Low-temperature rapid synthesis of nitrogen and phosphorus dual-doped carbon dots for multicolor cellular imaging and hemoglobin probing in human blood

Huang, Shan; Yang, Erli; Liu, Yi; Yao, Jinhuan; Su, Wei

doi:10.1016/j.snb.2018.03.056

Cited by 48 publications

(32 citation statements)

References 33 publications

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“…The limit of detection (LOD) was estimated as 7.8 nM and the limit of quantification (LOQ) as 26.0 nM. The sensorial efficiency toward haemoglobin, as measured by the Stern-Volmer constant, is higher or similar to other reported results [15,[31][32][33][34]37].…”

Section: Detection Of Hemeproteinssupporting

confidence: 80%

“…The last feature proved common for metHgb and Cyt c. An additional redshift (~2 nm) was also observed at the absorption maximum (411 nm) of Cyt c after complexation with C-dots (see details on this point later on). Hyperchromicity [38] and hypochromicity [31,33] effects upon complexation have been noticed before.…”

Section: Understanding the Sensitivity Of C-dots Toward Hemeproteinsmentioning

confidence: 57%

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Finding Value in Wastewaters from the Cork Industry: Carbon Dots Synthesis and Fluorescence for Hemeprotein Detection

et al. 2020

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Valorisation of industrial low-value waste residues was preconized. Hence, carbon dots (C-dots) were synthesized from wastewaters of the cork industry—an abundant and affordable, but environmentally-problematic industrial effluent. The carbon nanomaterials were structurally and morphologically characterised, and their photophysical properties were analysed by an ensemble of spectroscopy techniques. Afterwards, they were successfully applied as highly-sensitive fluorescence probes for the direct detection of haemproteins. Haemoglobin, cytochrome c and myoglobin were selected as specific targets owing to their relevant roles in living organisms, wherein their deficiencies or surpluses are associated with several medical conditions. For all of them, remarkable responses were achieved, allowing their detection at nanomolar levels. Steady-state and time-resolved fluorescence, ground-state UV–Vis absorption and electronic circular dichroism techniques were used to investigate the probable mechanisms behind the fluorescence turn-off of C-dots. Extensive experimental evidence points to a static quenching mechanism. Likewise, resonance energy transfer and collisional quenching have been discarded as excited-state deactivating mechanisms. It was additionally found that an oxidative, photoinduced electron transfer occurs for cytochrome c, the most electron-deficient protein. Besides, C-dots prepared from citric acid/ethylenediamine were comparatively assayed for protein detection and the differences between the two types of nanomaterials highlighted.

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Section: Detection Of Hemeproteinssupporting

confidence: 80%

Section: Understanding the Sensitivity Of C-dots Toward Hemeproteinsmentioning

confidence: 57%

Finding Value in Wastewaters from the Cork Industry: Carbon Dots Synthesis and Fluorescence for Hemeprotein Detection

et al. 2020

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“…Omer et al prepared blue emitting N and P doped CQDs with 0.41% of QY, via hydrothermal method (15 h at 180 • C) by using four major components as precursors including trimesic acid, urea, polyethylene diamine branched, and ortho-phosphoric acid [117]. However, Huang et al applied a low-temperature heating (50 min at 80 • C) strategy to make N and P co-doped CQDs with blue emission and enhanced QY of 12.7%, while using sucrose as C source, and 1,2-ethylenediamine and phosphoric acid as N and P dopants [118]. Moreover, these co-doped CQDs have exhibited excellent fluorescent stability over broad range of pH (4-11), ultra-high ion strength (3 M KCl), and constant UV light irradiation (for 3 h).…”

Section: N P Co-doped Cqdsmentioning

confidence: 99%

Recent Advancements in Doped/Co-Doped Carbon Quantum Dots for Multi-Potential Applications

Kandasamy

2019

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Carbon quantum dots (CQDs)/carbon nanodots are a new class of fluorescent carbon nanomaterials having an approximate size in the range of 2–10 nm. The majority of the reported review articles have discussed about the development of the CQDs (via simple and cost-effective synthesis methods) for use in bio-imaging and chemical-/biological-sensing applications. However, there is a severe lack of consolidated studies on the recently developed CQDs (especially doped/co-doped) that are utilized in different areas of application. Hence, in this review, we have extensively discussed about the recent development in doped and co-doped CQDs (using elements/heteroatoms—e.g., boron (B), fluorine (F), nitrogen (N), sulphur (S), and phosphorous (P)), along with their synthesis method, reaction conditions, and/or quantum yield (QY), and their emerging multi-potential applications including electrical/electronics (such as light emitting diode (LED) and solar cells), fluorescent ink for anti-counterfeiting, optical sensors (for detection of metal ions, drugs, and pesticides/fungicides), gene delivery, and temperature probing.

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“…[ 17–19 ] In comparison with heavy‐metal‐based quantum dots and traditional semiconductor quantum dots, CQDs have been emphasized because of simple synthesis, tunable photoluminescence, water solubility, biocompatibility, low toxicity, and the ease of conjugation with biomolecules. [ 20–22 ] Such excellent features combined in single nano‐dots make them particularly attractive for bioimaging. Currently, efforts have been increasingly focused on using renewable natural resources as the raw materials to prepare CQDs, such as coffee, [ 23 ] pomelo peel, [ 24 ] sugarcane waste, [ 25 ] and tobacco.…”

Section: Introductionmentioning

confidence: 99%

Facile synthesis of phosphorus‐nitrogen doped carbon quantum dots from cyanobacteria for bioimaging

Wang

Gao

et al. 2021

Can J Chem Eng

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Carbon quantum dots (CQDs) are becoming powerful fluorophore materials in the field of biosensors and bioimaging thanks to their small size, optical properties, biocompatibility, and low toxicity. Heteroatom doping can endow CQDs with new or improved photoluminescent properties. In this paper, the bio‐waste cyanobacteria, H3PO4 and C2H8N2 were used as precursors for synthesizing P‐doped, N‐doped and P/N co‐doped CQDs via a simple hydrothermal method. Under the irradiation of ultraviolet light (UV), the optimal emission peaks of undoped CQDs and P‐doped CQDs were located in the blue‐light region, while those of N‐doped CQDs and P/N co‐doped CQDs were located in the green‐light region. Compared with the undoped, N‐doped and P‐doped CQDs, the P/N co‐doped CQDs had higher quantum yield (QY) and more favourable photoluminescent (PL) properties. The as‐prepared P/N co‐doped CQDs were highly fluorescent and exhibited excitation wavelength‐dependent fluorescence; its maximum emission wavelength was at 501 nm under the excitation of 420 nm. Furthermore, the percentage of survival cells kept at 90.4% when the P/N co‐doped CQDs concentration was up to 200 μg/mL by MTS assay. Such P/N co‐doped CQDs were then used as an effective fluorescent probe for cell imaging due to their high QY, good water dispersibility, strong fluorescence properties, fine biocompatibility, and low cytotoxicity.

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Low-temperature rapid synthesis of nitrogen and phosphorus dual-doped carbon dots for multicolor cellular imaging and hemoglobin probing in human blood

Cited by 48 publications

References 33 publications

Finding Value in Wastewaters from the Cork Industry: Carbon Dots Synthesis and Fluorescence for Hemeprotein Detection

Finding Value in Wastewaters from the Cork Industry: Carbon Dots Synthesis and Fluorescence for Hemeprotein Detection

Recent Advancements in Doped/Co-Doped Carbon Quantum Dots for Multi-Potential Applications

Facile synthesis of phosphorus‐nitrogen doped carbon quantum dots from cyanobacteria for bioimaging

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