Low temperature storage of suspension culture-derived grapevine somatic embryos and regeneration of plants

Subramanian, Jayasankar; Aman, M. Van; Cordts, John; Dhekney, Sadanand A.; Li, Z. T.; Gray, D. J.

doi:10.1079/ivp2005697

Cited by 16 publications

(9 citation statements)

References 17 publications

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“…Resulting plants tended to acclimatize more easily to the soil environments. These results are similar to those reported by Jayasankar et al (2005), where SE of V. vinifera 'Chardonnay' produced by liquid medium culture had significantly smaller cotyledons and exhibited more robust plant development characteristics than SE with larger cotyledons. Based on these results, it appears that cotyledons exert an inhibitory effect on shoot apical meristem development in grape SE; their removal after germination facilitates shoot growth and plant recovery.…”

Section: Germination Of Transgenic Se and Plant Recoverysupporting

confidence: 90%

An improved protocol for Agrobacterium-mediated transformation of grapevine (Vitis vinifera L.)

Dhekney

Dutt

et al. 2008

Plant Cell Tiss Organ Cult

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An improved protocol for efficient Agrobacterium-mediated transformation of grapevine (Vitis sp.) was developed through modification of cocultivation and subsequent washing procedures. It was determined that Agrobacterium-infected somatic embryos (SE) cocultivated on filter paper exhibited less browning and significantly higher transient GFP and GUS expression than those cultured on agarsolidified medium. Furthermore, such SE, when subjected to a prolonged washing period in liquid medium containing cefotaxime and carbenicillin, followed by another wash in similar medium with kanamycin added, exhibited significantly higher rates of stable transformation compared to previouslydescribed procedures. Transgenic plant recovery was increased 3.5-6 Xs by careful excision of leafy cotyledons from SE that had been induced to germinate on MS medium containing 1 lM of BA. Southern blot analysis revealed the low copy number integration of transgenes in transgenic plants recovered using the improved protocol. These improved cocultivation and plant recovery procedures have been demonstrated to facilitate production of large populations of transgenic plants from V. vinifera 'Merlot', 'Shiraz' and 'Thompson Seedless' as well as Vitis hybrid 'Seyval Blanc'.

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Section: Germination Of Transgenic Se and Plant Recoverysupporting

confidence: 90%

An improved protocol for Agrobacterium-mediated transformation of grapevine (Vitis vinifera L.)

Dhekney

Dutt

et al. 2008

Plant Cell Tiss Organ Cult

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“…1H) and successfully established in a greenhouse after acclimatization. Several treatments have been used to improve grapevine SE germination (Gray, 1989;Jayasankar et al, 2005); however, cotyledon excision of germinated SE is a simple method resulting in greater plant recovery .…”

Section: Resultsmentioning

confidence: 99%

Optimizing Initiation and Maintenance of Vitis Embryogenic Cultures

Dhekney¹,

Li²,

Compton³

et al. 2009

horts

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Stamens and pistils from mature grapevines and leaves from in vitro micropropagation cultures were used to optimize parameters influencing somatic embryogenesis in Vitis. Embryogenic competence was dependent on species/variety, explant type and developmental stage, medium composition, and growth regulator concentration. Of varieties evaluated, a greater number produced embryogenic cultures from stamens and pistils (26) compared with leaves (six). Among the different stamen and pistil stages, Stage II and III explants produced the maximum embryogenic response regardless of genotype and medium composition. Of seven culture media tested, the highest embryogenic response was recorded from varieties cultured on MSI (18) and PIV (16) media. Experiments annually repeated over 3 to 10 years demonstrated reproducible results. Highly reliable protocols for somatic embryogenesis were obtained for 29 Vitis species and varieties, including 18 Vitis vinifera varieties, Vitis riparia, Vitis rupestris, Vitis champinii, and eight Vitis hybrids. Embryogenic cultures were maintained on X6 medium for a period of 6 months to 2 years depending on the variety and used in studies involving genetic transformation and transgenic plant regeneration.

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“…Also, in a study on the effects of desiccation and different storage period on the vigor of somatic embryos of carrots, Shao-Xing (1992) observed 67% conversion of dried somatic embryos to plantlets after four months of storage at room temperature. Recently, Jayasankar et al (2005) reported that 90% of dehydrated somatic embryos of grapevine (a temperate species) regenerated into plants after 42 months of storage at 4EC. Also, Corredoira et al (2008) reported that drying in a laminar flow hood for two hours reduced the somatic embryo moisture content to 57-58% and enhanced the potential plant recovery in chestnuts by 37% after two months of cold storage at 4EC.…”

Section: Discussionmentioning

confidence: 99%

“…In carrots, Shao-Xing (1992) observed 67% conversion of dried somatic embryo to plantlets after four months of storage. Similarly, Jayasankar et al (2005) reported that 90% of somatic embryos dehydrated to 17% moisture content in grapevine (a temperate species) regenerated into plants after 42 months of storage at 4EC. To our knowledge, somatic embryogenesis and plant regeneration from low-temperature preserved cassava somatic embryo has not been reported.…”

Section: Introductionmentioning

confidence: 92%

Somatic embryogenesis and genetic uniformity of regenerated cassava plants from low-temperature preserved secondary somatic cotyledons

Opabode

Ajibola

Oyelakin

et al. 2015

bta

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Low plant regeneration and transformation frequencies in cassava have been attributed to recycling of cassava secondary somatic embryos for long periods. This study examined the suitability of low-temperature storage of dehydrated cassava secondary somatic embryos as an alternative short-term conservation approach to recycling. The study included two experiments: in the first one, somatic embryogenesis from cotyledons of dehydrated secondary somatic embryos stored at six temperatures for 4, 8 and 12 months was established; and in the second, plant regeneration via shoot organogenesis using somatic cotyledon explants obtained in the first experiment was examined. The growth, photosynthetic pigment contents and genetic stability of the regenerated plants were assessed. At each storage temperature, the survival of explants, somatic embryogenesis, shoot induction and elongation decreased with increases in storage period. At 4 months after storage (MAS), explant survival, frequency of somatic embryogenesis, shoot induction, elongation and rooting had the best responses at 16EC and 20EC. Similarly, the growth and photosynthetic pigment contents of plants regenerated from somatic embryos preserved at 16EC and 20EC recorded the best results at 4 MAS. However, at 8 and 12 MAS, the best explant survival, somatic embryogenesis, shoot induction and elongation were recorded at 16EC. Eight RAPD primers produced a total of 56 distinct and scorable bands, ranging from 250 bp to 3000 bp in size. An random amplified DNA (RAPD) analysis showed a uniform banding profile among regenerants and to that of the mother plant, indicating no genetic variation among regenerants and between the regenerants and the mother plant. The data presented here suggest that cassava secondary somatic embryos dehydrated to 50% moisture content should be preserved at 16-20EC for 4 months; for 8 and 12 months storage, the embryos should be stored at 16EC. No genetic alteration was detected among the regenerants. Therefore, the method is a suitable means for short-term conservation of cassava secondary somatic embryos.

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Low temperature storage of suspension culture-derived grapevine somatic embryos and regeneration of plants

Cited by 16 publications

References 17 publications

An improved protocol for Agrobacterium-mediated transformation of grapevine (Vitis vinifera L.)

An improved protocol for Agrobacterium-mediated transformation of grapevine (Vitis vinifera L.)

Optimizing Initiation and Maintenance of Vitis Embryogenic Cultures

Somatic embryogenesis and genetic uniformity of regenerated cassava plants from low-temperature preserved secondary somatic cotyledons

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