Low temperature plasma induced apoptosis in CNE‐2Z cells through endoplasmic reticulum stress and mitochondrial dysfunction pathways

Wang, Enjun; Gao, Ying; Wu, Qibing; Rao, Shenghong; Wang, Hongzhi; Bao, Lingzhi

doi:10.1002/ppap.201600249

Cited by 9 publications

(5 citation statements)

References 58 publications

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“…In addition, the quantitative detection results of intracellular free radicals were di cult to obtain accurately with the current technology, and the concentration of free radicals could not be quanti ed (Kalyanaraman et al, 2017).Therefore, in this study, a common uorescent probe was used to re ect the changing trend of free radicals, and the relative uorescence intensity of each experimental group was analyzed with a uorescence microplate reader to re ect the changing trend of intracellular ROS and RNS with the processing time. In fact, whether it was intracellular ROS and RNS or extracellular ROS and RNS, its amount was always positively correlated with processing time, which was consistent with the results of most researchers (Song et al, 2018, Xu et al, 2020, Zhang et al, 2020a.…”

Section: Discussionsupporting

confidence: 90%

Low-Dose Cold Atmospheric Plasma Promoting Schwann Cells Proliferation By Activating PI3K/Akt/mTOR Pathway

Ge¹,

Bao²,

Chen³

et al. 2021

Preprint

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Cold atmospheric plasma (CAP) is an emerging technology that has attracted the attention of many researchers in many fields and disciplines. In this study, a dielectric barrier discharge (DBD) plasma device was used to treat Schwann cells (SCs) cultured in vitro, and the effect of CAP on SCs proliferation was evaluated by cell morphology, cell viability, cell cycle and expression of related proteins in SCs. The results showed that the production of intracellular ROS and RNS increased with the increase of CAP treatment time. Compared with the control group, the proliferation of SCs treated with CAP for less than 14 s significantly increased, and and then gradually decreased. Besides, the cell cycle results also showed that more cells were in the S+G2/M phase at this time.The PI3K/Akt/mTOR pathway was activated by low-dose CAP, and the expression of cyclinD1 was consistent with the trend of cell proliferation. In addition, n-acetyl-L-cysteine (NAC) preconditioning significantly prevented CAP-induced cellular changes. In conclusion, low-dose CAP-induced of SCs proliferation was closely related to the PI3K/Akt/mTOR signaling pathway. This study provides a new idea for the treatment of peripheral nerve injury.

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Section: Discussionsupporting

confidence: 90%

Low-Dose Cold Atmospheric Plasma Promoting Schwann Cells Proliferation By Activating PI3K/Akt/mTOR Pathway

Ge¹,

Bao²,

Chen³

et al. 2021

Preprint

Self Cite

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“…Various forms of plasma sources have been applied to the studies in this field [25,159,160]. Subsequently, more and more studies have found that plasma can effectively kill a variety of cancer cells, such as melanoma, lung cancer, breast cancer, cervical cancer, liver cancer, colon cancer, nasopharyngeal cancer cells, etc, and the killing efficiencies are proven to be dose dependent (shown in table 4) [23,[161][162][163][164][165][166][167][168][169][170][171][172][173][174][175][176][177][178][179][180]. In addition, plasma could also inhibit the growing speed of the tumor bodies (malignant glioma, melanoma, breast cancer, colorectal cancer, etc) in tumorbearing mice (in vivo), thus prolonging the life-span of the mice [23,177,180,181].…”

Section: Plasma Therapy For Cancer Cellsmentioning

confidence: 99%

Applications of atmospheric pressure plasma in microbial inactivation and cancer therapy: a brief review

Liu

et al. 2020

Plasma Sci. Technol.

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Atmospheric pressure cold plasma, with advantages such as high particle activity, no thermal damage, high efficiency and direct and friendly contact with human tissues, is considered to have great potential in biomedical applications. Therefore, 'plasma medicine' as a new interdiscipline has been developed in the past two decades. This review first briefly describes the development of typical plasma sources suitable for biomedical applications, and those with different discharge forms are simply compared, evaluated and summarized. Subsequently, measurement of the crucial gaseous reactive particles (e.g. OH and O) and their spatio-temporal distributions are introduced. Meanwhile, the generation and variation rules and the related critical macroscopic parameters of the plasma-induced aqueous reactive species are summarized. Finally, related studies in the last ten years on the mechanisms of the plasma-driven microbial inactivation and plasma-induced apoptosis of cancer cells are introduced. Moreover, some scientific problems that need to be urgently solved in the field of plasma medicine are also discussed. This review will provide useful guidance for future related research.

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“…In this argon gas generated-plasma, ionization and chemical processes are directly determined by electron temperatures, and therefore is not as sensitive to thermal processes and the gas ion temperature compared to thermal plasma (14) (Figure 1A and B). It has been reported that CAP induces apoptosis in several cancer cells mediated by immunogenic cell death (15)(16)(17). First, the effects of microwave CAP on the viability of colorectal and thyroid cancer cells, HCT-116 and BCPAP, respectively, were examined.…”

Section: Cold Atmospheric Plasma (Cap) Induces Apoptosismentioning

confidence: 99%

Cold Atmospheric Plasma Induces HMGB1 Expression in Cancer Cells

Yoon

Lee

et al. 2019

Anticancer Res

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Background/Aim: Plasma medicine is a new field that provides great potential for the treatment of human diseases including cancer in addition to sterilizing the surface of skin and facilitating wound healing. Recently, nonthermal atmospheric plasma (or cold atmospheric plasma, CAP) was introduced, not only for denaturing cells and tissues, but also for operating under the threshold of thermal damage and for chemically inducing a specific response or modification. Materials and Methods: Microwave-mediated CAP was used in this study. Results: CAP increased highmobility group box 1 protein (HMGB1) expression, thereby increasing HMGB-1 secretion. In addition, we observed that the calreticulin (CRT) protein was concentrated at the cellular membrane when plasma was treated, representing immunogenic cell death. Conclusion: Overall, plasma treatment induces apoptosis via immunogenic cell death in cancer cells, implying a potential application to human cancer therapy and for the treatment of other human diseases.Cancer is the second leading cause of death in the USA, and conventional therapeutic approaches including chemo/radio therapy could not face the current crisis. New technologies, concepts, or combination therapies are needed to improve the current situation. Plasma is an energy that consists of active

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Low temperature plasma induced apoptosis in CNE‐2Z cells through endoplasmic reticulum stress and mitochondrial dysfunction pathways

Cited by 9 publications

References 58 publications

Low-Dose Cold Atmospheric Plasma Promoting Schwann Cells Proliferation By Activating PI3K/Akt/mTOR Pathway

Low-Dose Cold Atmospheric Plasma Promoting Schwann Cells Proliferation By Activating PI3K/Akt/mTOR Pathway

Applications of atmospheric pressure plasma in microbial inactivation and cancer therapy: a brief review

Cold Atmospheric Plasma Induces HMGB1 Expression in Cancer Cells

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