Low temperature storage of plant tissue cultures

Withers, Lyndsey A.

doi:10.1007/3-540-09936-0_4

Cited by 13 publications

(9 citation statements)

References 156 publications

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“…Damaged cells may become more stressed on shaking and deplasmolysis injury may be greater in liquid culture. Since freshly thawed cells are often 'leaky', important metabolites may be further diluted in the liquid medium [8]. Although hairy roots were routinely grown in liquid culture, postfreeze recovery was examined for both solid and liquid culture conditions ( Table 1).…”

Section: Resultsmentioning

confidence: 99%

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Cryopreservation and post freeze molecular and biosynthetic stability in transformed roots of Beta vulgaris and Nicotiana rustica

Benson

Hamill

1991

Plant Cell Tiss Organ Cult

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Crypopreservation methods were firstly developed for root-tips from hairy root cultures of Beta vulgaris, established after transformation by Agrobacterium rhizogenes. The effects of culture age, pre-growth, cryoprotection, freezing rate and post-freeze culture conditions were determined. The resulting freezing protocol was then used to cryopreserve transformed root cultures of Nicotiana rustica. Both species were viable after freezing (ca. 80%), according to fluorescein diacetate vital staining. However, on average the regeneration of proliferating roots from surviving root-tips was low (< 20%). Growth rates, secondary metabolite production and T-DNA structure of a number of hairy root lines were examined and found to be unchanged after cryopreservation.

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Section: Resultsmentioning

confidence: 99%

“…Three levels of evaluation were used, and data represent recovery as a % of the total number of root-tips frozen. Viability was assessed using fluorescein diacetate vital staining [8]. After 3-5 days the cultures were microscopically examined for signs of early proliferation.…”

Section: Assessment Of Recoverymentioning

confidence: 99%

Cryopreservation and post freeze molecular and biosynthetic stability in transformed roots of Beta vulgaris and Nicotiana rustica

Benson

Hamill

1991

Plant Cell Tiss Organ Cult

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“…Withers (1980) reviewed these substances and techniques in detail. The most commonly used cryoprotectants are various types of glycol (ethylene glycol, propylene glycol, glycerol), DMSO, sugars (most notably trehalose, glucose and sucrose), amino acids, methanol, polymers or colloids, and other specific mixtures.…”

Section: Cryoprotectantsmentioning

confidence: 99%

A hands-on overview of tissue preservation methods for molecular genetic analyses

2010

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“…Reinert and Backs [15] succeeded in regulating and maintaining the totipotency of carrot cells over prolonged periods. Organonenetic ability of plant tissue culture has been maintained by minimal growth medium [16] or by freeze-preservation [6,21]. Few workers, however, used a chilling method for this purpose.…”

Section: Discussionmentioning

confidence: 99%

Effects of non-frozen cold storage on the growth, organogenesis and secondary metabolism of callus cultures

Hiraoka

Kodama

1984

Plant Cell Tiss Organ Cult

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Callus tissues derived from chilling-tolerant herbaceous plant, Atractylodes lancea, Atropa belladonna, Bupleurum falcatum, Dioscorea tokoro, Lithospermum erythrorhizon and Phytolacca americana could be cold-stored at 4°C for three months or more, whereas those from chilling-sensitive herbaceous plants such as Datura innoxia and Perilla frutescens vat. crispa and a deciduous tree, Mallotus ]aponicus, could not survive after cold storage for two to three months. Tobacco callus cultures could be stored at 4°C for two or four months depending on a callus strain. The effect of cold storage on secondary metabolite production varied. Nicotine and betalain production suffered from cold storage of tobacco and Phytolacca americana callus cultures, respectively. However, production of anthocyanin in cultures of Mallotus ]aponicus and Bupleurum falcatum and shikonin derivatives in Lithospermum erythrorhizon callus was affected very little. Root-forming ability was retained for more than one year in cold-stored callus tissues of Bupleurum falcatum, while the control callus tissues maintained at 25°C completely lost the organogenetic ability six months after the first subculture.

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Low temperature storage of plant tissue cultures

Cited by 13 publications

References 156 publications

Cryopreservation and post freeze molecular and biosynthetic stability in transformed roots of Beta vulgaris and Nicotiana rustica

Cryopreservation and post freeze molecular and biosynthetic stability in transformed roots of Beta vulgaris and Nicotiana rustica

A hands-on overview of tissue preservation methods for molecular genetic analyses

Effects of non-frozen cold storage on the growth, organogenesis and secondary metabolism of callus cultures

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