Low-Temperature Adapted Nitrifying Microbial Communities of Finnish Wastewater Treatment Systems

The membrane-aerated biofilm reactor (MABR) is a novel method for the biological treatment of wastewaters and has been successfully applied for nitrification. To improve the design and adaptation of MABR processes for colder climates and varying temperatures, the temperature dependence of a counter-diffusional biofilm’s nitrification performance was investigated. A lab-scale MABR system with silicone hollow fibre membranes was operated at various temperatures between 8 and 30°C, and batch tests were performed to determine the ammonia oxidation kinetics. Biofilm samples were taken at 8 and 24°C and analysed with 16S rRNA sequencing to monitor changes in the microbial community composition, and a mathematical model was used to study the temperature dependence of mass transfer. A high nitrification rate (3.08 g N m–2 d–1) was achieved at 8°C, and temperature dependence was found to be low (θ = 1.024–1.026) compared to suspended growth processes. Changes in the community composition were moderate, Nitrospira defluvii remaining the most dominant species. Mass transfer limitations were shown to be largely responsible for the observed trends, consistent with other biofilm processes. The results show that the MABR is a promising technology for low temperature nitrification, and appropriate management of the mass transfer resistance can optimise the process for both low and high temperature operation.

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“…and Nitrospira sp. being the most abundant nitrifying bacteria in activated sludge ( Kruglova et al, 2020 ) in Nordic regions.…”

Section: Discussionmentioning

confidence: 99%

Temperature dependence of nitrification in a membrane-aerated biofilm reactor

et al. 2023

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“…Burkholderiales, Clostridiales, and Gaiellales are key orders in the deep bedrock biosphere, ,, and Neisseriales might originate from exogenous microbial sources and is thought to be associated with groundwater . In addition, some increased orders might be closely related to nitrogen metabolism, including Blastocatellales, Rhodocyclales, Nitrosomonadales, Deinococcales, and Micrococcales, which may be due to the increased soluble nitrogen in soil extractions. In parallel, exogenous soil_2 can also potentially affect the decomposition and methane production processes of coal seams.…”

Section: Discussionmentioning

confidence: 99%

Subsurface Microbial Invasion Affects the Microbial Community of Coal Seams

Liu

Yuan

et al. 2021

Energy Fuels

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Coal seam microorganisms have attracted much attention for their special functions that are closely related to secondary biogenic gas formation, but the influence of subsurface microbial invasion on coal microorganisms remains poorly understood. In this study, subsurface microorganisms from soils at different depths were used to infect coal samples, and the abundance and composition of the microbial community were analyzed by quantitative polymerase chain reaction (PCR) and high-throughput sequencing. The results showed that methane production from the coal samples treated with soil extractions did not increase significantly nor did the abundance and transcriptional activity of archaeal 16S rRNA and mcrA genes. In contrast, two soil extractions significantly improved the abundance and transcriptional activity of the coal bacteria. There was no significant difference in the effects of different soil extractions on bacterial abundance and transcriptional activity, but differences in the effects on α- and β-bacterial diversity between the coal samples treated by different soil extractions were found. Soil extraction from the shallow layer soil far away from the coal mine increased the bacterial α-diversity of the coal samples and changed the bacterial community composition. In addition, this soil extraction increased various bacterial groups in the coal sample and changed the predictive metagenome functional contents of the coal samples from the bacterial sequence data. In summary, this study provides basic microbial information for subsurface microbial invasion of coal seams and helps increase understanding of the source of microorganisms in in situ coal seams and the changes in the microbial community during subsurface microbial migration.

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“…Nitrotoga turned out to belong to the core community and outcompeted Nitrospira in some of the plants (Lücker et al 2015 ; Chen et al 2020 ; Saunders et al 2016 ). Its relative 16S rRNA gene sequence abundance can reach 0.5–2% or even 4% (Johnston et al 2019 ; Numberger et al 2019 ; Saunders et al 2016 ; Kruglova et al 2020 ). In reactor systems for the treatment of ammonia-contaminated airstreams operated at 10 °C, Cand.…”

Section: Competition Of Cand Nitrotoga With Nitrospira and Nitrobactermentioning

confidence: 99%

“…The highest cell numbers of Cand. Nitrotoga were observed in late winter or in spring (Keene et al 2017 ; Miłobędzka and Muszyński 2017 ; Numberger et al 2019 ; Kruglova et al 2020 ; Kim et al 2021 ) when the temperature is still too low for optimal growth of other NOB. A temperature-dependent shift in the NOB community was confirmed in a bioreactor experiment with inorganic mine waters (Table S1 ; Karkman et al 2011 ).…”

Section: Competition Of Cand Nitrotoga With Nitrospira and Nitrobactermentioning

confidence: 99%

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Relevance of Candidatus Nitrotoga for nitrite oxidation in technical nitrogen removal systems

Spieck

Wegen

Keuter

2021

Appl Microbiol Biotechnol

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Many biotechnological applications deal with nitrification, one of the main steps of the global nitrogen cycle. The biological oxidation of ammonia to nitrite and further to nitrate is critical to avoid environmental damage and its functioning has to be retained even under adverse conditions. Bacteria performing the second reaction, oxidation of nitrite to nitrate, are fastidious microorganisms that are highly sensitive against disturbances. One important finding with relevance for nitrogen removal systems was the discovery of the mainly cold-adapted Cand. Nitrotoga, whose activity seems to be essential for the recovery of nitrite oxidation in wastewater treatment plants at low temperatures, e.g., during cold seasons. Several new strains of this genus have been recently described and ecophysiologically characterized including genome analyses. With increasing diversity, also mesophilic Cand. Nitrotoga representatives have been detected in activated sludge. This review summarizes the natural distribution and driving forces defining niche separation in artificial nitrification systems. Further critical aspects for the competition with Nitrospira and Nitrobacter are discussed. Knowledge about the physiological capacities and limits of Cand. Nitrotoga can help to define physico-chemical parameters for example in reactor systems that need to be run at low temperatures. Key points • Characterization of the psychrotolerant nitrite oxidizer Cand. Nitrotoga • Comparison of the physiological features of Cand. Nitrotoga with those of other NOB • Identification of beneficial environmental/operational parameters for proliferation

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Low-Temperature Adapted Nitrifying Microbial Communities of Finnish Wastewater Treatment Systems

Cited by 14 publications

References 24 publications

Temperature dependence of nitrification in a membrane-aerated biofilm reactor

Temperature dependence of nitrification in a membrane-aerated biofilm reactor

Subsurface Microbial Invasion Affects the Microbial Community of Coal Seams

Relevance of Candidatus Nitrotoga for nitrite oxidation in technical nitrogen removal systems

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