenital Cytomegalovirus Infection

Ohyama

Morioka

Fukushima

et al. 2019

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…Once the frozen‐thawed breast milk–feeding period was over, infants could start fresh breast milk feeding. For infants with negative results in the initial CMV screening, urine CMV DNA detection by real‐time quantitative polymerase chain reaction (PCR) was performed using a liquid urine sample obtained at least 3 weeks after birth and before starting fresh breast milk feeding . A positive result indicated acquired CMV infection (Fig.…”

Section: Methodsmentioning

confidence: 99%

Sequence analyses of variable cytomegalovirus genes for distinction between breast milk– and transfusion‐transmitted infections in very‐low‐birth‐weight infants

et al. 2018

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Cytomegalovirus (CMV) transmission to very-low-birth-weight infants (VLBWIs) sometimes induces serious clinical symptoms. Although breast milk is considered a major source of transmission, transfusion-transmitted CMV (TT-CMV) infection is often suspected when CMV disease develops after transfusion. Thus, it is clinically important to distinguish between transfusion-transmitted and breast milktransmitted CMV infections. STUDY DESIGN AND METHODS: Study A: The incidence of acquired CMV transmission was prospectively investigated in 65 VLBWIs. Study B: To determine the transmission routes in 18 TT-CMVsuspected VLBWIs who had been reported in our hemovigilance system, we performed polymerase chain reaction for CMV DNA in fed breast milk and/or repository blood samples related to transfused leukoreduced blood products. Furthermore, we evaluated the identity of CMV strains in patients' urine/blood samples and fed breast milk by sequence analyses of variable CMV genes UL139 and UL146.RESULTS: Study A: Acquired CMV infection was found in 4 of 65 VLBWIs (6.2%). Study B: CMV DNA was detected in fed breast milk for 12 of 14 TT-CMVsuspected cases, for which breast milk was available. Furthermore, CMV DNA sequence-matching rates between fed breast milk and patients' urine/blood for both UL139 and UL146 genes were 100% or nearly 100% in 11 patients. In contrast, repository blood samples for 11 of 14 patients were CMV DNA negative.CONCLUSION: CMV is principally transmitted through breast milk in VLBWIs. The risk of TT-CMV seems to be extremely low when using leukoreduced blood products. Sequence analyses of the variable CMV genes are useful for evaluating CMV transmission routes. ABBREVIATIONS: BM-CMV = breast milk-transmitted CMV; CMV = cytomegalovirus; JRCS = Japanese Red Cross Society; LOD = limit of detection; PCR = polymerase chain reaction; TT-CMV = transfusiontransmitted CMV; VLBWI = very-low-birth-weight infantFrom the * The number of tested repository blood samples is the same as the number of transfused blood products. † Homology (the repository blood sample vs. patient) is 64.6% for the UL139 gene and 68.6% for the UL146 gene. 16 ‡ CMV DNA was detected in the testing conducted at the hospital. § Homology (the repository blood sample vs. patient) is 89.3% for the UL139 gene and 78.1% for the UL146 gene. FFP = fresh-frozen plasma; NT = not tested; PC = platelet concentrate; PCR = polymerase chain reaction; RBC = red blood cell. TRANSFUSION Volume 58, December 2018FURUI ET AL.

show abstract

Viral, Protozoan, and Related Intracranial Infections

Vries¹,

Volpe²

2018

Volpe's Neurology of the Newborn

View full text Add to dashboard Cite

Low total IgM values and high cytomegalovirus loads in the blood of newborns with symptomatic congenital cytomegalovirus infection

Abstract: Low total IgM values and high blood CMV loads were associated with the presence of symptoms in newborns with congenital CMV infection.

Cited by 15 publications

References 26 publications

Efficacy of Valganciclovir Treatment Depends on the Severity of Hearing Dysfunction in Symptomatic Infants with Congenital Cytomegalovirus Infection

Efficacy of Valganciclovir Treatment Depends on the Severity of Hearing Dysfunction in Symptomatic Infants with Congenital Cytomegalovirus Infection

Sequence analyses of variable cytomegalovirus genes for distinction between breast milk– and transfusion‐transmitted infections in very‐low‐birth‐weight infants

Viral, Protozoan, and Related Intracranial Infections

Contact Info

Product

Resources

About