Low Serum Paraoxonase-1 Lactonase and Arylesterase Activities in Obese Children and Adolescents

Abstract

Serum paraoxonase-1 (PON1) binds mainly to high density lipoproteins (HDLs)

Vitamin K Dependent Proteins in Kidney Disease

“…Thus, the enhanced expression of MGP by exposure to reactive oxygen species leads to atherogenic stimuli. Several studies were conducted in adults and children [123] to find a better marker for oxidative stress and it would be an interesting direction for future studies to assess the relationship between MGP and oxidative stress in patients with kidney disease.…”

Biomarkers in Obesity

“…The Oxidative Stress status in obesity is another demonstrated condition which influences the impact of obesity on the cardiovascular system and insulin resistance (7,13). To date, no validated biomarkers to measure this status are used in clinical practice, although clinical research is developed in this field (7,14).…”

Malondialdehyde levels can be measured in serum and saliva by using a fast HPLC method with visible detection / Determinarea printr-o metodă HPLC-VIS rapidă a concentraţiilor serice şi salivare ale malondialdehidei

“…One of the most studied biomarkers of the lipid peroxidation process is malondialdehyde (MDA). It can be considered not only a biomarker but even an "ultimate toxicant" of the hydroxyl radical [3,[5][6][7][8].…”

“…The most commonly and widely available method is based on the reaction of malondialdehyde with thiobarbituric acid when a red condensation product with a maximum of absorption at 532 nm is formed [9][10][11][12][13]. It can be detected spectrophotometrically after extraction with organic solvents (n-butanol, isobutyl alcohol) [14], it can be analyzed by commercial kits [8,[15][16][17]. The major problems with this method are the lack of sensitivity and the occurrence of byproducts because the byproducts absorb light near 532 nm (maximum of absorption of MDA-(TBA) 2 ) [18,19].…”