Low-temperature biodegradation of high amounts of phenol by Rhodococcus spp. and basidiomycetous yeasts

Margesin, Rosa; Fonteyne, Pierre-Alain; Redl, Bernhard

doi:10.1016/j.resmic.2004.08.002

Cited by 108 publications

(64 citation statements)

References 31 publications

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“…are not significantly different. protein synthesis, membrane permeability, enzyme production, and extent of cellular stress (40,(50)(51)(52)(53). This hypothesis is further supported by a mathematical growth model published by Corkrey et al (54).…”

Section: Discussionmentioning

confidence: 63%

“…The results obtained in this study add a new fact to the already known effect of increased cell yield at low temperatures, which has been reported several times, e.g., by Herbert and Bell (7), Feller et al (8), Isaksen and Jørgensen (11), and Bakermans and Nealson (10), for a wide range of taxonomic groups, e.g., the genera Arthrobacter, Alteromonas, Bacillus, Moraxella, Pseudomonas, Psychrobacter, and Rhodococcus (8,10,39,40). All of these groups are psychrotrophic or psychrophilic isolates from cold habitats (Polar Sea, deep sea, and cold soils).…”

Section: Discussionmentioning

confidence: 73%

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Increased Biomass Production by Mesophilic Food-Associated Bacteria through Lowering the Growth Temperature from 30°C to 10°C

Seel

Derichs

Lipski

2016

Appl Environ Microbiol

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Five isolates from chilled food and refrigerator inner surfaces and closely related reference strains of the species Escherichia coli, Listeria monocytogenes, Staphylococcus xylosus, Bacillus cereus, Pedobacter nutrimenti, and Pedobacter panaciterrae were tested for the effect of growth temperature (30°C and 10°C) on biomass formation. Growth was monitored via optical density, and biomass formation was measured at the early stationary phase based on the following parameters in complex and defined media: viable cell count, total cell count, cell dry weight, whole-cell protein content, and cell morphology. According to the lack of growth at 1°C, all strains were assigned to the thermal class of mesophiles. Glucose and ammonium consumption related to cell yield were analyzed in defined media. Except for the protein content, temperature had a significant (t test, P < 0.05) effect on all biomass formation parameters for each strain. The results show a significant difference between the isolates and the related reference strains. Isolates achieved an increase in biomass production between 20% and 110% at the 10°C temperature, which is 15 to 25°C lower than their maximum growth rate temperatures. In contrast, reference strains showed a maximum increase of only about 25%, and some reference strains showed no increase or a decrease of approximately 25%. As expected, growth rates for all strains were higher at 30°C than at 10°C, while biomass production for isolates was higher at 10°C than at 30°C. In contrast, the reference strains showed similar growth yields at the two temperatures. This also demonstrates for mesophilic bacterial strains more efficient nutrient assimilation during growth at low temperatures. Until now, this characteristic was attributed only to psychrophilic microorganisms. IMPORTANCEFor several psychrophilic species, increased biomass formation was described at temperatures lower than optimum growth temperatures, which are defined by the highest growth rate. This work shows increased biomass formation at low growth temperatures for mesophilic isolates. A comparison with closely related reference strains from culture collections showed a significantly smaller increase or no increase in biomass formation. This indicates a loss of specific adaptive mechanisms (e.g., cold adaptation) for mesophiles during long-term cultivation. The increased biomass production for mesophiles under low-temperature conditions opens new avenues for a more efficient biotechnological transformation of nutrients to microbial biomass. These findings may also be important for risk assessment of cooled foods since risk potential is often correlated with the cell numbers present in food samples.T emperature has an obvious influence on the physiological functions and, consequently, on the growth and survival of bacteria (1, 2). Accordingly, bacteria have adapted to the specific temperature ranges of various environments, which can be summarized in different temperature classes.Cold-adapted microorganisms are classified as ps...

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 73%

Increased Biomass Production by Mesophilic Food-Associated Bacteria through Lowering the Growth Temperature from 30°C to 10°C

Seel

Derichs

Lipski

2016

Appl Environ Microbiol

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“…The clone library produced from the PCR amplification of the ITS region contained members of the genera Trichosporon, Cryptococcus, Rhodotorula, Nectria, Fusarium, Ascomycete and Acremonium. Of these seven genera, isolated strains of Trichosporon, Cryptococcus, Rhodotorula and Fusarium have been shown to degrade phenol (Neujahr and Varga, 1970;Rubin and Schmidt, 1985;Margesin et al, 2003Margesin et al, , 2005Alexievaa et al, 2004;Atagana, 2004;Santos and Linardi, 2004;Bergauer et al, 2005;Krallish et al, 2006). Our attempt to isolate phenol-degrading fungi revealed only Trichosporon as a culturable, phenol-degrading yeast from our site.…”

Section: Discussionmentioning

confidence: 84%

“…Other organisms represented in this dendrogram included members of the genera Cryptococcus, Rhodotorula, Nectria, Fusarium, Ascomycete and Acremonium. Because several prior studies that have found Trichosporon and other fungi to have phenol-degrading capacities (Neujahr and Varga, 1970;Rubin and Schmidt, 1985;Alexievaa et al, 2004;Margesin et al, 2005), we suspected that some, if not all, of the fungi identified in the 13/13 treatment may be primary degraders.…”

Section: Resultsmentioning

confidence: 98%

“…The universal toxicity and ubiquitous nature of phenol make its biodegradation a topic of great interest (ATSDR, 2006). Although the earliest studies of phenol biodegradation focused on bacteria, there have been a growing number of fungi studied for their ability to degrade phenol, including members of the genera Trichosporon, Cryptococcus, Rhodotorula, Rhodococcus, Aspergillus, Penicillium, Cladosporium, Trichoderma, Phanerochaete, Candida and Fusarium (Harris and Ricketts, 1962;Dagley, 1967;Neujahr and Varga, 1970;Gaal and Neujahr, 1979;Rubin and Schmidt, 1985;Alexievaa et al, 2004;Atagana, 2004;Santos and Linardi, 2004;Bergauer et al, 2005;Margesin et al, 2005;Krallish et al, 2006;Singh, 2006;Stoilova et al, 2006;Jiang et al, 2007). Fungi are often able to thrive under environmentally stressed conditions (low nutrient availability, low moisture, low pH and low temperature) and unlike bacteria, they can extend their biomass through environmental matrices through hyphal growth, making their potential for metabolism of organic pollutants particularly promising (Buchan et al, 2003;Atagana, 2004;Margesin et al, 2005;de Boer et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Stable isotope probing reveals Trichosporon yeast to be active in situ in soil phenol metabolism

DeRito

Madsen

2008

The ISME Journal

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The aim of this study was to extend the results of our previous stable isotope probing (SIP) investigation: we identified a soil fungus involved in phenol biodegradation at an agricultural field site. DNA extracts from our previous study were examined using fungi-specific PCR amplification of the 18S-28S internal transcribed spacer (ITS) region. We prepared an 80-member clone library using PCR-amplified, 13 C-labeled DNA derived from field soil that received 12 daily doses of 13 C-phenol. Restriction-fragment-length-polymorphism screening and DNA sequencing revealed a dominant clone (41% of the clone library), the ITS sequence of which corresponded to that of the fungal genus Trichosporon. We successfully grew and isolated a white, filamentous fungus from site soil samples after plating soil dilutions on mineral salts agar containing 250 p.p.m. phenol. Restreaking on both yeast extract-peptone-galactose and Sabouraud dextrose agar plates led to further purification of the fungus, the morphological characteristics of which matched those of the genus Trichosporon. The ITS sequence of our isolated fungus was identical to that of a clone from our SIP-based library, confirming it to be Trichosporon multisporum. High-performance liquid chromatography and turbidometeric analyses showed that the culture was able to metabolize and grow on 200 p.p.m. phenol in an aqueous mineral salts medium within 24 h at room temperature. Gas chromatographymass spectrometry analysis of 13 CO 2 respiration from laboratory soil incubations demonstrated accelerated phenol mineralization in treatments inoculated with T. multisporum. These findings show that T. multisporum actively degraded phenol in our field-based, soil experiments.

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Degradation of phenol by aerobic granules and isolated yeast Candida tropicalis

Adav

Chen

Lee

et al. 2006

Biotech & Bioengineering

149

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Aerobic granules effectively degrade phenol at high concentrations. This work cultivated aerobic granules that can degrade phenol at a constant rate of 49 mg-phenol/g x VSS/h up to 1,000 mg/L of phenol. Fluorescent staining and confocal laser scanning microscopy (CLSM) tests demonstrated that an active biomass was accumulated at the granule outer layer. A strain with maximum ability to degrade phenol and a high tolerance to phenol toxicity isolated from the granules was identified as Candida tropicalis via 18S rRNA sequencing. This strain degrades phenol at a maximum rate of 390 mg-phenol/g x VSS/h at pH 6 and 30 degrees C, whereas inhibitory effects existed at concentrations >1,000 mg/L. The Haldane kinetic model elucidates the growth and phenol biodegradation kinetics of the C. tropicalis. The fluorescence in situ hybridization (FISH) and CLSM test suggested that the Candida strain was primarily distributed throughout the surface layer of granule; hence, achieving a near constant reaction rate over a wide range of phenol concentration. The mass transfer barrier provided by granule matrix did not determine the reaction rates for the present phenol-degrading granule.

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Low-temperature biodegradation of high amounts of phenol by Rhodococcus spp. and basidiomycetous yeasts

Cited by 108 publications

References 31 publications

Increased Biomass Production by Mesophilic Food-Associated Bacteria through Lowering the Growth Temperature from 30°C to 10°C

Increased Biomass Production by Mesophilic Food-Associated Bacteria through Lowering the Growth Temperature from 30°C to 10°C

Stable isotope probing reveals Trichosporon yeast to be active in situ in soil phenol metabolism

Degradation of phenol by aerobic granules and isolated yeast Candida tropicalis

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