Low sodium isocyanurate concentrations as a substitute to medium autoclaving in plant tissue culture

Urtiga, Caio da Costa; Silva‐Cardoso, Inaê Mariê de Araújo; Figueiredo, S. A.

doi:10.1007/s11240-019-01681-9

Cited by 7 publications

(4 citation statements)

References 11 publications

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“…This fact is further evidence of reduced or no phytotoxic effect. In any case, the contamination rate has never been over 5% (da Costa Urtiga et al, 2019).…”

Section: Potential Advantages Of Nadcc In Plant Tissue Culturementioning

confidence: 91%

“…The overall idea is to bypass autoclaving, which is a time-consuming procedure apart from the cost of electricity, infrastructure availability and human expertise. Therefore, it is logical to use a chemical that has signi icantly less or negligible environmental toxicity to prevent contaminating microorganisms in tissue culture media (da Costa Urtiga et al, 2019). The use of such an agent will also reduce the cost of plant tissue culture enterprise.…”

Section: Potential Advantages Of Nadcc In Plant Tissue Culturementioning

confidence: 99%

“…The use of such an agent will also reduce the cost of plant tissue culture enterprise. NaDCC is having all the ideal properties, including stability, low toxicity and ease of handling (da Costa Urtiga et al, 2019). The potential application of chlorine disinfectants in plant tissue culture sterilisation was irst experimented by Yanagawa et al (2007) for micropropagation of ornaments such as Cymbidium plantlets and Phalaenopsis plantlets through direct regeneration.…”

Section: Potential Advantages Of Nadcc In Plant Tissue Culturementioning

confidence: 99%

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Sodium Dichloroisocyanurate: An eco-friendly chemical alternative for media autoclaving and explant sterilisation in plant tissue culture

Shetty¹,

Narasimhan²

2021

ijrps

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Autoclaving nutrient media is still considered as the optimum mode of sterilisation in plant cell and tissue culture. During the process steam under high pressure is maintained at 120 degrees Celsius, 15 psi for 15-20 minutes in a chamber, optimised to kill all possible microbial life forms. But the disadvantages related to the process of autoclaving are plentiful. They are, decrease in the media pH, salt precipitation, agar depolymerisation, carbohydrate hydrolysis, volatile obliteration and necessity of the infrastructure investment. Requirements of additional resources (time, human resources, electrical energy) have forced the lookout for a more viable alternative, that is, chemical sterilisation. The use of Sodium dichloroisocyanurate (NaDCC) is a useful alternative for media and explant sterilisation. NaDCC is stable, water-soluble, non-toxic and easy to use at room temperature, does not have any environmental hazards and is not phytotoxic. The use of NaDCC as a disinfectant has been documented well concerning water sterilisation, surface sterilisation and also as a broad spectrum disinfecting agent. Disinfecting property of NaDCC is due to the hydrolytic release of chlorine, and this can be utilised for sterilisation of media and explants in plant tissue culture. NaDCC is a useful alternative for autoclaving at a concentration range of 0.05 to 1.0 g/l. However, only a few reports are available for its use as a sterilising agent for media and explants for in vitro cultures of plants. This paper discusses and reviews the possibility of establishing NaDCC as an active agent for explant sterilisation and as a viable alternative to medium sterilisation through autoclaving.

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“…This fact is further evidence of reduced or no phytotoxic effect. In any case, the contamination rate has never been over 5% (da Costa Urtiga et al, 2019).…”

Section: Potential Advantages Of Nadcc In Plant Tissue Culturementioning

confidence: 91%

Section: Potential Advantages Of Nadcc In Plant Tissue Culturementioning

confidence: 99%

Section: Potential Advantages Of Nadcc In Plant Tissue Culturementioning

confidence: 99%

See 1 more Smart Citation

Sodium Dichloroisocyanurate: An eco-friendly chemical alternative for media autoclaving and explant sterilisation in plant tissue culture

Shetty¹,

Narasimhan²

2021

ijrps

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show abstract

“…The use of autoclaving for the sterilization of culture media, glassware, and utensils used in micropropagation is another factor that helps increase costs, due to electric energy consumption and equipment maTntenance expenses, along with the fact that it requires more work hours (Pais et al, 2016;da Costa Urtiga, 2019: Lu et al, 2021.…”

Section: Introductionmentioning

confidence: 99%

Xanthan gum and sodium hypochlorite in vitro rooting of Gerbera hybrida cv. Essandre

Ribeiro

Teixeira

et al. 2022

RSD

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The aim of this study was to check the use of the following gelling agents: the xanthan gum “Adicel®” and the stabilizer “Super Liga Neutra®” to replace agar in the in vitro rooting phase of Gerbera hybrida cv. Essandre. Additionally, the possibility of using chemical sterilization of both culture media and glassware with sodium hypochlorite to replace autoclaving was analyzed. The gelling agents, xanthan gum “Adicel®” and the stabilizer “Super Liga Neutra®” were tested at the following concentrations (g L-1): 7; 9; 11; 13; 15; 17; 19; and 21. No concentrations of Super Liga Neutra® provided effective solidification. Concentrations of 17 and 21 g of Adicel® provided a good gelling of the culture medium, which was compared to the medium containing agar (control) with two types of sterilization: autoclaving (for 20 and 40 minutes) and chemical sterilization. Autoclaving for 20 minutes did not provide effective elimination of contamination in the culture medium containing xanthan gum; this only occurred when autoclaving time increased to 40 minutes. Plant development in culture media containing 17 and 21 g of xanthan gum, either sterilized by autoclaving for 40 minutes or at a concentration of 17 g xanthan gum using sodium hypochlorite, was statistically the same as the control that contained agar. However, plant development at a concentration of 21g of xanthan gum in a sterilized medium using sodium hypochlorite was lower than that observed in media containing agar.

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Comparison of Performance of Cold Atmospheric Plasma and Biocides on the Inactivation of Alternaria sp.

Alves

Lima

Vitoriano

et al. 2022

IEEE Trans. Plasma Sci.

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Low sodium isocyanurate concentrations as a substitute to medium autoclaving in plant tissue culture

Cited by 7 publications

References 11 publications

Sodium Dichloroisocyanurate: An eco-friendly chemical alternative for media autoclaving and explant sterilisation in plant tissue culture

Sodium Dichloroisocyanurate: An eco-friendly chemical alternative for media autoclaving and explant sterilisation in plant tissue culture

Xanthan gum and sodium hypochlorite in vitro rooting of Gerbera hybrida cv. Essandre

Comparison of Performance of Cold Atmospheric Plasma and Biocides on the Inactivation of Alternaria sp.

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