Low temperature cell pausing: an alternative short-term preservation method for use in cell therapies including stem cell applications

Robinson, Nathalie J.; Picken, Andrew; Coopman, Karen

doi:10.1007/s10529-013-1349-5

Cited by 32 publications

(17 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is a wealth of information and large range of protocols available regarding the preservation of cells, including stem cells (the reader is directed to a number of recent reviews: Coopman, 2011;Golab et al, 2013;Hunt, 2011;Robinson et al, 2014;Thirumala et al, 2013). A recent overview of proposals submitted to the FDA for MSCbased products indicated that >80% of 66 submissions considered used cryopreservation to store and transport their product (Mendicino et al, 2014).…”

Section: Can the Cell Therapy Be Preserved And If So How?mentioning

confidence: 99%

“…• C or ambient temperatures, remains limited (Robinson et al, 2014). In particular, despite the fact that cells for cell therapy will more than likely be stored in suspension, most research is focused on how cells adhered to a surface respond to hypothermic conditions, limiting the relevance of these studies.…”

Section: Can the Cell Therapy Be Preserved And If So How?mentioning

confidence: 99%

See 1 more Smart Citation

From production to patient: challenges and approaches for delivering cell therapies

Coopman

Medcalf

2014

StemBook

Self Cite

View full text Add to dashboard Cite

Section: Can the Cell Therapy Be Preserved And If So How?mentioning

confidence: 99%

Section: Can the Cell Therapy Be Preserved And If So How?mentioning

confidence: 99%

From production to patient: challenges and approaches for delivering cell therapies

Coopman

Medcalf

2014

StemBook

Self Cite

View full text Add to dashboard Cite

“…The adhesion ability was poor and CFU capacity was low (8.67 ± 1.67). High level of late stage apoptosis, poor adhesion ability and low CFU capacity showed that cells could not adequately be stored for 48 h. These results may be a consequence of reduced nutrient supply and increase in waste and lactic acid accumulation during prolonged storage (Robinson, Picken & Coopman, 2014). The late stage apoptosis of cells (29.13 ± 3.22%) stored for 24 h was acceptable, and the CFU number (17.07 ± 4.01) of cells stored for 24 h was relatively high.…”

Section: Discussionmentioning

confidence: 99%

Preservation media, durations and cell concentrations of short-term storage affect key features of human adipose-derived mesenchymal stem cells for therapeutic application

Zhang

Ren

Shao

et al. 2017

PeerJ

View full text Add to dashboard Cite

BackgroundAdipose-derived mesenchymal stem cells (ADSCs) have shown great potential in the treatment of various diseases. However, the optimum short-term storage condition of ADSCs in 2∼8 °C is rarely reported. This study aimed at optimizing a short-term storage condition to ensure the viability and function of ADSCs before transplantation.MethodsPreservation media and durations of storage were evaluated by cell viability, apoptosis, adhesion ability and colony-forming unit (CFU) capacity of ADSCs. The abilities of cell proliferation and differentiation were used to optimize cell concentrations. Optimized preservation condition was evaluated by cell surface markers, cell cycle and immunosuppressive capacity.ResultsA total of 5% human serum albumin in multiple electrolytes (ME + HSA) was the optimized medium with high cell viability, low cluster rate, good adhesion ability and high CFU capacity of ADSCs. Duration of storage should be limited to 24 h to ensure the quality of ADSCs before transplantation. A concentration of 5 × 106 cells/ml was the most suitable cell concentration with low late stage apoptosis, rapid proliferation and good osteogenic and adipogenic differentiation ability. This selected condition did not change surface markers, cell cycle, indoleamine 2, 3-dioxygenase 1 (IDO1) gene expression and kynurenine (Kyn) concentration significantly.DiscussionIn this study, ME + HSA was found to be the best medium, most likely due to the supplement of HSA which could protect cells, the physiological pH (7.4) of ME and sodium gluconate ingredient in ME which could provide energy for cells. Duration should be limited to 24 h because of reduced nutrient supply and increased waste and lactic acid accumulation during prolonged storage. To keep cell proliferation and limit lactic acid accumulation, the proper cell concentration is 5× 106 cells/ml. Surface markers, cell cycle and immunosuppressive capacity did not change significantly after storage using the optimized condition, which confirmed our results that this optimized short-term storage condition of MSCs has a great potential for the application of cell therapy.

show abstract

“…This includes hypothermic preservation (i.e. 'cell pausing') at either ambient [5][6][7] or refrigerated chilled temperatures [8,9]. Ultimately, the decision as to which biopreservation method is most appropriate will need to take into account which method has the least effect on the product's CQAs whilst remaining both commercially, clinically and logistically viable.…”

Section: Types Of Biopreservationmentioning

confidence: 99%

The role of biopreservation in cell and gene therapy bioprocessing

Rafiq¹,

Nienow²,

Hewitt³

et al. 2017

Cell Gene Therapy Insights

Self Cite

View full text Add to dashboard Cite

Low temperature cell pausing: an alternative short-term preservation method for use in cell therapies including stem cell applications

Cited by 32 publications

References 42 publications

From production to patient: challenges and approaches for delivering cell therapies

From production to patient: challenges and approaches for delivering cell therapies

Preservation media, durations and cell concentrations of short-term storage affect key features of human adipose-derived mesenchymal stem cells for therapeutic application

The role of biopreservation in cell and gene therapy bioprocessing

Contact Info

Product

Resources

About